Ikhithi yokuxilonga yeMyoglobin (i-fluorescence immunochromatographic assay)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-myoglobin (i-fluorescence immunochromatographic assay) luvavanyo lwe-fluorescence immunochromatographic yokufumanisa ubuninzi be-concentration ye-myoglobin (MYO) kwi-serum yabantu okanye i-plasma, esetyenziswa kakhulu njengoncedo ekuxilongweni kwe-acute myocardial infarction. Olu vavanyo lwenzelwe ukusetyenziswa kwezempilo kunye nokusetyenziswa kobuchwephesha basekhaya kuphela.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-MYO ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti MYO antibody kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampulu, i-antigen ye-MYO kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-MYO antibody, kwaye yenze umxube wokugonyeka. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa intsonkothile iphumelele ummandla wovavanyo, idityaniswe ne-anti-MYO yokugquma i-antibody, yenza into entsha. Inqanaba le-MYO linxibelelene ngokufanelekileyo kunye nomqondiso we-fluorescence, kwaye ukuxinwa kwe-MYO kwisampulu kunokubonwa ngovavanyo lwe-fluorescence immunoassay.