Ikhithi yokuxilonga yeMyoglobin (i-fluorescence immunochromatographic assay)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo kolu fakelo lwephakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-myoglobin (i-fluorescence immunochromatographic assay) luvavanyo lwe-fluorescence immunochromatographic yokufumanisa ubuninzi be-concentration ye-myoglobin (MYO) kwi-serum yabantu okanye i-plasma, esetyenziswa kakhulu njengoncedo ekuxilongweni kwe-acute myocardial infarction. Olu vavanyo lwenzelwe ukusetyenziswa kwezempilo kunye nokusetyenziswa kobuchwephesha basekhaya kuphela.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-MYO ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti MYO antibody kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampulu, i-antigen ye-MYO kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-MYO antibody, kwaye yenze umxube wokugonyeka. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa intsonkothile iphumelele ummandla wovavanyo, idityaniswe ne-anti-MYO yokugquma i-antibody, yenza into entsha. Inqanaba le-MYO linxibelelene ngokufanelekileyo kunye nomqondiso we-fluorescence, kwaye ukuxinwa kwe-MYO kwisampulu kunokubonwa ngovavanyo lwe-fluorescence immunoassay.