Umgaqo

I-membrane yesixhobo sovavanyo idityaniswe ne-Anti Ev71 kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-anti I-pad ibuthathaka idityaniswe yi-fluorescence elenzelwe i-antiv71 i-antidy kunye ne-rabit kunye ne-Igg ye-Igg kwangaphambili. Xa uvavanya isampulu, i-antign ye-antign kwisampulu idibanise i-fluorescence elebheli ye-Anti Ev71, kwaye ibunjwe ngumxube. Phantsi kwesenzo se-chromatography, ukuhamba okuntsonkothileyo kwicala le-antictant, xa intsomi egqithayo ingingqi yovavanyo, idityaniswa ne-Antiv71 i-antidy av.

Ukuba ayinanto, isampulu ayiqokeleli i-Enterovirus 71 I-Igm antidy, ukuze kwenzeke ukuba amajokweni omzimba akanakwenziwa. Akuyi kubakho mgca obomvu kwindawo yokuchonga (t). Nokuba i-enterovirus i-Igm ikhula kwi-Supedn okanye hayi, i-Count-Gold-Gold God-Gold Anti-God GEM-MOOCOMNAL Anti kunye ne-GoA-Anti-Mouse Anti. Emva koko i-ageglutinates iphuhlisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kuvela (c). Umgca obomvu ngumgangatho omiselweyo ubonakala kwindawo yoLawulo loMgangatho (c) wokugweba ukuba kukho iisampulu ezaneleyo nokuba i-chromatography yenkqubo iqhelekile. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi wee-regentnts.

Inkqubo yovavanyo:

1.Iza neesampuli ezivavanyiweyo zinokuba yigazi liphela, kubandakanya negazi elinomsindo okanye igazi eliphakamileyo. Igazi liphela alinakugcinwa emva kokuqokelela. Kuya kufuneka ndisetyenziswe kwakamsinya emva kokuqokelela.

Iisampulu ze-2.Fusim ziqokelelwa ngokwengqondo ngokweendlela ezisemgangathweni. I-serum engafakwanga i-SUMS ayinakho ukusebenzisa. Akucetyiswa ukuba usebenzise i-lpeipec, i-turbid okanye i-serum engcolisekileyo. Thabatha umcimbi kwiSluum. Kwaye ukuchama kuya kuchaphazela iziphumo zovavanyo, iisampulu ezinjalo kufuneka zibe ze-secedwagigid okanye ezihlanjululwe ngaphambi kokusetyenziswa.

3.Izimpula ezivavanyiweyo zinokuba yi-heparin, iSodium Metic okanye i-EDTA Anticoiclant iplasma.

4.Ukuba iindlela ezisemgangathweni ziqokelela isampulu. I-serum okanye isampulu ye-plasma inokugcinwa kwi-2-8 ℃ kwiintsuku ezi-3 kunye ne-crtooprest engezantsi -10 ° C kwiinyanga ezintathu.

5.Umzekelo waphendukisa ukukhululeka-hlokhole.