UMGAQO

Inwebu yesixhobo sovavanyo igqunywe nge-anti EV71 ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti EV71 antibody kunye ne-IgG yomvundla kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-EV71 kwisampulu idibanisa ne-fluorescence ebhalwe i-anti EV71 antibody, kwaye yenza umxube wokuzivikela komzimba. Ngaphantsi kwesenzo sechromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti EV71 yokugquma i-antibody, yenza into entsha.

Ukuba i-negative, isampuli ayinayo i-enterovirus 71 IgM antibody, ukwenzela ukuba i-immune complex ingenziwa. Akuyi kubakho umgca obomvu kwindawo yokufumanisa (T). Nokuba ikhona i-antibody ye-Enterovirus 71 IgM kumzekelo okanye ayikho, i-antibody eseleyo ye-colloidal yegolide ene-anti-human IgM monoclonal kunye ne-anti-mouse ye-IgG yebhokhwe egqunywe kwindawo yolawulo lomgangatho (C) iyabophelela. Emva koko i-agglutinates iphuhlisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kubonakala kwi (C). Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo:

1.Iisampulu ezivavanyiweyo zingaba ligazi elipheleleyo, kubandakanywa igazi le-venous okanye igazi le-Peripheral. Igazi elipheleleyo alinakugcinwa emva kokuqokelela. Kufuneka ndisetyenziswe ngokukhawuleza emva kokuqokelela.

I-2.Iisampulu zeSerum ziqokelelwa ngokwe-aseptically ngokweendlela eziqhelekileyo. Iserum engasebenziyo ubushushu ayinakusetyenziswa. Akukhuthazwa ukusebenzisa i-lipemic, i-turbid okanye i-serum engcolileyo. Yinto encinci kwiserum. Kwaye imvula iya kuchaphazela iziphumo zovavanyo, iisampulu ezinjalo kufuneka zifakwe kwi-centrifuged okanye zihluzwe ngaphambi kokusetyenziswa.

I-3.Iisampulu ezivavanyiweyo zingaba yi-heparin, i-Sodium citrate okanye i-EDTA i-anticoagulant plasma.

4.Ngokungqinelana neendlela eziqhelekileyo zokuqokelela isampuli. I-Serum okanye isampuli ye-plasma inokugcinwa efrijini kwi-2-8 ℃ iintsuku ezi-3 kunye ne-cryopreservation engaphantsi kwe-15 ° C kwiinyanga ezi-3.

5.Zonke iisampulu ziphephe imijikelo yokunyibilika komkhenkce.