Umgaqo

I-membrane yesixhobo sovavanyo sidityaniswe neQela le-rotavirus i-Antign kummandla wovavanyo kunye nebhokhwe i-goat antibibit igwebu I-padble ibuthathaka idityaniswe yi-fluorescence elenzelwe i-Anti Rotavirus A kunye ne-Rabbit Igg kwangaphambili. Xa uvavanya isampulu, i-RV kwisampulu idibanisa i-fluorescence lalebheli i-Anti Rotavirus A, kunye nomxube oqhelekileyo. Phantsi kwesenzo se-igonockographromatographrography, ukuhamba okuntsonkothileyo kwicala lephepha lokufumana. Xa intsonkoso edlula ingingqi yovavanyo, idityaniswa neqela elichasene ne-anti-rotavirus i-anti yonk 'i-anting, intshabalalo entsha. Ukuba ayinanto, akukho qela leRotavirus i-antigen kwisampulu, ukuze kungasekwa amajoni, kungabikho mgca ubomvu kwindawo yokuchonga (t). Nokuba ingaba i-rotavirus ifumaneka kwi-Speciamen, i-Latex-Ilebhu ye-chromatoyitha yindawo yokulawula umgangatho (c) kwaye ifakwe yi-Goat Anti-Igg Anti. Umgca obomvu uya kuvela kwindawo yokulawula umgangatho (c). Umgca obomvu ngumgangatho omiselweyo ubonakala kwindawo yoLawulo loMgangatho (c) wokugweba ukuba kukho iisampulu ezaneleyo nokuba i-chromatography yenkqubo iqhelekile. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi wee-regentnts.

Inkqubo yovavanyo:

Izigulana ze-1.SMMMMMMMMMOTIC kufuneka ziqokelelwe. Ngokweengxelo, eyona nto iphambili kwi-rotavirus ephezulu yezigulana ezine-geesroenteritis yenzeka kwiintsuku ezingama-3-5 emva kokuqala kwesi sifo kunye neentsuku ezi-3 ukuya kwezi-12 emva kokuqala kweempawu. Ukuba isampulu iqokelelwe ixesha elide emva kokuba iswekile, inani lee-adoulns alinakwanela ukuba lenzeke ngendlela efanelekileyo.

2. Iisampulu kufuneka ziqokelelwe kwisikhongozeli esicocekileyo, esomileyo, esinqabileyo, esingayiqhiphuliyo isicoci kunye nokugcinwa kwezinto ezigciniweyo.

3.fonte izigulana ezingesosifo seengcongconi, iisampulu ze-faeuds akufuneki zibe ngaphantsi kwe-1-2 yeegram. Kwizigulana ezinesifo sohudo, ukuba i-faeps ilulwelo, nceda uqokelele ubuncinci i-1-2 ml ye-faeces. Ukuba amagumbi aqulathe igazi elininzi kunye ne-mucus, nceda uqokelele isampulu kwakhona.

4. Kucetyiswa ukuvavanya iisampulu kwangoko emva kokuqokelelwa, kungenjalo kuya kuthunyelwa elebhu ngaphakathi kweeyure ezi-6 kwaye igcinwe nge-2-5 ° C. Ukuba iisampuli azikhange zivavanywe kwiiyure ezingama-72, kufuneka zigcinwe kwiqondo lobushushu elingezantsi -15 ° C.

Ukugcina ii-feeds ezitsha zokuvavanywa, kunye neesampulu ze-faeces ezixutywe ne-dilungunt okanye amanzi