Umgaqo

I-membrane yesixhobo sovavanyo sidityaniswe ne-microalbumin Antigen kummandla wovavanyo kunye nebhokhwe i-anti cabbit igubit antin antid anti yolawulo. I-padble ibuthathaka idityaniswe yi-fluorescence elila i-microalbumin kunye ne-rabibit igg kwangaphambili. Ukuba akukho albumin kumchamo, i-colloidal yegolide-i-anti-alb-ilebhile enee-anticlon kwiPhepha leGolide leGolide liza kuqhuba kwi-membrane ngomsitho odibeneyo umgca. Kwaye umbala womgca umnyama ngaphezu kombala womgca kwindawo yolawulo (c), esi sisiphumo esibi. Ukuba umchamo uqukethe i-albhamun, baya kukhuphisana ne-Antign enama-Antigen kwi-membrane ukuba babophelele kwiisayithi ezinomda kwi-colloidal yegolide eneelebheli. Njengesixa se-albhamun kumchamo onyukayo, kuvavanyo

Umbala womgca uya kuba lula kwaye ulula. Umxholo we-albhamun emchani unokufunyanwa nge-semi-ngobuninzi ngokuthelekisa inqaku (t) lendawo yolawulo (c). Indawo yoLawulo loMgangatho (c) kunye nendawo ekubhekiswa kuyo (R) kwikhithi iya kuhlala ivela ngexesha lovavanyo, kwaye ayinanto yakwenza nobukho beAlbumin. Indawo yolawulo (c) kunye nendawo ekubhekiswa kuyo (R) isetyenziswe njengesalathiso somgangatho wolawulo lwangaphakathi lwekhithi yeKit.

Inkqubo yovavanyo:

Nceda ufunde isixhobo sokusebenza kwesixhobo kunye nokufakwa kwephakheji ngaphambi kovavanyo. Iisampulu zegumbi kubushushu begumbi ngaphambi kokusetyenziswa.

1.Teke ikhadi lovavanyo kwingxowa ye-foil. Yibeke iflethi kumphezulu othe tyaba kunye nophawu.

2.Funda isampulu yomchamo kunye nombhobho wokulahla, ulahle amaconsi ezimbini okuqala kwesampulu yourune. Yongeza amaconsi ama-3 (malunga ne-til) yomchamo we-bubble-free ukuya embindini womngxunya wekhadi lovavanyo ngokuthe nkqo kwaye qala ixesha.

3.Cumisa umphumo kwimizuzu eli-10 ukuya kweli-15. Ayisebenzi ukuba ngaphezulu kwemizuzu eli-15.