"Ngokusekwe kwimarike yasekhaya kunye nokwandisa ishishini laphesheya" sisicwangciso sethu sokuphucula uMgangatho oPhezulu we-China Bovine Viral Diarrhea Virus Virus Antibody Bvdv Ab Rapid Diagnostic Test, Siza kuxhobisa abantu ngokunxibelelana kunye nokuphulaphula, Ukumisela umzekelo kwabanye nokufunda kumava. .
“Ngokusekwe kwimarike yasekhaya kunye nokwandisa ishishini laphesheya” sisicwangciso sethu sokuphuculaUVavanyo lweNtsholongwane yeSirhudo yaseTshayina, Uvavanyo lwesifo sohudo lwentsholongwane, Umsebenzi wethu wenkampani kukubonelela ngekhwalithi ephezulu kunye neemveliso ezintle ngexabiso elifanelekileyo kwaye sizame ukufumana i-100% yodumo oluhle kubathengi bethu. Sikholelwa ukuba iProfessional ifezekisa ukugqwesa! Siyakwamkela ukuba usebenzisane nathi kwaye nikhule kunye.
Ikhithi yokuxilonga ye-Estradiol(uvavanyo lwe-fluorescence immunochromatographic)
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo kolu fakelo lwephakheji.

UKUSETYENZISWA OKUHLOSIWEYO
I-Diagnostic Kit ye-Estradiol (i-fluorescence immunochromatographic assay) yi-fluorescence immunochromatographic assay yokufumanisa ubungakanani be-Estradiol (E2) kwi-serum yabantu okanye i-plasma, esetyenziselwa ukuvavanya amanqanaba e-Estradiol. kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO
I-Estradiol (E2) yeyona hormone ibaluleke kakhulu kunye neyona nto isebenzayo kwi-estrogen.Isisindo sayo se-molecular yi-272.3 D. Ngokubanzi, kubasetyhini abangakhulelwanga, i-E2 ikhutshwe ngokukodwa ngamaseli e-sheath kunye ne-granular kunye neeseli ze-luteal ngexesha lophuhliso lwe-follicular.Ngexesha lokukhulelwa, I-E2 ikhutshwa ikakhulu yi-placenta, ngeli lixa amadoda eveliswa ikakhulu ngamasende.Emva kokuba i-E2 ingene egazini, i-1% ukuya kwi-3% ayibopheleli kwiiproteni, i-40% ibophelela kwihomoni yesini ebopha iglobulin(SHBG),kwaye abanye bayabophelela I-albumin, i-metabolize esuka esibindini ibe yi-sulfates enyibilikayo emanzini okanye i-gluconaldehyde esters, kwaye ikhutshwe kumchamo.

UMGAQO WENKQUBO
I-membrane yesixhobo sokuvavanya ifakwe kwi-conjugate ye-BSA kunye ne-Estradiol kummandla wokuvavanya kunye nebhokhwe yokulwa nomvundla we-IgG kwingingqi yokulawula. Iphedi yokumakisha iqatywe nge-fluorescence uphawu lwe-anti E2 kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu, i-E2 kwisampulu idibanisa kunye ne-fluorescence ephawulwe nge-anti-E2 antibody, kwaye yenze umxube wokugonyeka. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elifunxayo, xa i-complex iphumelele ummandla wokuvavanya, I-marker ye-fluorescent yamahhala iya kudibaniswa ne-Estradiol kwi-membrane. Uxinzelelo lwe-Estradiol kwisampulu lunokubonwa nge-fluorescence immunoassay assay.

IIREGENTS KUNYE NEZINTO EZIBONISWAYO

Amacandelo ephakheji ye-25T:
.Ikhadi lovavanyo lwefoyile nganye efakwe kwi-desiccant 25T
.Isisombululo 25T
.B isisombululo 1
.Fakela ipakethe 1

IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Isampulu yokuqokelela isitya,ixesha

UKUQOKELELWA KWEsampuli NOKUGCINWA
1.Iisampulu ezivavanyiweyo zingaba serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2.Ngokungqinelana neendlela eziqhelekileyo zokuqokelela isampuli. I-Serum okanye isampuli ye-plasma inokugcinwa efrijini kwi-2-8 ℃ ye-7days kunye ne-cryopreservation engaphantsi kwe-15 ° C kwiinyanga ezi-6.
.Zonke iisampulu zinqanda imijikelo yokunyibilika komkhenkce.

INKQUBO YOVAVANYO
Inkqubo yovavanyo yesixhobo bona incwadana ye-immunoanalyzer. Inkqubo yovavanyo lwe-reagent ngolu hlobo lulandelayo
I-1.Lay eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokungena kwi-akhawunti ngokwendlela yokusebenza yesixhobo, kwaye ungenise ujongano lokubona.
3.Skena ikhowudi yokuchaza ukuze uqinisekise into yovavanyo.
3.Khupha ikhadi lovavanyo kwibhegi yefoyile.
4.Faka ikhadi lovavanyo kwi-slot yekhadi, scan ikhowudi ye-QR, kwaye unqume into yovavanyo.
5.Yongeza i-30μL serum okanye isampuli yeplasma kwisisombululo, kwaye udibanise kakuhle.
6.Yongeza isisombululo se-20μL B kumxube ongentla, kwaye udibanise kakuhle.
Shiya umxube20imizuzu.
Yongeza umxube we-80μL kwisampulu yequla lekhadi.
Cofa iqhosha elithi "standard test", emva kwemizuzu eyi-10, isixhobo siya kubona ngokuzenzekelayo ikhadi lovavanyo, sinokufunda iziphumo kwisikrini sokubonisa sesixhobo, kwaye sirekhode / siprinte iziphumo zovavanyo.
Jonga kumyalelo we-Portable Immune Analyzer(WIZ-A101).

IZIPHUMO ZOMVAVANYO KUNYE UTOLIKO

.Le datha ingentla lithuba lereferensi elisekwe kwidatha yobhaqo yale khithi, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka iseke isithuba sesalathiso sokubaluleka kwezonyango olufanelekileyo lwabemi kulo mmandla.
.I-concentration ye-Estradiol iphezulu kunoluhlu lwereferensi, kwaye utshintsho lwe-physiological okanye impendulo yoxinzelelo kufuneka ingabandakanywa.Ngokuqinisekileyo ingaqhelekanga, kufuneka idibanise ukuxilongwa kweempawu zeklinikhi.
.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe yile ndlela, kwaye iziphumo azifani ngokuthe ngqo nezinye iindlela.
.Ezinye izinto zinokubangela iimpazamo kwiziphumo zokubona, kubandakanywa nezizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOZINZI
1.Ikhithi ziinyanga ezili-18 zobomi beshelufa ukusukela kumhla wokwenziwa. Gcina iikhithi ezingasetyenziswanga kwi-2-30°C. MUSA UKUMKHENZA. Musa ukusebenzisa ngaphaya komhla wokuphelelwa.
2.Musa ukuvula isingxobo esitywiniweyo de ube ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa olunye lucetyiswa ukuba lusetyenziswe phantsi kwendawo efunekayo (iqondo lobushushu 2-35℃, ukufuma 40-90%) ngaphakathi kwemizuzu engama-60 ngokukhawuleza. kangangoko kunokwenzeka.
I-3.I-diluent yesampuli isetyenziswa ngokukhawuleza emva kokuvulwa.

IZILUMKISO NEMIGAQO
.Ikhithi kufuneka itywinwe kwaye ikhuselwe kumswakama.
.Yonke imizekelo emihle iya kuqinisekiswa ngezinye iindlela.
.Yonke imizekelo iya kuphathwa njengongcoliseko olunokubakho.
.USUKU sebenzisa i-reagent ephelelwe lixesha.
. MUSA ukutshintshiselana ngeerejenti phakathi kweekhithi ezinenombolo eyahlukileyo.
.USUKU phinda usebenzise amakhadi ovavanyo kunye naziphi na izincedisi ezilahlwayo.
.I-Misoperation, isampuli eninzi okanye encinci inokukhokelela ekuphambukeni kweziphumo.

LUKUXELELA
.Njengalo naluphi na uvavanyo olusebenzisa izilwa-buhlungu zegundane, kunokwenzeka ukuba kukho ukuphazanyiswa yi-anti-mouse antibodies (HAMA) kwi-specimen. Iisampulu ezisuka kwizigulane ezifumene amalungiselelo ee-antibodies ze-monoclonal zokuxilongwa okanye unyango zingaquka i-HAMA. Imizekelo enjalo inokubangela ubuxoki okanye iziphumo ezingalunganga.
.Esi siphumo sovavanyo kuphela kwireferensi yeklinikhi, akufanele isebenze njengesiseko kuphela sokuxilongwa kweklinikhi kunye nonyango, ulawulo lwezonyango lwezigulane kufuneka luqwalaselwe ngokubanzi kunye neempawu zalo,
.imbali yezonyango, olunye uviwo lwebhubhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziselwa kuphela iimvavanyo ze-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe nomchamo, njl.
IIMPAWU ZOKUSEBENZA

RIMIZEKELO
1.Hansen JH, et al.HAMA Ukuphazamiseka kunye ne-Murine Monoclonal Antibody-Based Immunoassays [J] .J ye-Clin Immunoassay, 1993, 16: 294-299.
2.Levinson SS.Ubume be-Heterophilic Antibodies kunye nendima kwi-Immunoassay Interference[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo kwiisimboli ezisetyenzisiweyo:

	In Vitro Diagnostic Medical Isixhobo
	Umenzi
	Gcina kwi-2-30 ℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKO
	Qhagamshelana neMiyalelo yokuSetyenziswa

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