UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-FOB antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-FOB antibody kunye ne-IgG yomvundla kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-FOB kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-FOB antibody, kwaye yenze umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa intsonkothile iphumelele ummandla wovavanyo, idityaniswa ne-anti-FOB yokugquma i-antibody, yenza into entsha. Ukuba i-negative, isampuli ayinayo i-hemoglobin yomntu okanye umxholo uphantsi, i-immune complexes eyaneleyo ayinakwenziwa. Akuyi kubakho umgca obomvu kwindawo yokufumanisa (T). Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo:

Nceda ufunde iphakheji efakwe phambi kovavanyo.

1.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.

2.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokukhawuleza kwisampuli kakuhle yekhadi kunye ne-dispette enikeziweyo. Emva koko qalisa isibali-xesha.

3.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eyi-15.