Umgaqo

I-membrane yesixhobo sovavanyo sidityaniswe ne-fob antidy kwingingqi yovavanyo kunye nebhokhwe i-antibibit ye-Igg anti yoMda kwingingqi yolawulo. I-pad ibuthathaka idityaniswe yi-fluorescence elenzelwe i-anti fob antin antict kunye ne-rabbit yeg ngangoko. Xa uvavanya isampulu elungileyo, ifob antign kwisampulu idibanayo kunye ne-fluorescence elenzelwe i-Anti fob anti yoMntu, kwaye umxube ugonyamelo. Phantsi kwesenzo se-igonockographromatographrography, ukuhamba okuntsonkothileyo kwicala lephepha lokufumana. Xa intsokothi idlula ingingqi yovavanyo, idityaniswa ne-anti fob yokuhamba i-antidy, intshabalalo entsha. Ukuba ayinanto, isampulu ayiqulaneli i-hemoglobin yomntu okanye umxholo uphantsi, amayeza aneleyo okhuselo alwenziwa. Akuyi kubakho mgca obomvu kwindawo yokuchonga (t). Umgca obomvu ngumgangatho omiselweyo ubonakala kwindawo yoLawulo loMgangatho (c) wokugweba ukuba kukho iisampulu ezaneleyo nokuba i-chromatography yenkqubo iqhelekile. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi wee-regentnts.

Inkqubo yovavanyo:

Nceda ufunde ukufaka iphakheji ngaphambi kovavanyo.

1.Keke ikhadi lovavanyo kwingxowa ye-foil, yibeke etafileni itafile kwaye uphawula.

2.Remove i-cap kwityhubhu yesampulu kwaye ulahle i-rocp entsha ye-Drop, yongeza amaconsi ama-3 (malunga ne-roul) akukho bubble ityhefu kwaye ngokuthe ngcembe ukuya kwisampuli yekhadi elinikezwe i-spatch. Emva koko qala ixesha.

3.Iziphumo kufuneka zifundwe ngaphakathi kwemizuzu eli-10 ukuya kweli-15, kwaye ayisebenzi emva kwemizuzu eli-15.