UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-antigen ye-microalbumin kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi yelebhile iqatywe nge-fluorescence ebhalwe microalbumin kunye nomvundla we-IgG kwangaphambili. Ukuba akukho albumin emchameni, i-anti-Alb ebhalwe ngegolide e-anti-Alb ebhalwe nge-monoclonal kwiphepha legolide le-colloidal iyakusebenza kwinwebu kunye nomchamo ukuya kumgca wobhaqo, kwaye idibanise ne-Alb-coated antigen kunye nebonakalayo. umgca. Kwaye umbala womgca umnyama kunombala womgca kwindawo yolawulo (C), esi sisiphumo esibi. Ukuba umchamo uqulethe i-albumin, baya kukhuphisana ne-Alb-coated antigen kwi-membrane ukubophelela kwiindawo ezinqamlekileyo ze-antibody kwi-colloidal yegolide ebhalwe nge-anti-Alb-ebhalwe i-monoclonal antibody. Njengoko inani le-albumin kumchamo landa, uvavanyo

Umbala womgca uya kuba lula kwaye ulula. Umxholo we-albumin kumchamo unokufunyanwa ngokulinganayo ngokuthelekisa indawo yokufumanisa (T) kunye nommandla wokulawula (C). Indawo yokulawula umgangatho (C) kunye nendawo yokubhekisela (R) kwikiti iya kuhlala ibonakala ngexesha lovavanyo, kwaye ayinanto yakwenza nobukho be-albhamuin yomchamo. Ummandla wolawulo (C) kunye nomgca we-reference (R) unokusetyenziswa njengesalathisi sokulawula umgangatho wangaphakathi wekiti.

Inkqubo yoVavanyo:

Nceda ufunde incwadi yokusebenza kwesixhobo kunye nokufakwa kwephakheji phambi kovavanyo. Thaw iisampuli kwiqondo lobushushu begumbi phambi kokusetyenziswa.

1.Khupha ikhadi lovavanyo kwingxowa yefoyile. Yibeke emcaba kwindawo ethe tye kwaye uphawule.

2.Thatha isampuli yomchamo ngepipette elahlayo, ulahle amaconsi amabini okuqala esampuli yomchamo. Yongeza amathontsi ama-3 (malunga ne-100uL) omchamo ongenamaqamza kumbindi wesampulu yomngxuma wekhadi lovavanyo ngokuthe nkqo kwaye uqalise ixesha.

3.Funda umphumo kwimizuzu eyi-10-15. Ayisebenzi ukuba ingaphezulu kwemizuzu eli-15.