Ikhithi yokufumana isifo se-triodioidine(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
Ikhithi yokufumana isifo se-Triodiodicane (Fluorescence Junossopromatographic) yi-FluoresCographic I-Sunossographine ye-TriochicalOgraphicay ye-Triodoographine ye-Trioudomprographine ye-Triodoid ye-Trionection. kufuneka iqinisekiswe ngezinye iindlela zokusebenza. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
I-Triodioidymine (T3) Ubunzima bemolekyuli 651D. Yeyona ndlela iphambili yehomoni yehomoni ye-thyroid. Iyonke i-T3 (iyonke i-T3, i-T3) kwi-serum yahlulwe ukuba ibophelele kwaye ikhululekile. I-99.5% ye-TT3 ibophelele kwi-serm ye-proteroxine yokubopha (i-TBP), kunye ne-t3 t3 (i-t3) ye-0,2 ukuya kwi-0.4%. I-T4 kunye ne-T3 thatha inxaxheba ekugcineni nasekulawuleni umzimba we-metabolic yomzimba. I-TT3 yeKlinikhi yeKlinikhi ethembekileyo yokufumana i-hypelosism kunye ne-hyperthyroidism kunye ne-hypothyroidism.Imeko ye-T3 ibaluleke kakhulu ekufumaneni i-hypernidism kune-h4.

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo idityaniswe ne-Congemate ye-BSA kunye ne-T3 kummandla wovavanyo kunye nebhokhwe i-goat antibibit igubit antin anti yolawulo. I-padr pad idityaniswe yi-fluorescence marting anti t3 antibour kunye ne-rabit ye-Igg kwangaphambili. Xa isampulu yovavanyo, i-TT3 kwisampulu idibanisa i-fluorescence ephawulwe i-Anti T3 Antictur, kwaye iFouncy yomthwalo. Phantsi kwesenzo sesenzo se-igonocchiromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala le-Ancrictal, xa intsomi edlula i-T3 kwi-MAMRONERE i-T3 kwi-TT3 ye-TT3 ye-TT3 ye-TT3 ye-TT3 i Uxinzelelo lwe-TT3 kwisampulu inokufunyanwa yi-Fluorescence Jumossossay Assay.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
.I
.B Isisombululo se-1
I-A.Package Phot 1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay
Inkqubo yovavanyo yesixhobo ibona i-ismonananananananananananananananana yaseMunnoanananananalyzerzerzely. Inkqubo yovavanyo lokuvavanywa ngokulandelayo
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
6.Add 30μl kwi-serum okanye isampulu yeplasma kwisisombululo, kwaye uxube kakuhle.
I-7.ADD 20ADP 20 μl B kulo mxube ungasentla, kwaye uxube kakuhle.
8.Leave umxube kangangemizuzu engama-20.
9.Add 80μl umxube wesandla sekhadi.
10.Chemba "iqhosha lovavanyo oluqhelekileyo", emva kwemizuzu eli-10, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen sesixhobo, kwaye siprinte iziphumo.
11.Umfundi we-11.refer ukuya kumyalelo wohlalutyo obuphilayo ophathwayo (iWiz-A101).

Ixabiso elindelekileyo

I-TT3 Uluhlu oluqhelekileyo: 0.5-2.5ng / ml
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
. Idatha entla yedatha yethutyana yemboniselo yasekwa kwidatha efungukayo yale khit, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka iseke ithuba lokuphumla kwezonyango lwabantu.
.Ukugxininiswa kwe-TT3 iphezulu kune-Translations, kwaye utshintsho lonyango okanye impendulo yoxinzelelo mayibandakanywe. I-Abloed engaqhelekanga, kufuneka idibanise isifo seklinikhi.
.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi ezimiselwe yile ndlela, kwaye iziphumo azifani ngokuthelekise ezinye iindlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
1.Ikhi i-TIT i-18 leenyanga zeshelufa-ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2.Ungayivuli i-pouch etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwiMizuzu engama-60 ngokukhawuleza ngokusemandleni.
3.sample i-dilunguuent isetyenziswa kwangoko emva kokuvulwa.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

RIinkcazo
I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

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