I-TITOTIOTICTION CIT ye-thyroxine iyonke (I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
I-Kit yokufumana isifo se-thyroxine (Fluorescence I-I-Assunossographic) yi-FluoresComComCy I-AseRochic Sunoine kufuneka iqinisekiswe ngezinye iindlela zokusebenza. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
I-thyroxine (i-T4) ifihlwa yi-thyroid gland kunye nobunzima bayo molekyuli ngama-777d. Iyonke i-T4 (iyonke i-T4, i-TT4) kwi-serum ngamaxesha angama-50 e-serum t3. Phakathi kwabo, i-99.9% ye-TT4 Binds kwi-serm ye-proteroxine yokubopha (i-TBP), kunye ne-t4 ye-T4 (i-T4) ingaphantsi kwe-0.05%. I-T4 kunye ne-T3 ithatha inxaxheba ekulawuleni umsebenzi we-metabolic yomzimba. Imilinganiselo ye-TT4 isetyenziselwa ukuvavanya imeko kunye nokufumanisa izifo. Ikliniki, i-TT4 ingumqondiso onokuthenjwa wokuqonda kunye nokubonakalayo kwenkqubo ye-hypernidism kunye ne-hypothyroidism.

Umgaqo wenkqubo
I-membrane yesixhobo sovavanyo sidityaniswe ne-Congemate ye-BSA kunye ne-T4 kummandla wovavanyo kunye ne-goat anti yebhokhwe ye-antibibit igwebu. I-padker pad idityaniswe yi-Fluorescence marting anti t4 antibour kunye ne-rabibit ye-Igg kwangaphambili. Xa isampulu yovavanyo, i-TT4 kwisampulu idibanisa i-fluorescence ephawulwe i-Anti T4 Anti yoMntu, kwaye iBonelelo lokuQalisa. Phantsi kwesenzo sesenzo se-Ignoicndotographromatographromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala le-Ancrictal, xa intsomi edlula i-T4 kwi-MAMRONERE i-TT4 ye-TT4 ye-TT4 ye-TT4 ye-TT4 i Uxinzelelo lwe-TT4 kwisampulu inokufunyanwa yi-Fluorescence Jumossossay Assay.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
.I
.B Isisombululo se-1
I-A.Package Phot 1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay

Inkqubo yovavanyo yesixhobo ibona i-ismonananananananananananananananana yaseMunnoanananananalyzerzerzely. Inkqubo yovavanyo lokuvavanywa ngokulandelayo
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
3.Teke ikhadi lovavanyo kwingxowa ye-foil.
4.Unga iKhadi lovavanyo kwiKhadi leKhadi, Skena ikhowudi yeQR, kwaye ubone into yovavanyo.
I-SUMDMD 20μl i-serum okanye isampulu yeplasma kwisisombululo, kwaye ixube kakuhle.
6.Add 20ADP 20 μl B kulo mxube ungasentla, kwaye uxube kakuhle.

Shiya umxube we20imizuzu.
Yongeza i-80 yomxube kwi-Sala yekhadi lekhadi.
Cofa iqhosha elithi "Uvavanyo oluqhelekileyo", emva kwemizuzu eli-10, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, linokufunda iziphumo kwiscreen sescreen sesixhobo, kwaye siprinte iziphumo.
Bhekisa kumyalelo wohlalutyo ophathwayo komzimba ophathekayo (iWiz-A101).

Ixabiso elindelekileyo
Uluhlu lwe-TT4 Uluhlu oluqhelekileyo: 55-140nmol / l
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
. Idatha entla yedatha yethutyana yemboniselo yasekwa kwidatha efungukayo yale khit, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka iseke ithuba lokuphumla kwezonyango lwabantu.
.Ukugxininiswa kwe-TT4 iphezulu kunoluhlu lwereferensi, kwaye utshintsho lonyango okanye impendulo yoxinzelelo mayibandakanywe.
.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi ezimiselwe yile ndlela, kwaye iziphumo azifani ngokuthelekise ezinye iindlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
.Ikhithi yi-Thit yi-18 yeenyanga-ubomi-ubomi obuvela kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
.Ungavuleli i-pouch etywinwe ide ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwi-60 yemizuzu ngokukhawuleza kunokwenzeka.
.Ampleple isetyenziswe ngokukhawuleza emva kokuba ivuliwe.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

RIinkcazo
I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

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