Ikhithi yokuxilonga ye-Urine microalbumin

(Fluorescence Immunochromatographic Assay)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-Urine microalbumin (i-Fluorescence Immunochromatographic Assay) ifanelekile ukufumanisa ubuninzi be-microalbumin kumchamo womntu nge-fluorescence immunochromatographic assay, esetyenziselwa ukuxilongwa kwe-axiliary yesifo sezintso.Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO

I-Microalbumin yiprotheyini eqhelekileyo efumaneka egazini kwaye inqabile kakhulu emchameni xa isetyenzisiwe ngokwesiqhelo. Ukuba kukho umkhondo kumchamo Albumin ngaphezu kwama-20 micron / ml, yeyomchamo microalbumin, ukuba ngaba unyango olufike ngexesha, unako ukulungisa ngokupheleleyo glomeruli, ukuphelisa proteinuria, ukuba akukho unyango olufike ngexesha, unokungena kwisigaba uremia. I-microalbumin yomchamo ibonakala kakhulu kwi-nephropathy yesifo sikashukela, uxinzelelo lwegazi kunye ne-preeclampsia ekukhulelweni. Imeko inokuxilongwa ngokuchanekileyo ngexabiso le-urinary microalbumin, idibene nesiganeko, iimpawu kunye nembali yonyango. Ukufunyaniswa kwangoko kwe-microalbumin yomchamo kubaluleke kakhulu ukuthintela kunye nokulibazisa ukuphuhliswa kwe-nephropathy yesifo seswekile.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-antigen ye-ALB kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi yesiphawuli iqatywe nge-fluorescence uphawu lwe-anti-ALB kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu, i-ALB kwisampulu idibanisa kunye ne-fluorescence ephawulwe ukuba i-anti-ALB ye-antibody, kwaye yenze umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo, xa i-complex iphumelele ummandla wokuvavanya, I-marker ye-fluorescent yamahhala iya kudibaniswa ne-ALB kwi-membrane. Uxinzelelo lwe-ALB kwisampulu lunokubonwa ngovavanyo lwe-fluorescence immunoassay.

IIREGENTS KUNYE NEZINTO EZIBONISWAYO

Amacandelo ephakheji ye-25T:

Ikhadi lokuvavanya ifoyile nganye efakwe kwi-desiccant 25T

Faka ipakethi 1

IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA

Isampulu yokuqokelela isitya,ixesha

UKUQOKELELWA KWEsampuli NOKUGCINWA

1. Iisampulu ezivavanyiweyo zingangumchamo.
2. Iisampulu zomchamo omtsha zingaqokelelwa kwisitya esicocekileyo esilahlwayo. Kunconywa ukuvavanya iisampuli zomchamo ngokukhawuleza emva kokuqokelela. Ukuba iisampuli zomchamo azikwazi ukuvavanywa ngokukhawuleza, nceda uzigcine kwi-2-8℃, kodwa kucetyiswa ukuba ungagcinie kubo ngaphezu kweeyure ezili-12. Musa ukuyishukumisa isitya. Ukuba kukho intlenga ezantsi kwesikhongozeli, thatha i-supernatant ukuze uyivavanye.
3. Zonke iisampulu zinqanda imijikelo yokunyibilika komkhenkce.
4. Thaw iisampuli kwiqondo lobushushu begumbi phambi kokusetyenziswa.