Ikhithi yokufumana isifo kwi-chanalbumin

(I-Fluorescence I-Imbumbuographic Assay)

Kwi-vitro diagostistik kuphela

Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo

I-diginostic kit ye-Canalbumin (i-Fluorescence Junocromatographic) ilungele ukubonwa kwe-microAlbumic kwi-Aunoresbustographic Sunossion, esetyenziselwa isampulu ye-FluoresComy i-Aunoresness yesifo sezintso. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo

I-microalbumin yiproteyini eqhelekileyo efumaneka egazini kwaye inqabile kakhulu kumchamo xa i-metabolseled eqhelekileyo. Ukuba kukho isixa senani lomkhondo kwi-albhamun ye-albumin engaphezulu kwe-20 micreyalbumin, ukuba ingaba unyango lwe-ireminary i-iremerli, inokuthi igcine i-Glosurial Appralbuli, ikwandise i-Glosuri, ikwandise i-Glosternion enesifo seswekile. Le meko inokufunyanwa ngokuchanekileyo ngexabiso le-microalbumiry echasayo, idityaniswe nemeko, iimpawu kunye nembali yezonyango. Ukufunyanwa kwangoko kwe-microalbumin echasayo kubaluleke kakhulu ukuthintela kwaye kulibazise ukuphuculwa kweswekile neflebathy.

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo idityaniswe ne-Alb Antigen kummandla wovavanyo kunye nebhokhwe yebhokhwe i-Igg antin antin anti yolawulo. I-padker pad idityaniswe yi-Fluorescence Marko Anti Alking kunye ne-Igg ye-Igg ngaphambili. Xa isampulu yovavanyo, i-alb kwisampulu idibanisa i-fluorescence ephawulwe i-Anti Albuddy, kwaye ibunjwe ngamandla. Phantsi kwesenzo se-igonockographromatographromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala le-Arserting, i-sleoresting upapasho eliyi-Alb kwi-Ilbrane.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:

Ikhadi lovavanyo lokuqhekeka

Iphakheji ye-1

Izinto ezifunekayo kodwa azibonelelwanga

Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa

1. Iisampulu zavavanywa zinokuba ngumchamo.
2. Iisampulu zomchamo ezintsha zinokuqokelelwa kwisitya esicocekileyo esicocekileyo. Kucetyiswa ukuvavanya iisampulu zomchamo kwangoko emva kokuqokelela. Ukuba iisampulu zomchamo azinakuvavanywa kwangoko, nceda uzigcine nge-2-8℃, kodwa kuyacetyiswa ukuba ungayi sokoyazo ngaphezulu kweeyure ezili-12. Sukuqhubela isitya. Ukuba kukho i-sediment emazantsi esitya, thatha i-superntuant yovavanyo.
3. Yonke isampulu yokuphepha ukukhuluza imijikelezo ye-phandle.
4. Iisampulu zegumbi kubushushu begumbi ngaphambi kokusetyenziswa.