I-Diayistic Kit ye-isoenzyme MB ye-Dreatine Kinase(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
I-Diayistic Kit ye-isoenzyme MB ye-Dreayine Kinase (Fluorescence Junossophatroomatographic) kwiSluudotographic Mb ye-Osoen Kinocromatographic kwi-Ali I-infaction). Zonke isampulu eyakhayo kufuneka iqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
Amanqanaba e-CK-MB anyuke iiyure ezingama-4-6 emva kokuphelelwa yintsomi, ephethwe kwiiyure ezili-18 ukuya kwezi-24, wabuyela kwiintsuku ezingama-2-3 eziqhelekileyo kamva. Ukukhutshwa kwe-CK-MB ekujikelezeni emva kweHemoglobin.ck-MB ngumphawu ongummangaliso wesintu

Umgaqo wenkqubo
I-membrane yesixhobo sovavanyo idityaniswe ne-Anti CK-MB Antiy kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-anti I-pad ibuthathaka idityaniswe yi-fluorescence elenzelwe i-antic-MB antibir kunye ne-rabibit ye-Igg kwangaphambili. Xa uvavanya isampulu elungileyo, i-CK-MB Antigen kwisampulu idibanisa i-Fluorescence elenzelwe i-Anti CK-MB Anti yoMntu, kwaye iBonelela umxube. Phantsi kwesenzo se-Ignoicndotographromatographromatographromatographromatographromatographromapography, ukuhamba okuntsonkothileyo kwicala lephepha le-Ancrictant, xa intsomi egqithayo, idityaniswa ne-antic-Mb i-antidy anti youlelo, intshabalalo entsha. Inqanaba le-CK-MB lidibene kakuhle nomqondiso we-fluorescence, kwaye uxinzelelo lwe-CK-MB kwisampuli inokufunyanwa yi-Fluorescey Assossass.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
Ikhadi lovavanyo lokuqhekeka
Iisampulu zangaphantsi ze-25t
Iphakheji ye-1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay
Nceda ufunde isixhobo sokusebenza kwesixhobo kunye nokufakwa kwephakheji ngaphambi kovavanyo.
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
2.add 40μl i-serum okanye isampulu yeplasma kwisampuli yesampulu, kwaye xuba kakuhle ..
6.Add 80μl 80, isisombululo sesampulu kwisampulu yekhadi.
7.Clicke iqhosha elithi "Uvavanyo oluqhelekileyo", emva kwemizuzu eli-15, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen yesixhobo, kwaye siprinte iziphumo.
8.Refefer kumyalelo we-challocher yohlaluko ophathekayo (iWiz-A101).

Ixabiso elindelekileyo
Ck-mb <5ng / ml
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
. Idatha engasentla sisiphumo sovavanyo lwe-CK-MB Reagent, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka imisele uluhlu lwexabiso le-CK-MB lilungele ukubabekwa kwenani labantu abakule ndawo. Iziphumo ezilapha ngasentla zibhekiswa kuphela.
.Iziphumo zale ndlela zisebenza kuphela kwireferensi esekwe kule ndlela, kwaye akukho fani nye ngeendlela ngeendlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
1.Ikhi i-TIT i-18 leenyanga zeshelufa-ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2.Ungayivuli i-pouch etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwiMizuzu engama-60 ngokukhawuleza ngokusemandleni.
3.sample i-dilunguuent isetyenziswa kwangoko emva kokuvulwa.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

RIinkcazo
I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

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