Diagnostic Kit foriprotheni ye-hypersensitive C-reactive

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo kolu fakelo lwephakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-hypersensitive C-reactive protein (i-fluorescence immunochromatographic assay) luvavanyo lwe-fluorescence immunochromatographic yokufumanisa ubuninzi beprotheyini ye-C-reactive (CRP) kwi-serum yabantu / plasma/ Igazi elipheleleyo. Luphawu olungachanekanga lokudumba. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO

Iprotheyini ye-C-reactive yiprotheni yesigaba esibukhali eveliswa yi-lymphokine yokuvuselela isibindi kunye neeseli ze-epithelial. Ikhona kwi-serum yomntu, kwi-cerebrospinal fluid, pleural and abdominal fluid, njl. 6-8h emva kokuvela kosulelo lwebhaktheriya, i-CRP yaqala ukwanda, i-24-48h yafikelela kwincopho, kwaye ixabiso lencopho linokufikelela kumakhulu amaxesha aqhelekileyo. Emva kokupheliswa kosulelo, i-CRP yehla ngokukhawuleza kwaye yabuyela kwimeko yesiqhelo kwiveki enye. Nangona kunjalo, i-CRP ayinyuki kakhulu kwimeko yosulelo lwentsholongwane, olubonelela ngesiseko sokuchongwa kweentlobo zosulelo lokuqala lwezifo, kwaye sisixhobo sokuchonga usulelo lwentsholongwane okanye ibhaktheriya.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-CRP ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-CRP ye-antibody kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-CRP kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-CRP ye-antibody, kwaye yenze umxube wokuzivikela komzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-CRP yokwambathisa i-antibody, yenza into entsha. Inqanaba le-CRP lihambelana ngokufanelekileyo kunye nomqondiso we-fluorescence, kwaye ukuxinwa kwe-CRP kwisampulu kunokubonwa nge-fluorescence immunoassay assay.