Diagnostic Kit foriprotheyini ye-hypersensitive C-reactive

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-hypersensitive C-reactive protein (i-fluorescence immunochromatographic assay) luvavanyo lwe-fluorescence immunochromatographic yokufumanisa ubuninzi beprotheyini ye-C-reactive (CRP) kwi-serum yabantu / plasma/ Igazi elipheleleyo. Luphawu olungachanekanga lokudumba. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO

Iprotheyini ye-C-reactive yiprotheni yesigaba esibukhali eveliswa yi-lymphokine yokuvuselela isibindi kunye neeseli ze-epithelial. Ikhona kwi-serum yomntu, kwi-cerebrospinal fluid, pleural and abdominal fluid, njl. 6-8h emva kokuvela kosulelo lwebhaktheriya, i-CRP yaqala ukwanda, i-24-48h yafikelela kwincopho, kwaye ixabiso lencopho linokufikelela kumakhulu amaxesha aqhelekileyo. Emva kokupheliswa kosulelo, i-CRP yehla ngokukhawuleza kwaye yabuyela kwimeko yesiqhelo kwiveki enye. Nangona kunjalo, i-CRP ayinyuki kakhulu kwimeko yosulelo lwentsholongwane, olubonelela ngesiseko sokuchongwa kweentlobo zosulelo lokuqala lwezifo, kwaye sisixhobo sokuchonga usulelo lwentsholongwane okanye ibhaktheriya.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-CRP ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-CRP ye-antibody kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-CRP kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-CRP ye-antibody, kwaye yenze umxube wokuzivikela komzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-CRP yokwambathisa i-antibody, yenza into entsha. Inqanaba le-CRP lihambelana ngokufanelekileyo kunye nomqondiso we-fluorescence, kwaye ukuxinwa kwe-CRP kwisampulu kunokubonwa nge-fluorescence immunoassay assay.