I-diagostic kit ye-Estradiol(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
I-Diaclostic Kit ye-ESTRADOLL kufuneka iqinisekiswe ngezinye iindlela zokusebenza. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
I-Estradiol (E2) yeyona i-hormone ebaluleke kakhulu kwaye isebenza kakhulu kwi-folecrogen. I-E2 igcinwe ikakhulu yi-placenta, ngelixa amadoda eveliswa ikakhulu ngamaThesti. I-albhamun, imelolize evela kwisibindi kwi-Sulpiates enyibilikayo yamanzi okanye i-gluconaldehydes, kwaye ikhutshiwe evela kumchamo.e2 sisibonisi esibalulekileyo sehomoni yokuvavanya umsebenzi we-ovari.

Umgaqo wenkqubo
I-Mbrane yesixhobo sovavanyo idityaniswe ne-Congegicate ye-BSA kunye ne-ESTRADOL kwingingqi yovavanyo kunye nebhokhwe yebhokhwe ye-antibibit igubi. I-padker pad idityaniswe yi-Fluorescence Marko Anti E2 Antictur kunye ne-Antibibit Igg kwangaphambili. Xa isampulu yovavanyo, e2 kwisampulu idibanisa i-fluorescence ephawulwe i-Anti E2 Anti yoMntu, kwaye iFounction. Phantsi kwesenzo sesenzo se-igonocchromatographromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala le-Actorictal, xa intsomi egqithayo i-ESTradiol kwi-memradiol kwi-Estradiol ye-Estradiol ye-Fluores, kunye ne Uxinzelelo lwe-Estradiol kwisampuli inokufunyanwa yi-Fluorescence Junossossay Assay Assay.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
.I
.B Isisombululo se-1
I-A.Package Phot 1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
.Umzekelo uphephe ukuphepha imijikelezo ye-phandle.

Inkqubo ye-Assay
Inkqubo yovavanyo yesixhobo ibona i-ismonananananananananananananananana yaseMunnoanananananalyzerzerzely. Inkqubo yovavanyo lokuvavanywa ngokulandelayo
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
3.Teke ikhadi lovavanyo kwingxowa ye-foil.
4.Unga iKhadi lovavanyo kwiKhadi leKhadi, Skena ikhowudi yeQR, kwaye ubone into yovavanyo.
I-Stoum okanye isampulu yeplasma kwisisombululo, kwaye ixube kakuhle.
6.Add 20ADP 20 μl B kulo mxube ungasentla, kwaye uxube kakuhle.
Shiya umxube we20imizuzu.
Yongeza i-80 yomxube kwi-Sala yekhadi lekhadi.
Cofa iqhosha elithi "Uvavanyo oluqhelekileyo", emva kwemizuzu eli-10, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, linokufunda iziphumo kwiscreen sescreen sesixhobo, kwaye siprinte iziphumo.
Bhekisa kumyalelo wohlalutyo ophathwayo komzimba ophathekayo (iWiz-A101).

Iziphumo zovavanyo kunye notoliko

. Idatha entla yedatha yethutyana yemboniselo yasekwa kwidatha efungukayo yale khit, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka iseke ithuba lokuphumla kwezonyango lwabantu.
.Ukugxininiswa kwe-Estradiol kuphezulu kunoluhlu lwereferensi, kwaye utshintsho lonyango okanye impendulo yoxinzelelo mayibe ngaphandle kwengqondo.
.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi ezimiselwe yile ndlela, kwaye iziphumo azifani ngokuthelekise ezinye iindlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
1.Ikhi i-TIT i-18 leenyanga zeshelufa-ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2.Ungayivuli i-pouch etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwiMizuzu engama-60 ngokukhawuleza ngokusemandleni.
3.sample i-dilunguuent isetyenziswa kwangoko emva kokuvulwa.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo loKliniki kuphela, akufuneki lusebenze kuphela ngesiseko sokufumanisa isifo sekliniki kunye nonyango, ulawulo lweklinikhi yezigulana kufuneka luthathelwe ingqalelo ludityaniswe neempawu zalo,
Imbali ye-Imerical, olunye uviwo lwelebhu, impendulo yonyango, ityhudiyo kunye nolunye ulwazi.
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.
Iimpawu zentsebenzo

RIinkcazo
I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

Xiamen bio biotech co., Ltd
Idilesi:
I-TEL: + 86-592-6808278
Ifeksi: + 86-592-6808279