Ikhithi ye-dixisticI-Socirprotectin(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
I-Diaclostic Kit ye-Collitetin (Cal) ilungele ukumiselwa kobungakanani be-cluorestographic i-sunorespographic i-assolmay, enesampulu yefonti yefonti ye-biolmatic) kufuneka iqinisekiswe kwezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
I-CAL yi-heterodimer, edityaniswe yi-MRP 8 kunye ne-MRP 14^[1]. Ikhona kwi-neutrophils cytoplasm kwaye ibonakaliswe kwi-mononuclear yeseli. I-CAL ziiproteni zeSigaba seSigaba seAcutio, inesigaba esizinzileyo malunga neveki kwi-faeves yomntu, sizimisele ukuba sisifo sokungaziphathi kakuhle^[2-3]. Ikhithi luvavanyo olulula, olubonakalayo olubonakalayo olubona i-cal kumagumbi angabantu, inemvakalelo yokufumana imvakalelo kunye nobuchule obuqinileyo. Uvavanyo olusekwe kwicandelo eliphezulu le-antibodies ye-antibodies sandwich repite kunye ne-fluorescence i-I-Fluunoschromatographic Sciences

Umgaqo wenkqubo
I-Strip ine-anti-cal ontis antidy kwingingqi yovavanyo, efakwe kwi-membrane chromatographys kwangaphambili. I-padble ibuthathaka idityaniswe yi-fluorescence elenzelwe i-anti-cal antist. Xa uvavanya isampulu, i-cal kwisampuli inokuxubekilwa kunye ne-fluorescence elenzelwe i-anti-cal anticles, kwaye ibe ngumxube oqhelekileyo. Njengoko umxube uvumelekile ukuba afudukele kwi-Strip yovavanyo, i-cal cojegagicate strageging ifakiwe yi-anti-cal antid anti youlelo kwi-membrane kunye neefom ezinzima. Ubunzulu bobuqili buhambelana ngokuchanekileyo nomxholo we-CAL. I-CAL kwisampuli inokufunyanwa yi-Fluorescence Jumossey.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
Ikhadi lovavanyo lokuqhekeka
Iisampulu zangaphantsi ze-25t
Iphakheji ye-1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1. 1. Isikhongozeli esicocekileyo esincinci sokuqokelela isampuli entsha, kwaye ivavanywe kwangoko. Ukuba ayinakuvavanywa kwangoko, nceda ugcine nge-2-5 ° C kwiintsuku ezi-3 okanye ngezantsi -15 ° C kwiinyanga ezi-6.
2.Teke intonga yesampulu, ifakwe kwi-faeves isampulu yamaxesha ama-3, thatha indawo eyahlukeneyo ye-faeces, emva koko ubeka iSampulu, okanye usebenzise i-tick the ting, okanye usebenzisa i-Sampw Liny Ikhethiwe Malunga ne-50mg i-50mg i-faeves isampulu, kwaye ifakwe kwityhubhu yetyhubhu equkethe isimo sesampulu, kwaye i-screw ngokuqinileyo.
ISampung yePipette ilahleko ithatha i-faeces kwisampuli yesifo sohudo, emva koko yongeza amaconsi ama-3 (malunga ne-100μl) kwityhubhu ye-fecal kwaye inyikima kakuhle.

Amanqaku:
I-1.Avoid freeze-thaw thaws.
2.Kwie iisampulu kubushushu begumbi ngaphambi kokusetyenziswa.

Inkqubo ye-Assay

Nceda ufunde isixhobo sokusebenza kwesixhobo kunye nokufakwa kwephakheji ngaphambi kovavanyo.
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
6.Funda i-cap kwityhubhu yesampulu kwaye ulahle i-romp yokuqala ye-Drop, yongeza amaconsi ama-3 (malunga ne-roub) i-vericical ye-veritical kwaye ihamba kancinci kwi-vericeley ye-vericet.
7.Clicke iqhosha elithi "Uvavanyo oluqhelekileyo", emva kwemizuzu eli-15, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen yesixhobo, kwaye siprinte iziphumo.
8.Refefer kumyalelo we-challocher yohlaluko ophathekayo (iWiz-A101).

Ixabiso elindelekileyo
Cal <60μg / g
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
1.I-COCA kwisampulu ikwanjalo Iziphumo zingaqhelekanga kwaye kufuneka zifunyaniswe zineempawu zeklinikhi.
2.Iziphumo zale ndlela zisebenza kuphela kwireferensi esekwe kule ndlela, kwaye akukho fani nye ngeendlela ngeendlela.
3. Zonke izinto zinokubangela iimpazamo kwiziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
1.Ikhi i-TIT i-18 leenyanga zeshelufa-ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2.Ungayivuli i-pouch etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwiMizuzu engama-60 ngokukhawuleza ngokusemandleni.
3.sample i-dilunguuent isetyenziswa kwangoko emva kokuvulwa.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

Umda
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
. I-regent isetyenziselwa kuphela iimvavanyo zefecal. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

Izalathiso
I-1.li, g
2.n, W., Xu, jm, et al.I-ET ALDECY NECCERENTRIIN kwizigulana [J] .Cenase Joelis Colitis, ka-2010 (4)
3.wang, zh, i-guo, hb, et al.Isifundo malunga nobudlelwane be-fecal calamine kunye nesifo sethumbu. Itekhnoloji yeSayensi kunye noMphathiswa, ngo-2010-03,10 (8)

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

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