Diagnostic Kit forCalprotectin(Fluorescence Immunochromatographic Assay)
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO
Diagnostic Kit forCalprotectin(Cal) ilungele ukumiselwa kobungakanani belindle lomntu Cal nge-fluorescence immunochromatographic assay, ene-accessory ebalulekileyo ye-diagnostic value for inflammatory bowel disease.Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO
I-Cal yi-heterodimer, ehlanganiswe ne-MRP 8 kunye ne-MRP 14^[1]. Ikhona kwi-neutrophils cytoplasm kwaye ibonakaliswe kwi-mononuclear cell membranes. I-Cal yiprotheyini yesigaba esibukhali, inesigaba esizinzile malunga neveki enye kwilindle lomntu, izimisele ukuba ibe ngumqondiso wesifo sokukrala kwamathumbu.^[2-3]. Ikhithi luvavanyo olulula, olubonakalayo lobungakanani obufumanisa iCal kwilindle lomntu, inobuntununtunu obuphezulu bokubona kunye neenkcukacha ezinamandla. Uvavanyo olusekwe kwimilinganiselo ephezulu ye-antibodies ephindwe kabini kumgaqo wokuphendula isangweji kunye ne-fluorescence immunochromatographic assay assay technics, enokunika isiphumo kwimizuzu eli-15.

UMGAQO WENKQUBO
Umtya une-anti-Cal yokugquma i-antibody kwindawo yovavanyo, ebotshelelwa kwi-membrane chromatography kwangethuba. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-Cal antibody kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-Cal kwisampulu inokuxutywa kunye ne-fluorescence ebhalwe i-anti-Cal antibody, kwaye yenze umxube wokuzivikela komzimba. Njengoko umxube uvunyelwe ukuba ufuduke ecaleni komtya wovavanyo, i-Cal conjugate complex ibanjwa yi-anti-Cal yokugquma i-antibody kwi-membrane kwaye iifom ezintsonkothileyo. Ubunzulu befluorescence bunxulunyaniswa kakuhle nomxholo weCal. I-Cal kwisampulu inokubonwa nge-fluorescence immunoassay analyzer.

IIREGENTS KUNYE NEZINTO EZIBONISWAYO

Amacandelo ephakheji ye-25T:
Ikhadi lokuvavanya ifoyile nganye efakwe kwi-desiccant 25T
Isampulu yediluyinti 25T
Faka ipakethi 1

IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Isampulu yokuqokelela isitya,ixesha

UKUQOKELELWA KWEsampuli NOKUGCINWA
1.Sebenzisa isikhongozeli esicocekileyo esilahlwayo ukuqokelela isampulu yelindle elitsha, kwaye ivavanywe ngoko nangoko. Ukuba ayinakuvavanywa ngokukhawuleza, nceda igcinwe kwi-2-8 ° C iintsuku ezi-3 okanye ngaphantsi kwe-15 ° C kwiinyanga ezi-6.
2. Khupha intonga yesampulu, ifakwe kwisampulu yelindle, phinda isenzo izihlandlo ezi-3, thatha iindawo ezahlukeneyo zesampulu yelindle ixesha ngalinye, emva koko ubuyisele intonga yesampulu, ujike uqinise kakuhle, malunga nesampulu yelindle engama-50mg, kwaye ubeke kwityhubhu yesampulu yelindle equlethe isampulu yokuhlanjululwa, kwaye ujije ngokuqinileyo.
3.Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100µL) kwityhubhu yesampulu yelindle kwaye ungcangcazele kakuhle.

Amanqaku:
1.Kuphephe imijikelo yokunyibilika komkhenkce.
Iisampulu ze-2.Thaw kwiqondo lobushushu begumbi ngaphambi kokusetyenziswa.

INKQUBO YOVAVANYO

Nceda ufunde incwadi yokusebenza kwesixhobo kunye nokufakwa kwephakheji phambi kovavanyo.
I-1.Lay eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokungena kwi-akhawunti ngokwendlela yokusebenza yesixhobo, kwaye ungenise ujongano lokubona.
3.Skena ikhowudi yokuchaza ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwibhegi yefoyile.
5.Faka ikhadi lovavanyo kwi-slot yekhadi, scan ikhowudi ye-QR, kwaye unqume into yovavanyo.
6.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokucotha kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo.
7.Cofa iqhosha elithi "standard test", emva kwemizuzu eyi-15, isixhobo siya kubona ngokuzenzekelayo ikhadi lovavanyo, sinokufunda iziphumo kwisikrini sokubonisa isixhobo, kwaye sirekhode / siprinte iziphumo zovavanyo.
8.Jonga kumyalelo we-Portable Immune Analyzer (WIZ-A101).

IINDLELA EZILINDELEKILEYO
I-Cal <60μg/g
Kucetyiswa ukuba ilabhoratri nganye iseke uluhlu lwayo oluqhelekileyo olumele izigulane zayo.

IZIPHUMO ZOMVAVANYO KUNYE UTOLIKO
I-1.I-Cal kwisampuli ingaphezulu kwe-60μg / g, kwaye kufuneka ikhuphe utshintsho lwemeko ye-physiological. Iziphumo ngokwenene aziqhelekanga kwaye kufuneka zifunyanwe zineempawu zeklinikhi.
2.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe kule ndlela, kwaye akukho kuthelekiswa ngokuthe ngqo kunye nezinye iindlela.
I-3.Ezinye izinto zinokubangela iimpazamo kwiziphumo zokubona, kubandakanywa nezizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOZINZI
1.Ikhithi ziinyanga ezili-18 zobomi beshelufa ukusukela kumhla wokwenziwa. Gcina iikhithi ezingasetyenziswanga kwi-2-30°C. MUSA UKUMKHENZA. Musa ukusebenzisa ngaphaya komhla wokuphelelwa.
2.Musa ukuvula isingxobo esitywiniweyo de ube ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa olunye lucetyiswa ukuba lusetyenziswe phantsi kwendawo efunekayo (iqondo lobushushu 2-35℃, ukufuma 40-90%) ngaphakathi kwemizuzu engama-60 ngokukhawuleza. kangangoko kunokwenzeka.
I-3.I-diluent yesampuli isetyenziswa ngokukhawuleza emva kokuvulwa.

IZILUMKISO NEMIGAQO
.Ikhithi kufuneka itywinwe kwaye ikhuselwe kumswakama.
.Yonke imizekelo emihle iya kuqinisekiswa ngezinye iindlela.
.Yonke imizekelo iya kuphathwa njengongcoliseko olunokubakho.
.USUKU sebenzisa i-reagent ephelelwe lixesha.
. MUSA ukutshintshanisa iirejenti phakathi kweekhithi ezineqashiso ezahlukeneyo No..
.USUKU phinda usebenzise amakhadi ovavanyo kunye naziphi na izincedisi ezilahlwayo.
.I-Misoperation, isampuli eninzi okanye encinci inokukhokelela ekuphambukeni kweziphumo.

UMDA
.Njengalo naluphi na uvavanyo olusebenzisa izilwa-buhlungu zegundane, kunokwenzeka ukuba kukho ukuphazanyiswa yi-anti-mouse antibodies (HAMA) kwi-specimen. Iisampulu ezisuka kwizigulane ezifumene amalungiselelo ee-antibodies ze-monoclonal zokuxilongwa okanye unyango zingaquka i-HAMA. Imizekelo enjalo inokubangela ubuxoki okanye iziphumo ezingalunganga.
.Esi siphumo sovavanyo kuphela kwireferensi yeklinikhi, akufanele sisebenze njengesiseko kuphela sokuxilongwa kweklinikhi kunye nonyango, izigulane zolawulo lwezonyango kufuneka ziqwalaselwe ngokubanzi kunye neempawu zayo, imbali yonyango, ezinye iimviwo zebhubhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi. .
.Le sixhobo sisetyenziswa kuphela kuvavanyo lwe-fecal. Isenokungafumani ziphumo zichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe nomchamo, njl.

IIMPAWU ZOKUSEBENZA

IZIQINISEKISO
I-1.Li, G.&Y.L.Li.Ubudlelwane phakathi kwe-calcium kunye nesifo seklinikhi [J] .I-Journal ye-Practical Medicine, i-2007,23 (15)
2.Han, W., Xu, JM, et al. Uphononongo lweklinikhi lwe-calamine kunye ne-lactoferrin kwizigulane ezine-ulcerative colitis [J]. I-Chinese Journal ye-Gastroenterology, i-2010, i-15 (4)
3.Wang, ZH, Guo, HB, et al. Uphononongo malunga nobudlelwane phakathi kwe-fecal calamine kunye nesifo samathumbu esivuthayo[J]. Ubuchwephesha beNzululwazi nobuNjineli, 2010-03,10(8)

Isitshixo kwiisimboli ezisetyenzisiweyo:

	In Vitro Diagnostic Medical Isixhobo
	Umenzi
	Gcina kwi-2-30 ℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKO
	Qhagamshelana neMiyalelo yokuSetyenziswa

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