Ikhithi ye-dixisticIproteyini ye-cyperveritives c

(I-Fluorescence I-Imbumbuographic Assay)

Kwi-vitro diagostistik kuphela

Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo

I-Diaclostic Kit yeProtein ye-cypecial Chritives (Fluorescence Junoscopographic) yi-FluoresComCon I-FluUminic COUTOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOPROOTOPROOPROOPROOPROOPROOPROOPROOPROPROOTOOTOP) I-FLUOSCENTICROPROOPROOPROOTOPROOPROOPROOPROPROOTOOIC I-ASUTOKROPROPROPROPROPROOTOPROPROPROOTOOTOP EYENZIWAYO IPREATIC YOKUFUNDA NGOKUGQIBELA Luhlobo olungelulo lwe-insucasti. Zonke isampulu eyakhayo kufuneka iqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo

Iproteyini esebenzayo ye-C iproteyini yesigaba se-ACTEXE eveliswe ngu-Lymphokine wokuvuselela iiseli zesibindi kunye ne-epithelialal. Ikhona kwi-serum yomntu, ulwelo lwe-cerbrospinal, i-flaint kunye nesisu, kwaye iyinxalenye yendlela engalunganga yomzimba. I-6-8H emva kokwenzeka komsulwa webhacterial, i-CRP iqale ukunyuka, i-24-48h ifikelele kwi-piak, kwaye ixabiso le-peak linokufikelela kumaxesha aqhelekileyo. Emva kokupheliswa kosulelo, i-crp ihlise kabukhali kwaye ibuyele kwinto eqhelekileyo kwiveki enye. Nangona kunjalo, i-CRP ayinyuse kakhulu kwimeko yosulelo lwentsholongwane, olunikezela ngesiseko sokuchongwa kweentlobo zosulelo kwangoko kwezifo, kwaye sisixhobo sokuchonga usulelo lwentsholongwane okanye intsholongwane.

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo idityaniswe ne-Anti Crp Anti yoMmandla wovavanyo kunye nebhokhwe Anti Compabit Igg Anti yoMmandla woLawulo. I-pad ibuthathaka idityaniswe yi-fluorescence elenzelwe i-anti crp antid and kunye ne-rabibir ye-Igg kwangaphambili. Xa uvavanya isampulu elungileyo, i-crp antign kwisampulu indibaniselwano ye-Fluorescence elenzelwe i-Anti Crp Anti yoMntu, kwaye ibumbelele. Phantsi kwesenzo se-ignoindotographromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala lephepha le-Ancrictant, xa intsomi egqithayo iNgingqi, idityaniswe ne-Anti Carp One-Corp of Antidy, intshabalalo entsha. Inqanaba le-CRP lidibene kakuhle nomqondiso we-Fluorescence, kwaye uxinzelelo lwe-CRP kwisampulu inokufunyanwa yi-Fluorescence Assossass.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:

Ikhadi lovavanyo lokuqhekeka

Iisampulu zangaphantsi ze-25t

Iphakheji ye-1

Izinto ezifunekayo kodwa azibonelelwanga

Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa

1. Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-EDTA Anticoiclant iplasma.
2. Ngokweendlela ezisemgangathweni ziqokelela isampulu. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6. Isampulu yegazi lonke inokugcinwa kwi-2-8 ℃ kwiintsuku ezi-3
3. Yonke isampulu yokuphepha ukukhuluza imijikelezo ye-phandle.