I-Diagnostic Kit ye-Antigen ukuya kwi-Helicobacter Pylori(Fluorescence Immunochromatographic Assay)
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO
Diagnostic Kit for Antigen ukuya Helicobacter Pylori (Fluorescence Immunochromatographic Assay) ilungele ukubhaqwa kobungakanani ilindle lomntu HP antigen nge fluorescence immunochromatographic assay, nto leyo ebalulekileyo accessory ixabiso lokuxilonga usulelo esiswini. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO
Usulelo lwe-gastric helicobacter pylori lunxulumene ngokusondeleyo ne-gastritis engapheliyo, isilonda esiswini, i-adenocarcinoma yesisu, i-gastric mucosa ehambelana ne-lymphoma, izinga losulelo lwe-Hp ylori malunga ne-90% kwi-gastritis, i-gastric ulcer, i-duodenal ulcer kunye nezigulana zomhlaza wesisu. Umbutho wezempilo wehlabathi uchonge h. i-pylori njengohlobo lokuqala lomhlaza obangela umhlaza kwaye ngokucacileyo umngcipheko womhlaza wesisu.Ukufumanisa i-H.pylori kunexabiso elikhulu ekuxilongweni kwe-h. pylori infection.Uvavanyo olusekelwe kwi-fluorescence immunochromatographic assay analysis technics, enokunika umphumo kwimizuzu eyi-15.

UMGAQO WENKQUBO

Umtya une-anti-HP yokugquma i-antibody kwindawo yovavanyo, ebotshelelwa kwi-membrane chromatography kwangaphambili. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-HP antibody kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-HP kwisampulu inokuxutywa ne-fluorescence ebhalwe i-anti-HP antibody, kwaye yenze umxube wokugonyeka. Njengoko umxube uvumelekile ukuba ufuduke ecaleni komtya wovavanyo, i-HP conjugate complex ibanjwa yi-anti-HP yokugquma i-antibody kwi-membrane kwaye iifom ezintsonkothileyo. Ukuqina kwe-fluorescence kuhambelana kakuhle nomxholo we-HP. I-HP ekwisampulu inokubhaqwa nge-fluorescence immunoassay analyzer.

IIREGENTS KUNYE NEZINTO EZIBONISWAYO

Amacandelo ephakheji ye-25T:
Ikhadi lokuvavanya ifoyile nganye efakwe kwi-desiccant 25T
Isampulu yediluyinti 25T
Faka ipakethi 1

IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Isampulu yokuqokelela isitya,ixesha

UKUQOKELELWA KWEsampuli NOKUGCINWA
1.Sebenzisa isikhongozeli esicocekileyo esilahlwayo ukuqokelela isampulu yelindle elitsha, kwaye ivavanywe ngoko nangoko. Ukuba ayinakuvavanywa ngokukhawuleza, nceda igcinwe kwi-2-8 ° C iintsuku ezi-3 okanye ngaphantsi kwe-15 ° C kwiinyanga ezi-6.
2. Khupha intonga yesampulu, ifakwe kwisampulu yelindle, phinda isenzo izihlandlo ezi-3, thatha iindawo ezahlukeneyo zesampulu yelindle ixesha ngalinye, emva koko ubuyisele intonga yesampulu, ujike uqinise kakuhle, malunga nesampulu yelindle engama-50mg, kwaye ubeke kwityhubhu yesampulu yelindle equlethe isampulu yokuhlanjululwa, kwaye ujije ngokuqinileyo.
3.Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100µL) kwityhubhu yesampulu yelindle kwaye ungcangcazele kakuhle.

Amanqaku:
1.Kuphephe imijikelo yokunyibilika komkhenkce.
Iisampulu ze-2.Thaw kwiqondo lobushushu begumbi ngaphambi kokusetyenziswa.

INKQUBO YOVAVANYO
Nceda ufunde incwadi yokusebenza kwesixhobo kunye nokufakwa kwephakheji phambi kovavanyo.
I-1.Lay eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokungena kwi-akhawunti ngokwendlela yokusebenza yesixhobo, kwaye ungenise ujongano lokubona.
3.Skena ikhowudi yokuchaza ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwibhegi yefoyile.
5.Faka ikhadi lovavanyo kwi-slot yekhadi, scan ikhowudi ye-QR, kwaye unqume into yovavanyo.
6.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokucotha kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo.
7.Cofa iqhosha elithi "standard test", emva kwemizuzu eyi-15, isixhobo siya kubona ngokuzenzekelayo ikhadi lovavanyo, sinokufunda iziphumo kwisikrini sokubonisa isixhobo, kwaye sirekhode / siprinte iziphumo zovavanyo.
8.Jonga kumyalelo we-Portable Immune Analyzer (WIZ-A101).

IINDLELA EZILINDELEKILEYO
HP-Ag <10
Kucetyiswa ukuba ilabhoratri nganye iseke uluhlu lwayo oluqhelekileyo olumele izigulane zayo.

IZIPHUMO ZOMVAVANYO KUNYE UTOLIKO
1.I-HP-Ag kwisampulu ingaphezulu kwe-10, kwaye kufuneka ikhuphe utshintsho kwimeko ye-physiological. Iziphumo ngokwenene aziqhelekanga kwaye kufuneka zifunyanwe zineempawu zeklinikhi.
2.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe kule ndlela, kwaye akukho kuthelekiswa ngokuthe ngqo kunye nezinye iindlela.
I-3.Ezinye izinto zinokubangela iimpazamo kwiziphumo zokubona, kubandakanywa nezizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOZINZI
1.Ikhithi ziinyanga ezili-18 zobomi beshelufa ukusukela kumhla wokwenziwa. Gcina iikhithi ezingasetyenziswanga kwi-2-30°C. MUSA UKUMKHENZA. Musa ukusebenzisa ngaphaya komhla wokuphelelwa.
2.Musa ukuvula isingxobo esitywiniweyo de ube ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa olunye lucetyiswa ukuba lusetyenziswe phantsi kwendawo efunekayo (iqondo lobushushu 2-35℃, ukufuma 40-90%) ngaphakathi kwemizuzu engama-60 ngokukhawuleza. kangangoko kunokwenzeka.
I-3.I-diluent yesampuli isetyenziswa ngokukhawuleza emva kokuvulwa.

IZILUMKISO NEMIGAQO
.Ikhithi kufuneka itywinwe kwaye ikhuselwe kumswakama.
.Yonke imizekelo emihle iya kuqinisekiswa ngezinye iindlela.
.Yonke imizekelo iya kuphathwa njengongcoliseko olunokubakho.
.USUKU sebenzisa i-reagent ephelelwe lixesha.
. MUSA ukutshintshanisa iirejenti phakathi kweekhithi ezineqashiso ezahlukeneyo No..
.USUKU phinda usebenzise amakhadi ovavanyo kunye naziphi na izincedisi ezilahlwayo.
.I-Misoperation, isampuli eninzi okanye encinci inokukhokelela ekuphambukeni kweziphumo.

LUKUXELELA
.Njengalo naluphi na uvavanyo olusebenzisa izilwa-buhlungu zegundane, kunokwenzeka ukuba kukho ukuphazanyiswa yi-anti-mouse antibodies (HAMA) kwi-specimen. Iisampulu ezisuka kwizigulane ezifumene amalungiselelo ee-antibodies ze-monoclonal zokuxilongwa okanye unyango zingaquka i-HAMA. Imizekelo enjalo inokubangela ubuxoki okanye iziphumo ezingalunganga.
.Esi siphumo sovavanyo kuphela kwireferensi yeklinikhi, akufanele sisebenze njengesiseko kuphela sokuxilongwa kweklinikhi kunye nonyango, izigulane zolawulo lwezonyango kufuneka ziqwalaselwe ngokubanzi kunye neempawu zayo, imbali yonyango, ezinye iimviwo zebhubhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi. .
.Le sixhobo sisetyenziswa kuphela kuvavanyo lwe-fecal. Isenokungafumani ziphumo zichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe nomchamo, njl.

IIMPAWU ZOKUSEBENZA

RIMIZEKELO

I-1.Shao, JL & F.Wu. Inkqubela phambili yamva nje kwiindlela zokubona i-Helicobacter pylori [J] .I-Journal ye-Gastroenterology kunye ne-Hepatology, i-2012,21 (8): 691-694
2.Hansen JH, et al.HAMA Ukuphazamiseka kunye ne-Murine Monoclonal Antibody-Based Immunoassays [J] .J ye-Clin Immunoassay, 1993, 16: 294-299.
3.Levinson SS. Ubume be-Heterophilic Antibodies kunye nendima kwi-Immunoassay Interference [J].J ye-Clin Immunoassay, i-1992, 15: 108-114.

Isitshixo kwiisimboli ezisetyenzisiweyo:

	In Vitro Diagnostic Medical Isixhobo
	Umenzi
	Gcina kwi-2-30 ℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKO
	Qhagamshelana neMiyalelo yokuSetyenziswa

Xiamen Wiz Biotech CO.,LTD
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