I-Diaclostic Kit ye-Alpha-FetopOtein(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo

I-Diatostic Kiki ye-Alpha-Feotoscence I-I-Fluunochromatographic Assunossographic i-Alpha-Feungopotein (AFP) kwi-SUPOOPOCTROOP ye-Alpha-Fepatorial CarCoomar I-CarComa.all isampulu yakho kufuneka qinisekiswa ezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo

I-alpha-fetoprotein (AFP) yenye yeempawu eziqhelekileyo zethumba. ukuya kwi-100 mg / l; 1 ukuya kwele-2 emva kokuzalwa kwinqanaba labantu abadala; ukukhulelwa okuqhelekileyo kunokufikelela kwi-2 ukuya kwi-5 ukuya kwi-5 ukuya kwi-5 ukuya kwi-5 ukuya kwi-ML, kuchaphazela ixabiso le-AFP.

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo sidityaniswe nge-ANTI AFP Antids kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-anti I-pad ibuthathaka idityaniswe yi-fluorescence elenzelwe i-antif antin antiy kunye ne-rabibit yeg ngangoko. Xa uvavanya isampulu elungileyo, i-AFP antign kwisampulu idibanayo kunye ne-fluorescence elilebheli i-Antif Antiy, kwaye imbiseni umxube womzimba. Phantsi kwesenzo se-Ignoisotographromatographromatographromatographromatographrography, ukuhamba okuntsonkothileyo kwicala le-Arnoresces, idityaniswa ne-AFP edibeneyo, kunye nenqanaba le-AFP lidibene ne-AFP e-AFP, kwaye i-AFP yesampulu inokufunyanwa yi-fluorescey Assass.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:

.Beka ikhadi lokuqhekeka
I-.suples dileents 25t
I-A.Package Phot 1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha
Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay
Nceda ufunde isixhobo sokusebenza kwesixhobo kunye nokufakwa kwephakheji ngaphambi kovavanyo.
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
6.Add 20μl 20μl 20 μl i-serum okanye isampulu yeplasma kwisampuli yesampulu, kwaye udibanise kakuhle ..
I-7.ADS 80μl isampulu yesisombululo kwisampulu yekhadi.
8.Cota "iqhosha lovavanyo oluqhelekileyo", emva kwemizuzu eli-15, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen yesixhobo, kwaye siprinte iziphumo.
9.Refer ukuya kumyalelo we-challocher yohlalutyo obuqhelekileyo (iWiz-A101).

Ixabiso elindelekileyo

I-AFP: <10ng / ml
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
. Idatha entla sisiphumo sovavanyo lwe-Afp Rement, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka imisele uluhlu lweenqobo zokuchonga i-AFP ezifanelekileyo kubemi kule ndawo. Iziphumo ezilapha ngasentla zibhekiswa kuphela.
.Iziphumo zale ndlela zisebenza kuphela kwireferensi esekwe kule ndlela, kwaye akukho fani nye ngeendlela ngeendlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
1.Ikhi i-TIT i-18 leenyanga zeshelufa-ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2.Ungayivuli i-pouch etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (ubushushu 2-35 ℃, umswakama 40-90% kangangoko kunokwenzeka.
3.sample i-dilunguuent isetyenziswa kwangoko emva kokuvulwa.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
. Hayi regenges regeents phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
.I-Countral Vices kuphela kwireferensi yekliniki, akufuneki isebenze njengokuphela kwesifo sonyango kunye nonyango, ulawulo lwekliniki yezigulana kufuneka luthathe ulwazi oludityanisiweyo oludityanisiweyo ludityaniswa neempawu zalo, imbali yezonyango, impendulo yonyango, i-pidemiology kunye nolunye ulwazi.
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

RIinkcazo
I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

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