I-Diaclostic Kit ye-25-Hydroxy Vitamin D(I-Fluorescence I-Imbumbuographic Assay)
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
I-Diaclostic Kit ye-25-Hydroxy Vitamin D (i-Fluorescence Junossophatroomatographic) yi-FluoresCographic i-25 ye-fluoresce. I-Vitamin D.it yindawo yokuxilongwa nge-Auxilihansi yokuphinda ifunyanwe Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela.

Isishwankathelo
IVitamin D yivithamini kwaye ikwayihomoni ye-steroid, ikakhulu kubandakanya i-VD2 kunye ne-VD3, eyakhiwayo ifana kakhulu. IVitamin D3 kwaye i-D2 iguqulwe ibe yi-25 hydroxyl vithamin d (kubandakanya i-25-Dihydroxyl vithamin D3 kunye d2). 25- (oh) i-VD emzimbeni womntu, ukwakhiwa kwezinzile, uxinzelelo oluphezulu. 25- (oh) I-VD ibonakalisa inani lilonke leVitamin D, kunye ne-VION D, ukuze i-25- (o) i-VD ithathwa njengeyona nto ibalulekileyo yokuvavanya inqanaba leVitamin D.Iik I-ImmunocCology kwaye inokunika isiphumo ngaphakathi kwemizuzu eli-15.

Umgaqo wenkqubo
I-membrane yesixhobo sovavanyo idityaniswe ne-Congemate ye-BSA kunye ne-25- (o) i-VD kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-antibibit igwebu. I-padker pad idityaniswe yi-Fluorescence Mark Anti 25- (oh) i-vd anti) kunye ne-rabibit yeg ngangoko. Xa isampulu yovavanyo, i-25- (o) i-VD kwisampulu idibanisa i-fluorescence ephawulwe i-Anti 25- (oh) i-vd anti), kwaye ibe ngumxube womzimba. Phantsi kwesenzo se-Ignoicndotographromatographromatographromatographrography, ukuhamba ngokuntsonkothileyo kwicala le-Ancrictant, xa intsomi egqithayo i-25- (o) i-25- (oh) i-25- (o) I-VD imbi kwi-contrerating engalunganga yokubonisa umqondiso we-Fluorescence, kunye nokuxinana kwe-25- (oh) I-VD kwisampuli inokufunyanwa yi-fluorescey Assossass.

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
.I
.B Isisombululo se-1
I-A.Package Phot 1

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-serum okanye isampulu yeplasma inokugcinwa i-2-8 ℃ kwiintsuku ezisi-7 kunye ne-cryprest engezantsi -15 ° C kwiinyanga ezi-6.
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay
Inkqubo yovavanyo yesixhobo ibona i-ismonananananananananananananananana yaseMunnoanananananalyzerzerzely. Inkqubo yovavanyo lokuvavanywa ngokulandelayo
1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
6.Add 30μl kwi-serum okanye isampulu yeplasma kwisisombululo, kwaye uxube kakuhle.
I-7.ADD 8ADD 4 μl B kulo mxube ungasentla, kwaye uxube kakuhle.
8.Shiya umxube kangangemizuzu eli-15.
9.Add 80μl umxube wesandla sekhadi.
10.Chemba "iqhosha lovavanyo oluqhelekileyo", emva kwemizuzu eli-10, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen sesixhobo, kwaye siprinte iziphumo.
11.Umfundi we-11.refer ukuya kumyalelo wohlalutyo obuphilayo ophathwayo (iWiz-A101).

Ixabiso elindelekileyo
25- (oh) Uluhlu oluqhelekileyo lwe-VD: 30-100ng / ml
Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko
. Idatha entla yedatha yethutyana yemboniselo yasekwa kwidatha efungukayo yale khit, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka iseke ithuba lokuphumla kwezonyango lwabantu.
.Ukugxininiswa kwe-25- (oh) i-VD ingaphezulu kunoluhlu lwereferensi, kwaye utshintsho lonyango okanye impendulo yoxinzelelo mayibe ngaphandle kwengqondo.
.Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi ezimiselwe yile ndlela, kwaye iziphumo azifani ngokuthelekise ezinye iindlela.
. Izinto ezinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza
.Ikhithi yi-Thit yi-18 yeenyanga-ubomi-ubomi obuvela kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
.Ungavuleli i-pouch etywinwe ide ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwi-60 yemizuzu ngokukhawuleza kunokwenzeka.
.Ampleple isetyenziswe ngokukhawuleza emva kokuba ivuliwe.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Imifanekiso iya kuphathwa njengokungcola okunokubakho.
.Ungasebenzisi ukwenziwa okuphelelwe lixesha.
.Singeyiyo i-regeents yokuphinda-phinda phakathi kwe-kits enezinto ezahlukeneyo hayi ..
.Ungayisebenzisi kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
I-.miculation, isampulu egqithisileyo okanye encinci inokukhokelela kukuphambuka.

LUkuxelisa
.Awayo nayiphi na i-antibodies esebenzisa ii-antibodies zemouse, ukubakho malunga nokuphazamiseka zii-antibodies zemouse zabantu (i-hama) kwi-starimen. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

RIinkcazo

I-1.Ansen JH, i-ET Al.hama ingenelelo ngeMurne Monoclonional Antimy-esekwe kwi-Mymossassings [J] .UJEKO LEC UmgounOssasy, 1993,16: 294-299.
2.Levinson ss.Umntu we-antibophilic antibodies kunye nendima ekuphazamiseni kuka-InOssossis [J] .JO yeCRONAASSY NORONOASY, 1992,114.

Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa

Xiamen bio biotech co., Ltd
Idilesi:
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