UMGAQO

I-membrane yesixhobo sokuvavanya ifakwe kwi-conjugate ye-BSA kunye ne-25- (OH) VD kwindawo yokuvavanya kunye nebhokhwe yokulwa nomvundla we-IgG kwingingqi yokulawula. Iphedi yokumakisha iqatywe nge-fluorescence mark anti-25-(OH) VD antibody kunye nomvundla IgG kwangaphambili. Xa uvavanyo lwesampulu, 25-(OH) VD kwisampulu dibanisa fluorescence iphawulwe anti 25-(OH) VD antibody, kwaye wenze umxube omzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elifunxayo, xa i-complex idlula ummandla wokuvavanya, I-marker ye-fluorescent yamahhala iya kudibaniswa ne-25-(OH) VD kwi-membrane.Uxinzelelo lwe-25-(OH) I-VD lulungelelwaniso olubi lwesignali ye-fluorescence, kunye noxinaniso lwe-25-(OH) VD kwisampulu lunokubonwa nge-fluorescence immunoassay assay.

Inkqubo yoVavanyo:

Nceda ufunde incwadi yokusebenza kwesixhobo kunye nokufakwa kwephakheji phambi kovavanyo.

1. Beka eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokungena kwi-akhawunti ngokwendlela yokusebenza kwesixhobo, kwaye faka ujongano lokubona.
3. Skena ikhowudi yokuchaza ukuze uqinisekise into yovavanyo.
4. Khupha ikhadi lovavanyo kwibhegi yefoyile.
5. Faka ikhadi lovavanyo kwi-slot yekhadi, skena ikhowudi ye-QR, kwaye unqume into yovavanyo.
6. Yongeza i-15μL serum okanye isampuli yeplasma kwisisombululo, kwaye udibanise kakuhle
7. Yongeza umxube we-80μL kwisampulu yequla lekhadi.
8. Klikha iqhosha elithi "standard test", emva kwemizuzu eyi-15, isixhobo siya kubona ngokuzenzekelayo ikhadi lovavanyo, singakwazi ukufunda iziphumo kwiscreen esiboniswayo sesixhobo, kwaye sirekhode/siprinte iziphumo zovavanyo.
9. Jonga kumyalelo we-Portable Immune Analyzer (WIZ-A101).