IKit yokuxilongwa (LATEX) yeRotavirus Group A
Diagnostic Kit(LATEX)yeRotavirus Group A
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo kolu fakelo lwephakheji.
UKUSETYENZISWA OKUHLOSIWEYO
I-Diagnostic Kit (LATEX) ye-Rotavirus yeQela A ilungele ukufunyaniswa ngokomgangatho we-antigen ye-Rotavirus yeQela A kwiisampulu zelindle lomntu. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela. Ngeli xesha, olu vavanyo lusetyenziselwa ukuxilongwa kweklinikhi yohudo lomntwana kwizigulane ezinosulelo lwe-Rotavirus Group A.
UBUKHULU BOKUPACKAGE
Ikhithi eyi-1/ibhokisi, iikhithi ezili-10/ibhokisi, iikiti ezingama-25,/ibhokisi, iikiti ezingama-50/ibhokisi.
USHWANKATHELO
I-Rotavirus ihlelwa njengei-rotavirusuhlobo lwentsholongwane ye-exenteral, enemilo engqukuva enobubanzi obumalunga ne-70nm. I-Rotavirus iqulethe i-11 yamacandelo e-RNA ephindwe kabini. Ii-rotavirusingaba ngamaqela asixhenxe (ag) asekelwe kumahluko we-antigenic kunye neempawu zofuzo. Usulelo lwabantu lweqela A, iqela B kunye neqela C le-rotavirus liye labikwa. I-Rotavirus Group A ngunobangela obalulekileyo we-gastroenteritis enzima ebantwaneni kwihlabathi jikelele[1-2].
INKQUBO YOVAVANYO
1.Thatha intonga yesampulu, uyifake kwisampulu yelindle, emva koko ubeke intonga yesampulu ngasemva, jija uqinise kakuhle, phinda intshukumo ka-3. Okanye usebenzisa intonga yesampulu ichola malunga ne-50mg yesampuli yelindle, kwaye ufake kwityhubhu yesampulu yelindle equlethe isampulu yokuhlanjululwa, kwaye ujije ngokuqinileyo.
2.Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elibhityileyo kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu ye-fecal kwaye uvuthulule kakuhle, ubeke ecaleni.
3.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.
4.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokukhawuleza kwisampuli kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.
5.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eyi-15.