Diagnostic Kit(LATEX）yeRotavirus Group A
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO
I-Diagnostic Kit (LATEX) ye-Rotavirus yeQela A ilungele ukufunyaniswa ngokomgangatho we-antigen ye-Rotavirus yeQela A kwiisampulu zelindle lomntu. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela. Ngeli xesha, olu vavanyo lusetyenziselwa ukuxilongwa kweklinikhi yohudo lomntwana kwizigulane ezinosulelo lwe-Rotavirus Group A.

UBUKHULU BOKUPACKAGE
Ikhithi eyi-1/ibhokisi, iikhithi ezili-10/ibhokisi, iikiti ezingama-25,/ibhokisi, iikiti ezingama-50/ibhokisi.

USHWANKATHELO
I-Rotavirus ihlelwa njengei-rotavirusuhlobo lwentsholongwane ye-exenteral, enemilo engqukuva enobubanzi obumalunga ne-70nm. I-Rotavirus iqulethe i-11 yamacandelo e-RNA ephindwe kabini. I-rotavirus ingaba ngamaqela asixhenxe (ag) ngokusekelwe kwiiyantlukwano ze-antigenic kunye neempawu zofuzo. Usulelo lwabantu lweqela A, iqela B kunye neqela C le-rotavirus liye labikwa. I-Rotavirus Group A ngunobangela obalulekileyo we-gastroenteritis enzima ebantwaneni kwihlabathi jikelele^[1-2].

INKQUBO YOVAVANYO
1.Thatha intonga yesampulu, uyifake kwisampulu yelindle, emva koko ubeke intonga yesampulu ngasemva, jija uqinise kakuhle, phinda intshukumo ka-3. Okanye usebenzisa intonga yesampulu ichola malunga ne-50mg yesampuli yelindle, kwaye ufake kwityhubhu yesampulu yelindle equlethe isampulu yedilution, kwaye ujije ngokuqinileyo.
2.Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elibhityileyo kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu ye-fecal kwaye uvuthulule kakuhle, ubeke ecaleni.
3.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.
4.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokukhawuleza kwisampuli kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.
5.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eyi-15.