Ikhithi yokuqonda(IGolloidal igolide)ye-hormone yehomoni
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo

Ikhithi isetyenziselwa ukubongwa komgangatho we-Luteining Wormone (LH) amanqanaba kwisampulu yountu. Ilungele ukucacisa ixesha le-ovulation. Khokela abafazi bobudala bokuzala ukuze bakhethe ixesha elifanelekileyo lokukhulelwa, okanye ukukhokela i-tendoption ekhuselekileyo Zonke isampulu eyakhayo kufuneka iqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela. Ngeli xesha, olu vavanyo lusetyenziselwa i-IVD, izixhobo ezongezelelweyo azidingeki.

Ubungakanani bephakeji

I-1 Kit / ibhokisi yebhokisi ye-10 / ibhokisi ye-10, i-sokhithi ezingama-25, / ibhokisi, i-100 kits / ibhokisi.

Isishwankathelo
I-LH yi-gelycone ye-phycone efihliweyo yi-pituitary gland, ikho egazini lomntu kunye nomchamo, onokukhuthaza ukukhutshwa kwamaqanda aqolileyo kwi-agy. I-LH ifihliwe ngexesha lokuya esikhathini, kunye ne-LH Peak, ikhawuleza ikhutshiwe kwi-25-2 miu / ml ukusuka kwinqanaba elisisiseko le-5 ukuya kwi-Miu / ML. I-LH gxini kumchamo ihlala ikhula ngeyure ezingama-36 ukuya kwengama-148 ngaphambi kokuba i-ovulation, ipikisi kwiiyure ezili-14-28. Isixa se-LH kumchamo esidla ngokuqhubela phambili malunga ne-36 ukuya kwi-48 kwiiyure ezingama-14 ukuya kwi-14 ~ iiyure ezingama-28, i-membrane ye-ficur evuthiweyo emva kwe-pik kwaye yakhupha amaqanda aqolileyo. Abasetyhini bachume kakhulu kwi-LH Peak kwiintsuku ezili-1-3, ke, ukufumanisa i-LH emvukeni kunokusetyenziselwa ukuqikelela ixesha le-ovation^[1]. Le khise isekwe kwi-Colloidal Gold Gold Chromatographyty yetekhnoloji yohlalutyo lokufumana i-Antign ye-Antign kwiisampulu zomntu, ezinokunika isiphumo ngaphakathi kwemizuzu eli-15.

Inkqubo ye-Assay
1.Keke ikhadi lovavanyo kwingxowa ye-foil, yibeke etafileni itafile kwaye uphawula.
2.discard isampulu yokuqala ye-Drops, yongeza amaconsi ama-3 (malunga ne-100μl) akukho fubble ye-veriticaly kwaye icotha i-veriticaly kwaye ihamba kancinci ibe yindawo yekhadi elinikezelweyo, qala ixesha elinikezelweyo.
3.Iziphumo kufuneka zifundwe ngaphakathi kwemizuzu eli-10 ukuya kweli-15, kwaye ayisebenzi emva kwemizuzu eli-15.