Diagnostic Kit(Igolide yeColloidal）yeCalprotectin
Ukusetyenziswa kwe-in vitro diagnostic kuphela
Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO
I-Diagnostic Kit ye-Calprotectin (cal) luvavanyo lwe-colloidal yegolide ye-immunochromatographic yokuqinisekiswa kwe-semiquantitative ye-cal evela kwilindle lomntu, enexabiso elibalulekileyo lokuxilonga isifo sokukrala kwamathumbu. Olu vavanyo luyi-reagent yokuhlola. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela. Okwangoku, olu vavanyo lusetyenziselwa i-IVD, izixhobo ezongezelelweyo azidingeki.

USHWANKATHELO
I-Cal i-heterodimer, ehlanganiswe ne-MRP 8 kunye ne-MRP 14. Ikhona kwi-neutrophils cytoplasm kwaye ibonakaliswe kwi-mononuclear cell membranes. I-Cal i-acute phase proteins, inesigaba esizinzile kakuhle malunga neveki enye kwilindle lomntu, izimisele ukuba ibe yinto ephawulekayo yesifo sesibindi. Ikhithi luvavanyo olulula, olubonakalayo lwe-semiqualitative olufumanisa i-cal kwilindle lomntu, inovakalelo oluphezulu lokubona kunye neenkcukacha ezinamandla. Uvavanyo olusekwe kumgaqo-nkqubo wokuphendula wesangweji ye-antibodies ephindwe kabini kunye negolide ye-immunochromatographic assay, inokunika iziphumo kwimizuzu eli-15.

UMGAQO WENKQUBO
Umtya une-anti cal coating iMcAb kwindawo yovavanyo kunye nebhokhwe yokuchasa imivundla ye-IgG kwingingqi yolawulo, ebotshelelwa kwi-membrane chromatography kwangaphambili. Iphedi elayibhile iqatywe ngegolide ecolloidal ebhalwe anti cal McAb kunye negolide eyicolloidal ebhalwe i-antibody yomvundla we-IgG kwangaphambili. Xa uvavanya isampulu eqinisekileyo, i-cal kwisampulu yesampulu edibene negolide ye-colloidal ebhalwe anti cal McAb, kwaye yenza i-immune complex, njengoko ivunyelwe ukuba ifudukele ecaleni komtya wovavanyo, i-cal conjugate complex ibanjwa yi-anti cal coating McAb kwi-membrane kunye nefom. I-“anti cal coating iMcAb-cal-colloidal gold ebhalwe anti cal McAb” complex, ibhendi yovavanyo enemibala yavela kwindawo yovavanyo. Ukuqina kombala kuhambelana kakuhle nomxholo we-cal. Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Kungakhathaliseki ukuba i-cal ikhona kwisampulu okanye ayikho, kukho umgca obomvu ovela kummandla wereferensi kunye nommandla wolawulo lomgangatho, othathwa njengomgangatho wemigangatho yeshishini langaphakathi.

IIREGENTS KUNYE NEZINTO EZIBONISWAYO
Amacandelo ephakheji ye-25T:
.Ikhadi lovavanyo lwefoyile nganye efakwe kwi-desiccant
.Iisampulu zokuxutywa: izithako yi-20mM pH7.4PBS
.Disette
.Fakela ipakethe

IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA

Isampulu yokuqokelela isitya, isibali-xesha

UKUQOKELELWA KWEsampuli NOKUGCINWA
Sebenzisa isikhongozeli esicocekileyo esilahlwayo ukuqokelela isampuli yelindle elitsha, kwaye uvavanywe ngoko nangoko. Ukuba ayinakuvavanywa ngokukhawuleza, nceda igcinwe kwi-2-8 ° C kwiiyure ze-12 okanye i-bellow -15 ° C kwiinyanga ezi-4.

INKQUBO YOVAVANYO
1.Thatha intonga yesampulu, uyifake kwisampulu yelindle, emva koko ubeke intonga yesampulu ngasemva, jija uqinise kakuhle, phinda intshukumo ka-3. Okanye kusetyenziswa isampulu yesampulu echongwe malunga ne-50mg yesampuli yelindle, kwaye ifake kwityhubhu yesampulu yelindle enesampulu yokuhlanjululwa, kwaye ujije ngokuqinileyo.
2.Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elibhityileyo kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu ye-fecal kwaye uvuthulule kakuhle, ubeke ecaleni.
3.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.
4.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokukhawuleza kwisampuli kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.
5.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eyi-15.

IZIPHUMO ZOMVAVANYO KUNYE UTOLIKO

UKUGCINWA NOZINZI
Ikhithi ziinyanga ezingama-24 zobomi beshelufa ukusukela kumhla wokwenziwa kwayo. Gcina iikhithi ezingasetyenziswanga kwi-2-30°C. Musa ukuyivula isingxobo esivaliweyo de ube ukulungele ukwenza uvavanyo.
IZILUMKISO NEMIGAQO
1.Ikiti kufuneka itywinwe kwaye ikhuselwe kumswakama¹.
2.Ungasebenzisi isampulu ebekwe ixesha elide kakhulu okanye iphinde ikhenkceke kwaye inyibilike ukuze ivavanywe
3.Iisampulu zeFecal zigqithise okanye ubukhulu bunokwenza iisampuli ezihlanjululweyo zovavanyo olubi, nceda u-centrifuge isampuli ehlanjululweyo kwaye uthathe i-supernatant yokuvavanya.
I-4.I-Misoperation, isampuli eninzi okanye encinci ingakhokelela ekuphambukeni kweziphumo.

UMDA
1. Esi siphumo sovavanyo sesereferensi yeklinikhi kuphela, akufuneki sisebenze njengesiseko sokuxilongwa kweklinikhi kunye nonyango, ulawulo lwezonyango lwezigulane kufuneka luqwalaselwe ngokubanzi kunye neempawu zalo, imbali yezonyango, olunye uviwo lwebhubhoratri, impendulo yonyango, i-epidemiology kunye nezinye. ulwazi².
2.Le reagent isetyenziselwa kuphela iimvavanyo ze-fecal. Isenokungafumani ziphumo zichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe nomchamo, njl.