I-Diaclostic Kit Baysen

I-Diaclostic Kit

Inkcazo emfutshane:

Ixesha lovavanyo:Imizuzu eli-10-15

Ixesha elifanelekileyo:Inyanga engama-24

I-Accuranity:Ngaphezulu kwe-99%

Ukucaciswa:I-1/6 yovavanyo / ibhokisi

Iqondo lokugcina:2 ℃ -30 ℃

Thumela i-imeyile kuthi Khuphela njenge-PDF

Iinkcukacha zemveliso

Iimpawu zeMveliso

Ikhithi yokuqonda(IGolloidal igolide)yeyomlo
Kwi-vitro diagostistik kuphela
Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo
Ikhithi yokufumana isifo kwi-Collitectin (Cal) yi-Colloidal Goldromatographic Stures Ukuzimisela kwe-cal kwi-cal kwi-faeves, enesifo esibalulekileyo seForolostic kwi-FIFEALMAIT. Olu vavanyo yindawo yokufunda. Zonke isampulu eyakhayo kufuneka iqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela. Ngeli xesha, olu vavanyo lusetyenziselwa i-IVD, izixhobo ezongezelelweyo azidingeki.

Isishwankathelo
I-CAL yi-heterodimer, edityaniswe yi-MRP 8 kunye ne-MRP 14. Ikhona kwi-neutrophils cytophysm kwaye ibonakaliswe kwi-mononuclear yeseli. I-CAL ziiproteni zeSigaba seSigaba seAcutio, inesigaba esizinzileyo malunga neveki kwi-faeves yomntu, sizimisele ukuba uphawu lwe-biolmatimes. Ikhithi iluvavanyo olulula, olubonakalayo lwe-semiqualialitive olufumanayo lubona amathafa oluntu, ineemvakalelo eziphezulu zobuntu kunye nengqondo enamandla. Uvavanyo olusekwe kwi-antiboding ephezulu ye-antibodies sandwich reamwich reamwich reamwich reamwich reewwich kunye neGenocromatographic Sturesgraphics, inokunika isiphumo ngaphakathi kwemizuzu eli-15.

Umgaqo wenkqubo
I-Strip ine-anti caric carating MCAB kwingingqi yovavanyo kunye nebhokhwe Anti-umvundla i-Igg Anti yolawulo, efakwe kwi-membrane chromatographys kwangaphambili. I-pad ibuthathaka idityaniswe yiColoidal yeGolide ye-Anti cal Mcab kunye ne-Colloidal yeGolide yeLebhu ye-Igg Xa uvavanya isampulu elungileyo, i-cal kwisampuli ene-Colloidal yegolide eneelebhu, i-forvidal yegolide ye-MCAB ifakwe kwi-Anti Crating Mcab "I-Anti Central, ibhendi yovavanyo olunemibala kwingingqi yovavanyo. Ubunzulu bemibala buhambelana kakuhle nomxholo we-CAL. Isampulu engalunganga ayivelisi i-quand yovavanyo ngenxa yokungabikho kwegolide yeGolide Cougegiate Cal. I-CACE ifumaneka kwisampuli okanye hayi, kukho umtya obomvu kuvela kumda wesalathiso kunye nengingqi yolawulo lwemigangatho, ethathwa njengemigangatho yeshishini yangaphakathi.

I-Regeents kunye nezixhobo ezibonelelweyo
I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
Ii-Dilients ezinqabileyo: Izithako ziyi-20mm ph7.4pbs
.Diskette
. Faka

Izinto ezifunekayo kodwa azibonelelwanga

Isikhongozelo sokuqokelela isampula, ixesha

Ukuqokelelwa kwesampulu kunye nokugcinwa
Sebenzisa isikhongozeli esicocekileyo sokuqokelela isampuli entsha, kwaye uvavanywe kwangoko. Ukuba ayinakuvavanywa kwangoko, nceda ugcine nge-2-8 ° C kwi-12hours okanye i-bline -15 ° C kwiinyanga ezi-4.

Inkqubo ye-Assay
1.Teke intonga yesampulu, ifakwe kwi-faeves isampulu, emva koko ibeke umandlalo wesampulu, screw ngokuqinileyo kwaye inyibilika kakuhle, phinda amaxesha ama-3. Okanye usebenzisa isampulu-i-Sample enamanzi malunga ne-50mg i-50mg i-50mg ye-50mg ye-50mg ye-tueces, kwaye ifakwe kwi-theeces paipe ityhubhu equkethe isampula, kwaye i-screw ngokuqinileyo.
I-Funeuse iSampulte ye-Pipette ithatha i-faepen emdaka kwi-chees kwisigulana, emva koko yongeza amaconsi ama-3 (malunga ne-til) kwityhubhu ye-fecal kunye ne-shake kakuhle, ibeke ecaleni.
3.Teke ikhadi lovavanyo kwingxowa ye-foil, yibeke etafileni kwaye uyiphawule.
4.Remove i-cap kwityhubhu yesampulu kwaye ulahle i-romp yesibini ye-Drop, yongeza amaconsi ama-3 (malunga ne-roul) i-vericical ye-veritical kwaye ihamba kancinci kwi-vericete, ixesha lokuqalisa ixesha elinikezelweyo.
Isiphumo kufuneka sifundwe ngaphakathi kwemizuzu eli-10 ukuya kweli-15, kwaye ayisebenzi emva kwemizuzu eli-15.

Iziphumo zovavanyo kunye notoliko

	Iziphumo zovavanyo	Ingcaciso
①	I-Red Referet Band kunye ne-bandapper yolawulo ebomvu kwingingqi ye-R kunye ne-COD, akukho bomvuIbhendi yovavanyo kwingingqi ye-T.	Kuthetha umxholo we-faecescalprotelin yomntu iphantsi kwe-15μg / g, eyiyoinqanaba eliqhelekileyo.
②	I-Red Referet Band kunye ne-bandapper ebomvu kwingingqi ye-R kunye ne-COD, kunye neUmbala we-Red Reference Ibhendi imnyama kuneIbhendi yovavanyo ebomvu.	Umxholo we-Poeustin Poivesprotectin i-IbetIn yeBitbekwe phakathi kwe-15μ kunye ne-60μ0g / g. Inokuba njaloKwinqanaba eliqhelekileyo, okanye kunokubakho umngcipheko weIsifo se-syndrome esicaphukileyo.

③	I-Red Referet Band kunye ne-bandapper ebomvu kwingingqi ye-R kunye ne-COD, kunye neUmbala webhendi ebomvu yereferensi iyafanaIbhendi yovavanyo ebomvu.	Umxholo we-Forprotectin yoMntu i-Compotectin yi -60μg / g, kwaye kukho umngcipheko ofanelekileyo weIsifo se-bimolmatimes.
④	I-Red Referet Band kunye ne-bandapper ebomvu kwingingqi ye-R kunye ne-COD, kunye neUmbala webhendi yovavanyo olubomvu lumnyama kunobomvuIreferensi yereferensi.	Ibonisa umxholo we-faescalprotelin yomntu ingaphezulu kwe-60μ0 / g, kwaye aphoNgaba i-fansifan efikelela emngciphekweni we-flommatobisifo.
⑤	Ukuba ibhendi yereferensi ebomvu kunye nebhendi ebomvu engabonwayo okanye ibone nje enye, uvavanyoithathwa njengengasebenziyo.	Phinda uvavanyo usebenzisa ikhadi lovavanyo elitsha.

Ukugcinwa kunye nokuzinza
Ikhithi yiinyanga ezingama-24-ubomi-ubomi beenyanga ezingama-24 ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Sukuvula i-pouch etywiniweyo de ulungele ukwenza uvavanyo.
Izilumkiso kunye nezilumkiso
1.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma¹.
2.Ungasebenzisi isampulu ebekwe ixesha elide okanye iphindaphindwe kwaye iphinde ifundwe kunye nokuvavanywa
Iisampulu ze-3.ubsfeal zigqithisa okanye ubukhulu zinokwenza iisampulu ezixineneyo
4.Imifanekiso, isampulu egqithisileyo okanye encinci inokukhokelela ekuphambene.

Umda
I-1.Isiphumo sovavanyo lwekliniki kuphela kwisalathiso seklinikhi kuphela, akufuneki sisebenze njengokuphela kwesifo sekliniki kunye nonyango, ulawulo lwekliniki yezigulana kufuneka luthathe ulwazi oludityanisiweyo ludityaniswa neempawu zalo, imbali yezonyango, impendulo yonyango, i-pidemiology kunye nolunye ulwazi².
2.Le Dagent isetyenziselwa kuphela iimvavanyo zefecal. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Izalathiso
[1] INkqubo yovavanyo lweKlinikhi yeSizwe leKlinikhi (ihlelo lesithathu, ka-2006). ISebe lezeMpilo leMpilo.
[2] amanyathelo okulawula kubhaliso lwe-Vitro Digentnt. I-China yokutya nokutya, hayi. 5 Ucwangco, 2014-07-30.
Iqhosha lokuphatha elisetyenzisiweyo: