Ikhithi yokuxilonga yeProcalcitonin

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo kolu fakelo lwephakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-Procalcitonin (i-fluorescence immunochromatographic assay) yi-fluorescence immunochromatographic assay yokufumanisa ubuninzi be-Procalcitonin (PCT) kwi-serum yabantu okanye i-plasma, isetyenziselwa ukuxilongwa kwentsholongwane ye-bacterial and sepsis. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO

Iprocalcitonin yenziwe nge-116 amino acids kunye nobunzima bayo bemolekyuli yi-12.7KD. I-PCT ibonakaliswa ngamaseli e-neuroendocrine kwaye idilizwe yi-enzymes kwi-calcitonin (engakabikho) i-calcitonin, i-carboxy-terminating peptide, kunye ne-amino yokuphelisa i-peptide. Abantu abaphilileyo banomlinganiselo omncinci we-PCT egazini labo, onokunyuswa kakhulu emva kokusuleleka kwintsholongwane. Xa i-sepsis isenzeka emzimbeni, izicubu ezininzi zinokubonisa i-PCT, ngoko ke i-PCT ingasetyenziswa njengesalathiso se-sepsis. Kwezinye izigulane ezinosulelo oluvuthayo, i-PCT ingasetyenziselwa njengesalathisi sokukhethwa kwe-antibiotic kunye nesigwebo esisebenzayo.

UMGAQO WENKQUBO

Inwebu yesixhobo sovavanyo igqunywe nge-anti-PCT ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-PCT ye-antibody kunye ne-IgG yomvundla kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-PCT kwisampulu idibanisa kunye ne-fluorescence ebhalwe i-anti-PCT antibody, kwaye yenza umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula ummandla wovavanyo, idityaniswe ne-anti-PCT yokwambathisa i-antibody, yenza into entsha. Inqanaba le-PCT lihambelana ngokufanelekileyo kunye nesignali ye-fluorescence, kwaye ukuxinwa kwe-PCT kwisampuli kunokubonwa nge-fluorescence immunoassay assay.