Ikhithi yokuxilonga yeProcalcitonin

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kwe-in vitro diagnostic kuphela

Nceda ufunde le phakheji ifake ngononophelo phambi kokuba uyisebenzise kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho nakuphi na ukutenxa kwimiyalelo ekweli phakheji.

UKUSETYENZISWA OKUHLOSIWEYO

I-Diagnostic Kit ye-Procalcitonin (i-fluorescence immunochromatographic assay) yi-fluorescence immunochromatographic assay yokufumanisa ubuninzi be-Procalcitonin (PCT) kwi-serum yabantu okanye i-plasma, isetyenziselwa ukuxilongwa kwentsholongwane ye-bacterial and sepsis. Zonke iisampuli ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa kweengcali zezempilo kuphela.

USHWANKATHELO

Iprocalcitonin yenziwe nge-116 amino acids kunye nobunzima bayo bemolekyuli yi-12.7KD. I-PCT ibonakaliswa ngamaseli e-neuroendocrine kwaye idilizwe yi-enzymes kwi-calcitonin (engakabikho) i-calcitonin, i-carboxy-terminating peptide, kunye ne-amino yokuphelisa i-peptide. Abantu abaphilileyo banomlinganiselo omncinci we-PCT egazini labo, onokunyuswa kakhulu emva kokusuleleka yintsholongwane. Xa i-sepsis isenzeka emzimbeni, izicubu ezininzi zinokubonisa i-PCT, ngoko ke i-PCT ingasetyenziswa njengesalathiso se-sepsis. Kwezinye izigulane ezinosulelo oluvuthayo, i-PCT ingasetyenziselwa njengesalathisi sokukhethwa kwe-antibiotic kunye nesigwebo esisebenzayo.

UMGAQO WENKQUBO

Inwebu yesixhobo sovavanyo igqunywe nge-anti-PCT ye-antibody kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-PCT ye-antibody kunye ne-IgG yomvundla kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-PCT kwisampulu idibanisa kunye ne-fluorescence ebhalwe i-anti-PCT antibody, kwaye yenza umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula ummandla wovavanyo, idityaniswe ne-anti-PCT yokwambathisa i-antibody, yenza into entsha. Inqanaba le-PCT lihambelana ngokufanelekileyo kunye nesignali ye-fluorescence, kwaye ukuxinwa kwe-PCT kwisampuli kunokubonwa nge-fluorescence immunoassay assay.