UMGAQO

Inwebu yesixhobo sovavanyo igqunywe nge-antibody ye-Cpn-IgM kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti-Cpn-IgM ye-antibody kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-Cpn-IgM kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-Cpn-IgM ye-antibody, kwaye yenza umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-Cpn-IgM yokugquma i-antibody, yenza into entsha. Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo

Inkqubo yovavanyo lwe-WIZ-A202 kunye ne-Portable Immune analyzer WIZ-A101 ibhekisela kumyalelo womyalelo. Inkqubo yovavanyo olubonakalayo lulandelayo:

1. Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.
2. Yongeza i-10μl serum okanye isampulu yeplasma okanye i-20ul yonke isampuli yegazi ukuze isampula kakuhle yekhadi kunye ne-dispette enikeziweyo, uze udibanise i-100μl (malunga ne-2-3 yehla) isampuli ye-diluent; qalisa ixesha.
3. Lindela ubuncinci bemizuzu eyi-10-15 kwaye ufunde umphumo, umphumo awuvumelekile emva kwemizuzu eyi-15.