UMGAQO

Inwebu yesixhobo sovavanyo igqunywe nge-antibody ye-Tf kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti Tf antibody kunye nomvundla we-IgG kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo, i-antigen ye-Tf kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-Tf antibody, kwaye yenze umxube wokugonyeka. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-Tf yokugquma i-antibody, yenza into entsha.

Ukuba i-negative, isampuli ayinayo i-transferrin okanye umxholo uphantsi, ukwenzela ukuba i-immune complex ingenziwa. Akuyi kubakho umgca obomvu kwindawo yokufumanisa (T). Kungakhathaliseki ukuba i-antibody ye-Tf ikhona kumzekelo okanye ayikho, i-antibody ye-colloidal yegolide eseleyo ebhalwe ngegolide echasene nomntu ye-IgM ye-monoclonal kunye ne-anti-mouse ye-IgG yebhokhwe egqunywe kumgca wokulawula umgangatho (C). Emva koko i-agglutinates iphuhlisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kubonakala kwi (C). Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo

Nceda ufunde iphakheji efakwe phambi kovavanyo.

1.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.

2.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokukhawuleza kwisampuli kakuhle yekhadi kunye ne-dispette enikeziweyo. Emva koko qalisa isibali-xesha.