Umgaqo

I-membrane yesixhobo sovavanyo idityaniswe ne-tf antidy kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-anti I-pad ibuthathaka idityaniswe yi-Fluorescence elenzelwe i-anti tf antin kunye ne-rabibit igg kwangaphambili. Xa uvavanya isampulu elungileyo, i-Antign Antign kwiSampulu idibanisa i-Fluorescence elebed anti tf anti you antion, kwaye imbiseni umxube wokhuselo. Phantsi kwesenzo se-igonockographromatographrography, ukuhamba okuntsonkothileyo kwicala lephepha lokufumana. Xa intsokothi idlula ingingqi yovavanyo, idityaniswa ne-anti tf i-antidy anti youngs, intshabalalo entsha.

Ukuba ayinanto, isampulu ayiqulathanga i-sprin okanye umxholo uphantsi, ukuze kwenzeke into yokuba amajokweni omzimba ayinakwenziwa. Akuyi kubakho mgca obomvu kwindawo yokuchonga (t). Nokuba i-tf antiddy ikhona kwi-speciant okanye hayi, i-Count-i-Count-i-Ig ye-Igm ye-Anti-Gude Gom Monoclonion Antiy Anti yoMdibaniso we-Anti-Igmed Anti yoLawulo lweMgangathweni (c) umgca. Emva koko i-ageglutinates iphuhlisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kuvela (c). Umgca obomvu ngumgangatho omiselweyo ubonakala kwindawo yoLawulo loMgangatho (c) wokugweba ukuba kukho iisampulu ezaneleyo nokuba i-chromatography yenkqubo iqhelekile. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi wee-regentnts.

Inkqubo yovavanyo

Nceda ufunde ukufaka iphakheji ngaphambi kovavanyo.

1.Keke ikhadi lovavanyo kwingxowa ye-foil, yibeke etafileni itafile kwaye uphawula.

2.Remove i-cap kwityhubhu yesampulu kwaye ulahle i-rocp entsha ye-Drop, yongeza amaconsi ama-3 (malunga ne-roul) akukho bubble ityhefu kwaye ngokuthe ngcembe ukuya kwisampuli yekhadi elinikezwe i-spatch. Emva koko qala ixesha.