UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-anti AFP kwingingqi yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti AFP antibody kunye nomvundla we-IgG kwangaphambili. Xa kuvavanywa isampulu eqinisekileyo, i-antigen ye-AFP kwisampulu idibanisa ne-fluorescence ebhalwe i-anti-AFP antibody, kwaye yenze umxube wokugonyeka. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula ummandla wovavanyo, idityaniswe ne-anti-AFP yokwambathisa i-antibody, yenza inqanaba elitsha le-complex. kwisampulu inokubonwa ngovavanyo lwe-fluorescence immunoassay.

Inkqubo yoVavanyo

Nceda ufunde incwadi yokusebenza kwesixhobo kunye nokufakwa kwephakheji phambi kovavanyo.

1. Beka eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokungena kwi-akhawunti ngokwendlela yokusebenza kwesixhobo, kwaye faka ujongano lokubona.
3. Skena ikhowudi yokuchaza ukuze uqinisekise into yovavanyo.
4. Khupha ikhadi lovavanyo kwibhegi yefoyile.
5. Faka ikhadi lovavanyo kwi-slot yekhadi, skena ikhowudi ye-QR, kwaye unqume into yovavanyo.
6. Yongeza i-20μL serum okanye isampulu yeplasma kwisampulu yokuxutywa, kwaye udibanise kakuhle.
7. Yongeza i-80μL yesisombululo sesampulu kwisampulu yequla lekhadi.
8. Klikha iqhosha elithi "standard test", emva kwemizuzu eyi-15, isixhobo siya kubona ngokuzenzekelayo ikhadi lovavanyo, singakwazi ukufunda iziphumo kwiscreen sokubonisa sesixhobo, kwaye sirekhode/siprinte iziphumo zovavanyo.
9. Jonga kumyalelo we-Portable Immune Analyzer (WIZ-A101).