Sinandipha isimo esihle kakhulu phakathi kwethemba lethu lentengiso yethu enkulu yomgangatho ophezulu, ixabiso lokhuphiswano kunye nenkonzo efanelekileyo ye-8 i-China ichanekile ngaphandleI-HCV yovavanyo lwe-HCVIikhilomitha zovavanyo ezikhawulezayo kwizibonelelo zohlalutyo zePathoigical, besizithembile ukuba kuya kuthathwa njengelize kwaye siyathemba ukuba sinokuba nentsebenziswano yexesha elide ngamathemba okusingqongileyo.
Siyonwabela isimo esihle kakhulu phakathi kwamathemba ethu kwi-merchandise yethu enkulu, ixabiso lokhuphiswano kunye nenkonzo efanelekileyo yeUvavanyo olukhawulezileyo lwe-China, I-HCV yovavanyo lwe-HCV, Iimpahla zethu zithandwa kakhulu eLizwini, njengoMzantsi Merika, Africa, iAsia njalo njalo. Inkampani
Kwi-vitro diagostistik kuphela

Nceda ufunde le phakheji Faka ngononophelo ngaphambi kokusetyenziswa kwaye ulandele ngokungqongqo imiyalelo. Ukuthembeka kweziphumo ze-Assay akunakuqinisekiswa ukuba kukho nakuphi na ukuphambuka kwimiyalelo kule mpahla yephakheji.

Ukusetyenziswa kwenjongo

I-Diatistic Kit yeHipatitis C i-His Antium Antium Aduunographic Assunospographic) i-FluoSCographic Anticromatographic Anti Iindlela zokusebenza. Olu vavanyo lwenzelwe ukhathalelo lwempilo kuphela

1.Ukulinda ecaleni kwazo zonke ii-regents kunye neesampulu kubushushu begumbi.
2.Umntu ophantsi komzimba ophathwayo
3.Scan ikhowudi yokuqonda ukuqinisekisa into yovavanyo.
4.Teke ikhadi lovavanyo kwingxowa ye-foil.
5.Nika ikhadi lovavanyo kwikhadi le-slot, skena ikhowudi ye-QR, kwaye ubone into yovavanyo.
6.Add 20μl 20μl 20 μl i-serum okanye isampulu yeplasma kwisampuli yesampulu, kwaye udibanise kakuhle ..
I-7.ADS 80μl isampulu yesisombululo kwisampulu yekhadi.
8.Cota "iqhosha lovavanyo oluqhelekileyo", emva kwemizuzu eli-15, isixhobo siyakufumana ngokuzenzekelayo iKhadi loVavanyo, inokufunda iziphumo kwiscreen sescreen yesixhobo, kwaye siprinte iziphumo.
9.Refer ukuya kumyalelo we-challocher yohlalutyo obuqhelekileyo (iWiz-A101).

Isishwankathelo

I-Hepatitis C intsholongwane (i-HCV) yimvulophu, i-chiv engatshatanga ye-RNA (9.5 KB) intsholongwane yentsapho yaseFlavididae. I-genotypes ezintandathu kunye nothotho lwezityholo ze-HCV zichongiwe. I-Wenaved ngo-1989, i-HCV ngoku iqatshelwe njengonobangela ophambili wotofelo-mpahla onxulumene no-A, ongengo-B-B hepatitis. Esi sifo sibonakaliswa ngefom engapheliyo nengapheliyo. Ngaphezulu kwe-50% yabantu abosulelekileyo baphuhliswa kakhulu, ubomi bokusongela i-hepatiti engapheliyo ene-cirrhoisis yesibindi kunye ne-hepatocellas. Ukususela kwintshayelelo ngo-1990 ye-Anti-HCV yokuhlolwa kweminikelo yegazi, isimo sesulelo kuTransfusion incitshisiwe kakhulu. Izifundo zeklinikhi zibonisa ukuba inani elikhulu le-HCV labantu abosulelekileyo be-HCV bakhulisa ii-antibodies ukuya kwi-NS5 engeyiyo eyakhiwe yentsholongwane. Ngale nto, iimvavanyo zibandakanya i-antigens kwingingqi ye-NS5 ye-chinome yentsholongwane ukongeza kwi-NS3 (C200), NS4 (C200) kunye ne-core (C22).

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo idityaniswe ne-HCV Antigen kummandla wovavanyo kunye nebhokhwe yebhokhwe ye-antibibit igubi. I-padble ibuthathaka idityaniswe yi-fluorescence elebheli antign kunye ne-rabibit yeg ngangoko. Xa uvavanywa isampulu elungileyo, i-HCV Antids kwisampulu idibanisa i-Fluorescence elenzelwe i-HCV Antign, kunye nomxube ongumkhundleni. Phantsi kwesenzo semilinganiselo ye-igonockiromatographromatographromatography, ukuhamba ngokuntsonkothileyo kwicala le-Antigen, idityaniswa ne-HCV Antign, i-Ivale ye-Antigv Antign idityaniswe nomqondiso we-Fluores, kunye nokuxinana kwe I-HCV Antiddy kwisampuli inokufunyanwa yi-Fluorescence Junossassay Assay

I-Regeents kunye nezixhobo ezibonelelweyo

I-25T iphakheji yephakheji:
.Beka ikhadi lokuqhekeka
.Ample Dluents
. Faka

Izinto ezifunekayo kodwa azibonelelwanga
Isikhongozelo sokuqokelela isampula, ixesha
Ukuqokelelwa kwesampulu kunye nokugcinwa
1.Iza neesampulu ezivavanywayo zinokuba yi-serum, i-heparin anticoiculant yePlasma okanye i-Edta Anticoulant iplasma.
2.Ukuba ubuchwephesha obuqhelekileyo buqokelela isampula. I-Srium okanye isampulu yePlasma inokugcinwa kwi-2-8 ℃ kwiintsuku ezisi-7 kunye ne-crytoprest engezantsi -15 ° C kwiinyanga ezi-6
3.Nzulu uSam pawuni uphephe imijikelezo yenkunkuma.

Inkqubo ye-Assay
Nceda ufunde isixhobo sokusebenza kwesixhobo kunye nokufakwa kwephakheji ngaphambi kovavanyo.
. I-Colo loVavanyo loVavanyo lwekliniki kuphela, akufuneki lisebenze kuphela isiseko sokufumanisa isifo sekliniki kunye nonyango, imbali yezonyango, imbali yezonyango, impendulo yelebhu, iSandiso loNyango, iFidemiology kunye nolunye ulwazi .
.I-regent isetyenziselwa kuphela iimvavanyo zeserum kunye nePlasma. Isenokungafumaneki iziphumo ezichanekileyo xa isetyenziselwa ezinye iisampulu ezinje ngamathe kunye nomchamo kwaye njl.

Iimpawu zentsebenzo

Izalathiso
1.UThengiso lwe-hepatitis ye-hepatitis. Kwi: Moore sb, ed. I-transfusion-edluliselwa intsholongwane. I-Alington, VA. Am. I-ACHEC. Iibhanki zegazi, i-PP. 53-38.
I-Ansansen JH, i-ET Al.hama i-ET
3.Levinson SS.Uhlobo lwe-antibophilic antibodies kunye nendima ekuphazamiseni kukaJunsossassi [J] .U-1992,15: 108-114.
4.NXA HJ., Pucell rh, holland pv, et al. . I-Lancet I: 459-463.
5.Khoo ql, wener Aj, uTalby lr, Kuo G, i-Houghton M. (1990) Hepatitis C virus: Eyona Ages iphambili ye-Vipatitis engeyiyo i-vipatitis engeyiyo. Br med inkunzi yesi-46: 423-441.
6.Engvall E, Perlmann P. (1971) I-Enzyme idityaniswe kwi-Isnosorbent Assay. UMOLUNGU 8: 871-874.

Ixabiso elindelekileyo

I-HCV-AB <0.02

Kucetyiswa ukuba ilabhori nganye imisele uluhlu lwayo oluqhelekileyo olukwindawo yalo yesigulana.

Iziphumo zovavanyo kunye notoliko

• Le datha ingentla sisiphumo sovavanyo lwe-HCV-AB Recture, kwaye kucetyiswa ukuba ilabhoratri nganye kufuneka imisele uluhlu lwemilinganiselo ye-HCV-AB efanelekileyo kubemi abakwingingqi. Iziphumo ezilapha ngasentla zibhekiswa kuphela.
• Iziphumo zale ndlela zisebenza kuphela kwireferensi esekwe kule ndlela, kwaye akukho mpawu ngqo nezinye iindlela.
• Ezinye izinto zinokubangela iimpazamo ngokweziphumo zokuchonga, kubandakanya nezizathu zobugcisa, iimpazamo zomsebenzi kunye nezinye izinto zesampulu.

Ukugcinwa kunye nokuzinza

1. Ikhithi yi-18 yeenyanga zeSlufan-Ubomi ukusuka kumhla wokwenziwa. Gcina i-kits ezingasetyenziswanga nge-2-30 ° C. Musa ukukhulula. Sukusebenzisa ngaphaya komhla wokuphelelwa.
2. Musa ukuvula ibhedi etywiniweyo de ulungele ukwenza uvavanyo, kwaye uvavanyo lokusetyenziswa lunye lucetyiswa ukuba lusetyenziswe phantsi kwendalo esingqongileyo (iqondo lokushisa 2-35 ℃, umswakama 40-90) kwiMizuzu engama-60 ngokukhawuleza .
3. Isampulu isetyenziswe ngokukhawuleza emva kokuba ivuliwe.

Izilumkiso kunye nezilumkiso
.Ikhithi kufuneka itywinwe kwaye ikhuselwe nxamnye nokufuma.
.Khumbula ukuba iisampulu ezilungileyo ziya kuqinisekiswa zezinye iindlela.
.Zonke iimodeli ziya kuphathwa njengokungcola okunokubakho.
.Sukusebenzisa ukwenziwa okuphelelwe lixesha.
.Musa ukungahambelani kwakhona kwezinto ze-redages phakathi kweekhithi ezinezinto ezahlukeneyo ..
.Sukuyisebenzisa kwakhona amakhadi ovavanyo kunye nakuphi na izixhobo zokulahla.
.Ukusetyenziswa gwenxa, isampulu eninzi okanye encinci ingakhokelela ekuphazeni.

LUkuxelisa
.Njengakwi-antibodies esebenzisa ii-antibodies ze-antibodies zemouse, ukubakho malunga nokuphazamiseka kwi-antibodies ye-antibodies yomntu (i-hama) kwinqaku. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
Iqhosha lokuphatha elisetyenzisiweyo:

	Kwisixhobo sonyango lweVitrolostic
	Umvelisi
	Gcina nge-2-30 ℃
	Umhla WOKUPHELELWA
	SUKUFUMANA
	Isilumkiso
	Nxibelelana nemiyalelo yokusetyenziswa