Thrononot ea Thranosto bakeng sa Thranroxine e felletseng (fluorescence cluolumochromatographic
Bakeng sa li-vitro tsa ho etsa joalo
Ka kopo bala sephutheloana sena sa sephutheloana pele ho ka sebelisoa le ho latela litaelo. Ho ts'epahala ha liphetho tse tsoelang pele ho ke ke ha netefatsoa haeba ho na le maemo a tsoang ho litaelo tse kenyang sephutheloana sena.

Ts'ebeliso e reriloeng
KittySOTTIC Kit bakeng sa Thramroxine (TT4EMENCECOMICICICICICICICICICICICICICICICICICICICICS e tlameha ho netefatsoa ke mekhoa e meng. Teko ena e etselitsoe boits'oaro ba bophelo bo botle feela.

Kakaretso
Thyroxine (T4) e rometsoe ke tšoelesa ea qoqotho le boima ba eona ba limolek'hule ke 777D. Kakaretso ea T4 (kakaretso ea T4, TT4) Serum ke makhetlo a 50 ea serum t3. Har'a bona, 99.9% ea TT4 e thibela protheine ea Thyround (TLP), le mahala ea T4 (mahala t4 (mahala t4 T4 le T3 Kenya mosebetsi oa ho laola tšebetso ea 'mele ea' mele. Litekanyo tsa TT4 li sebelisetsoa ho lekola maemo a qoqotho le tšoaetso ea maloetse a qotsitsoeng a sebetsang. Ka tleleniki, TT4 ke sesupo se tšepahalang bakeng sa tlhahlobo le boits'oaro bo sebedlicory le hypothyroidism.

Molao-motheo oa Ts'ebetso
Membrane ea sesebelisoa sa tlhahlobo e koahetsoe ke conjugate ea BSA le T4 sebakeng sa liteko le poli antiboth sebakeng sa taolo. Marker Pad a koahetsoe ke letšoao la fluorescceccence Anti T4 antibody le rabbit t4 esale pele. Ha ho hlahloba sampole, TT4 ho sampole e le cluorescence e tšoaeang fluorescence e bonoang e anti T4 motsoako oa ho itšireletsa. Tlas'a ketso ea ho tšoaroa ke immunochundographmoy Khatello ea TT4 ka sampole e ka fumanoa ke fluorescence Cluosassay.

Reagents le lisebelisoa tse fanoeng

Mehloli ea sephutheloana sa 25t:
Card. Tilly ka foil e entsoe ka lesika la botsitso 25t
.A tharollo 25T
.B tharollo 1
.PaChelage e kenya 1

Lisebelisoa tse hlokahalang empa ha li fanoe
Mohlala oa mohlala oa mohlala, timer

Ho bokelloa ha mohlala le ho boloka
1.Ke lekoa ke semela e ka ba serum, Heparin Anticoagulant plasma kapa EDTARagulant plasma.
2.Cutcording ho isa mekhoa e tloaelehileng e bokella mehlala. Serum kapa Plasma samne ea plasma e ka bolokoa e haneloa ho 2-8 ℃ bakeng sa matsatsi a 7-5
Mohlala oa 3.All o qoba ho fafatsa molaetsa-thaw.

Ts'ebetso ea Assay

Ts'ebetso ea liteko ea sesebelisoa e bona bukana ea Iltrananglyzer. Ts'ebetso ea liteko e ratoang e ntse e latela
1.Lay ka thoko litsie le mekgatlo tsohle tsa mekgutshi.
2.O Sebui sa 'mele se keteketsi (Wiz-A101), kenya phasewete ea ak'haonte ho latela mokhoa oa ts'ebetso ea sesebelisoa,' me u kenye sebopeho sa ho lemoha sesebelisoa.
3.scan khoutu ea meno ho netefatsa ntho ea tlhahlobo.
3.Take ka karete ea liteko e tsoang ka mokotleng oa foil.
4. Ansert Card ea Teko ka karete ea karete, hlahloba khoutu ea QR, 'me u khetholle ntho ea tlhahlobo.
5.DD 20__l serum kapa sampole ea plasma ka tharollo, 'me u kopanya hantle.
6.ADDD 20μl B Tharo ho motsoako o ka pele, 'me o kopanya hantle.

Tlohela motsoako bakeng sa20metsotso.
Kenya motsoako 80μl motsoako ho ea mohlala hantle oa karete.
Tobetsa konopo ea "Standra Standard", kamora metsotso e 10, sesebelisoa se tla bala karete ea liteko, mme e rekota liphetho tsa tlhahlobo.
Sheba taeo ea sebui sa 'mele se ke keng sa hlahlojoa (Wiz-a101).

Melao e lebelletsoeng
Tt4 e tloaelehileng: 55-140NNmol / L
Ho khothalletsoa hore theborato e 'ngoe le e' ngoe e tiisa hore e tloaelehile e emela baahi ba eona ba bakuli.

Liphetho tsa liteko le toloko
.Tota data e kaholimo ho nako ea ho khetholloa bakeng sa data ena, 'me ho khothaletsoa hore mosebetsi o mong le o mong o ka theha nako ea palo ea bana sebakeng sena.
.Ka likhohlano tsa TT4 e phahame ho feta mookoto, 'me karabelo ea khatello ea maikutlo e lokela ho khethoa, e lokela ho kopana le motho ea sa tloaelehang.
.Thotso ea mokhoa ona e sebetsa feela ho ratoang ho ratoang ke mokhoa ona, 'me liphetho ha li tšoane ka kotloloho le mekhoa e meng ka kotloloho.
.

Ho boloka le ho tsitsa
.Ke kit ke sehatsetsi sa likhoeli tse 18 ho tloha ka letsatsi la tlhahiso. Boloka li-kitsi tse sa sebelisoeng ho 2-30 ° C. Se ke oa bata. Se ke oa sebelisa ntle le letsatsi la ho felloa ke nako.
.Do se ke oa bula mokotla o tiisitsoeng ho fihlela o se o loketse ho etsa liteko, 'me tlhahlobo e le' ngoe e lokela ho sebelisoa tlasa tikoloho ea 40-90%) kahare ho nako e 20 ho khoneha.
.Sumple e kopane hangata kamora ho buloa.

Litemoso le Litlhokomelo
.Ke ka ho ts'oaroa le ho sireletsoa mongobo.
Lipapatso tse ntle tse ntle li tla netefatsoa ke mekhoa e meng.
Lipapatso tsa .Le.
.Do e se e sebelisetsoa ho hlakisoa.
.Do e sa fetohe li-regels tsa li-kits tse nang le lotho e fapaneng.
.Do ho se sebelise likarete tsa liteko le lisebelisoa life kapa life tse lahlehileng.
.MoSisoperation, sampole e feteletseng kapa e nyane e ka lebisa ho kheloha.

LHo Etsisoa
.A ka assays e 'ngoe le e' ngoe ea li-antibodies, monyetla o teng ho li-antibodies tsa anti-mouse (Hama) ka mokhoabong. Mehlala ea bakuli ba fumaneng litokisetso tsa li-antibodies tsa menoclon bakeng sa tlhahlobo kapa kalafo e ka ba le hamma. Mehlala e joalo e ka baka litholoana tse ntle kapa tsa bohata.
Sephetho sa tlhatlhobo e se feela sa bongaka, ha ea lokela ho nahanisisa ka matšoao a bongaka, karabelo ea bongaka, pepeneneng le tlhaiso-leseling e ngoe .
.This reagent e sebelisoa feela bakeng sa serum le liteko tsa plasma. E kanna ea se ke ea fumana litholoana tse nepahetseng ha e sebelisoa bakeng sa li-sampole tse ling tse kang mathe le moroto le jj.

Litšobotsi tsa Ts'ebetso

RLikopano
1.HANDEN JH, ET Al.hama
2.Levinson SS.She li-antibodies tsa heterophphilic le karolo e teng ho itšireletsang ea Immunogay, 199,15: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-14.

Senotlolo sa matšoao a sebelisitsoeng:

	Lisebelisoa tsa bongaka tsa vitro tsa vitro
	Moetsi
	Lebenkele ka 2-30 ℃
	Letsatsi la pheletso
	Se ke oa e sebelisa hape
	Tlhokomeliso
	Kopanya litaelo tsa ho sebelisa

Xiamen Wiz Biotech CO., LTD
Aterese: 3-4 Mobu, The.16 mohaho, Lithuto tsa bongaka tsa Bio-201026, Xiamen, China
Tel: + 86-592-6808278
FekE: + 86-592-6808279