Kitty ea Tloaelo ea Progesterone(fluorescence cluolumochromatographic
Bakeng sa li-vitro tsa ho etsa joalo
Ka kopo bala sephutheloana sena sa sephutheloana pele ho ka sebelisoa le ho latela litaelo. Ho ts'epahala ha liphetho tse tsoelang pele ho ke ke ha netefatsoa haeba ho na le maemo a tsoang ho litaelo tse kenyang sephutheloana sena.

Ts'ebeliso e reriloeng
Kittyston Kitty bakeng sa progesterone (fluoresconcection inshorense ea ho abela mafu . Teko ena e etselitsoe boits'oaro ba bophelo bo botle feela.

Kakaretso
Progesterone ke lihormone tsa bohlokoa tse phethang karolo ea bohlokoa ea ho ea matsatsing 'me e bohlokoa ho boloka bokhachane. Matšoenyeho a Khohlano le Termsterone serum e eketsehile ka potlako kamora ho otsoa. Ke letšoao le tšepahalang la ovulation ea tlhaho kapa ea ho se tsebe.

Molao-motheo oa Ts'ebetso
Membrane ea sesebelisoa sa tlhahlobo e koahetsoe ke conjugate ea BSA le Prog sebakeng sa liteko le poli antiboth sebakeng sa taolo. Marker Pad a koaheloa ke Andre Anti Prog Antiody le Rabbit tg esale pele. Ha ho hlahloba sampole, Prog ea mohlala e kopana le fluorescence e bonoang e anti Prog antiody, 'me a theha motsoako oa ho itšireletsa. Tlas'a ts'ebetso ea likotlo tsa immunochundographmory, lesupi ea mahala e tla kopanngoa le prog membrane. Khatello ea Prog ea mohlala ea mohlala e ka fumanoa ke fluorescence Cluosassay.

Reagents le lisebelisoa tse fanoeng
Mehloli ea sephutheloana sa 25t:
Karete ea liteko ka boeena e kenelletse ka lesika le lesika la 25t
Sampu ea Doungs 25t
Sephutheloana sa sephutheloana 1

Lisebelisoa tse hlokahalang empa ha li fanoe
Mohlala oa mohlala oa mohlala, timer

Ho bokelloa ha mohlala le ho boloka
1.Ke lekoa ke semela e ka ba serum, Heparin Anticoagulant plasma kapa EDTARagulant plasma.
2.Cutcording ho isa mekhoa e tloaelehileng e bokella mehlala. Serum kapa Plasma samne ea plasma e ka bolokoa e haneloa ho 2-8 ℃ bakeng sa matsatsi a 7-5
Mohlala oa 3.All o qoba ho fafatsa molaetsa-thaw.

Ts'ebetso ea Assay
Ka kopo bala lengolo la sesebelisoa sa sesebelisoa le sephutheloana sa ho kenya tlhahlobo pele ho tlhahlobo.
1.Lay ka thoko litsie le mekgatlo tsohle tsa mekgutshi.
2OPEN The Extienter Exatterzer (Wiz-A101), kenya phasewete ea ak'haonte ho latela mokhoa oa ts'ebetso ea sesebelisoa, 'me u kenye sebopeho sa ho lemoha sesebelisoa.
3.scan khoutu ea meno ho netefatsa ntho ea tlhahlobo.
4.Take ka karete ea liteko e tsoang ka mokotleng oa foil.
5. Kopa karete ea liteko ka karete ea karete, hlahloba khoutu ea QR, 'me u khetholle ntho ea tlhahlobo.
6.Sa 20__l serum kapa sampole ea plasma ho sampole e tlamehang ho etsa mohlala, 'me kopanya hantle ..
7.DD 80μl Vple S samle Soluple Tharollo ea mohlala hantle ea karete.
8.Click konopo ea "Standra Standard", kamora metsotso e 10, sesebelisoa se tla bala karete ea tlhahlobo ea sesebelisoa, mme e rekota sephetho sa tlhahlobo.
9.Refer to taeo ea senokoane sa bahlahlobi ba kenngoeng (Wiz-a101).

Liphetho tsa liteko le toloko

.O data e kaholimo ho sephetho sa tlhahlobo ea prog ea prog, 'me ho khothalletsoa hore bolokolohi bo bong le bo bong bo lokela ho theha palo ea baahi ba sebakeng sena. Liphetho tse kaholimo ke tsa ho bua ka eona feela.
.Thotso ea mokhoa ona e sebetsa feela ho li-ranges tse boletsoeng ka mokhoa ona, 'me ha ho na lipelaelo tse tobileng le mekhoa e meng.
.

Ho boloka le ho tsitsa
1.The Kit ke sehatsetsi sa likhoeli tse 18 ho tloha ka letsatsi la tlhahiso. Boloka li-kitsi tse sa sebelisoeng ho 2-30 ° C. Se ke oa bata. Se ke oa sebelisa ntle le letsatsi la ho felloa ke nako.
2.Do ha e bule lape e tiisitsoeng ho fihlela u se u loketse ho etsa tlhahlobo, 'me tlhahlobo e le' ngoe e lokela ho sebelisoa tlasa tikoloho e hlokahalang (Mocheso 2-90%) kahare ho 60 metsotso ka moo ho ka khonehang.
Boitlamo ba 3Samp bo sebelisoa hang-hang kamora ho buloa.

Litemoso le Litlhokomelo
.Ke ka ho ts'oaroa le ho sireletsoa mongobo.
Lipapatso tse ntle tse ntle li tla netefatsoa ke mekhoa e meng.
Lipapatso tsa .Le.
.Do e se e sebelisetsoa ho hlakisoa.
.Do e sa fetohe li-regels tsa li-kits tse nang le lotho e fapaneng.
.Do ho se sebelise likarete tsa liteko le lisebelisoa life kapa life tse lahlehileng.
.MoSisoperation, sampole e feteletseng kapa e nyane e ka lebisa ho kheloha.

LHo Etsisoa
.A ka assays e 'ngoe le e' ngoe ea li-antibodies, monyetla o teng ho li-antibodies tsa anti-mouse (Hama) ka mokhoabong. Mehlala ea bakuli ba fumaneng litokisetso tsa li-antibodies tsa menoclon bakeng sa tlhahlobo kapa kalafo e ka ba le hamma. Mehlala e joalo e ka baka litholoana tse ntle kapa tsa bohata.
Sephetho sa tlhatlhobo e se feela sa bongaka, ha ea lokela ho nahanisisa ka matšoao a bongaka, karabelo ea bongaka, pepeneneng le tlhaiso-leseling e ngoe .
.This reagent e sebelisoa feela bakeng sa serum le liteko tsa plasma. E kanna ea se ke ea fumana litholoana tse nepahetseng ha e sebelisoa bakeng sa li-sampole tse ling tse kang mathe le moroto le jj.

Litšobotsi tsa Ts'ebetso

RLikopano
1.HANDEN JH, ET Al.hama
2.Levinson SS.She li-antibodies tsa heterophphilic le karolo e teng ho itšireletsang ea Immunogay, 199,15: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-14.

Senotlolo sa matšoao a sebelisitsoeng:

	Lisebelisoa tsa bongaka tsa vitro tsa vitro
	Moetsi
	Lebenkele ka 2-30 ℃
	Letsatsi la pheletso
	Se ke oa e sebelisa hape
	Tlhokomeliso
	Kopanya litaelo tsa ho sebelisa

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