Kitty ea Diagnotic bakeng sa Femal Matlapa(Fluorescence cluolumochromasographic
Bakeng sa li-vitro tsa ho etsa joalo
Ka kopo bala sephutheloana sena sa sephutheloana pele ho ka sebelisoa le ho latela litaelo. Ho ts'epahala ha liphetho tse tsoelang pele ho ke ke ha netefatsoa haeba ho na le maemo a tsoang ho litaelo tse kenyang sephutheloana sena.

Ts'ebeliso e reriloeng
Tlhatlhobo ea tlhahlobo ea bo-'mōmo tsa lefutso ea masapo (bofokoli bo phophomang Teko ena e etselitsoe boits'oaro ba bophelo bo botle feela.

Kakaretso
Ho akheha hanyane ka hanyane ho etsa hore fobe e be le bohlokoa ba fob, e leng mokhoa oa bohlokoa a tsoang maling, e fumaneha bakeng sa lefu la likopi.

Molao-motheo oa Ts'ebetso
Strip e anti-fob e koahelang antibody sebakeng sa liteko, tse khabisitsoeng ke li-choromatograph tsa "choromatography Pad pad e koahetsoe ke fereorescence e bitsoang anti-fob anti-fob esale pele. Ha u ntse u batla sampole e nepahetseng, fobe ea mohlala e ka tsoakoa le phofo e ngotsoeng le fluorescence e bitsoang anti-fob motsoako oa ho itšireletsa. Joalokaha motsoako o lumelloa ho fallela moqapi oa teko, fob e thata e hapiloe ke anti-fob e koahelang antibody on a anti-form. Bokaholimo bo ferekane bo hlophisitsoe hantle le litaba tsa fob. Fob ea mohlala e ka fumanoa ke senokoane sa boikhathollo sa boikhathollo.

Reagents le lisebelisoa tse fanoeng
Mehloli ea sephutheloana sa 25t:
Karete ea liteko ka boeena e kenelletse ka lesika le lesika la 25t
Sampu ea Doungs 25t
Sephutheloana sa sephutheloana 1
Lisebelisoa tse hlokahalang empa ha li fanoe
Mohlala oa mohlala oa mohlala, timer

Ho bokelloa ha mohlala le ho boloka
1. Fetola setshelo se hloekileng sa ho bokella sampole ea counges, mme e lekoa hanghang. Haeba e ke ke ea hlahlojoa hang-hang, ka kopo e bolokiloe ho 2-8 ° C ka matsatsi a 3 kapa ka tlase ho likhoeli tse 6.
2. Ka 'mele oa pompong, kenngoeng ka sampole ea faeleng, pheta ketso e fapaneng le ho sisinya, kapa ho sisinya lere ea sa sisinyeheng Makhona a batho ba ka bang 50mg Founse, 'me a bea thabeng ea sampole ea fasene e nang le sampole ea sampole,' me u kotula ka thata.
3. Khanya ea pepeli e senyehileng ea Pipete e lahliloeng e nka mohlala oa faces ho mokuli oa lets'ollo, ebe o eketsa marotholi a 3 le ho sisinyeha.
Lintlha:
1.ame e se nang thuso-thaw.
2.Hasa sampole ea mocheso oa kamore pele o sebelisoa.
Ts'ebetso ea Assay
Ka kopo bala lengolo la sesebelisoa sa sesebelisoa le sephutheloana sa ho kenya tlhahlobo pele ho tlhahlobo.
1.Lay ka thoko litsie le mekgatlo tsohle tsa mekgutshi.
2.O Sebui sa 'mele se keteketsi (Wiz-A101), kenya phasewete ea ak'haonte ho latela mokhoa oa ts'ebetso ea sesebelisoa,' me u kenye sebopeho sa ho lemoha sesebelisoa.
3.scan khoutu ea meno ho netefatsa ntho ea tlhahlobo.
4.Take ka karete ea liteko e tsoang ka mokotleng oa foil.
5. Kopa karete ea liteko ka karete ea karete, hlahloba khoutu ea QR, 'me u khetholle ntho ea tlhahlobo.
6.Remove the cap ho tsoa ho sampole thabeng ea pele ea drop ea pele, eketsa 100ul) ha ho na li-sompole tsa mohlala
7.Click konopo ea "Teko ea Teko", kamora metsotso e 15, sesebelisoa se tla bala karete ea liteko, mme e rekota sephetho sa tlhahlobo.
8.Refer pakelong ea sebui sa 'mele se ke keng sa hlahlojoa (Wiz-A101).
Melao e lebelletsoeng
Fob <0,2μg / ml
Ho khothalletsoa hore theborato e 'ngoe le e' ngoe e tiisa hore e tloaelehile e emela baahi ba eona ba bakuli.
Liphetho tsa liteko le toloko
1.Ke fob ho sampole ke hore 0,2μg / ml, mme e lokela ho hlahisa phetoho ea boemo ba mmele. Ka sebele litholoana ha li na tšabo 'me ka sebele li lokela ho fumanoa e na le matšoao a ho tsoala.
2.Ba sephetho sa mokhoa ona ho sebetsa feela ho li-Wanges tse thehiloeng ka mokhoa ona, 'me ha ho na lipelaelo tse tobileng le mekhoa e meng.
3.Other factors can also cause errors in detection results, including technical reasons, operational errors and other sample factors.

Ho boloka le ho tsitsa
1.The Kit ke sehatsetsi sa likhoeli tse 18 ho tloha ka letsatsi la tlhahiso. Boloka li-kitsi tse sa sebelisoeng ho 2-30 ° C. Se ke oa bata. Se ke oa sebelisa ntle le letsatsi la ho felloa ke nako.
2.Do ha e bule lape e tiisitsoeng ho fihlela u se u loketse ho etsa tlhahlobo, 'me tlhahlobo e le' ngoe e lokela ho sebelisoa tlasa tikoloho e hlokahalang (Mocheso 2-90%) kahare ho 60 metsotso ka moo ho ka khonehang.
3.Lample e lenngoeng e sebelisoa hang-hang kamora ho buloa.

Litemoso le Litlhokomelo
.Ke ka ho ts'oaroa le ho sireletsoa mongobo.
Lipapatso tse ntle tse ntle li tla netefatsoa ke mekhoa e meng.
Lipapatso tsa .Le.
.Do e se e sebelisetsoa ho hlakisoa.
.Do e sa fetohe li-regels tsa li-kits tse nang le lotho e fapaneng.
.Do ho se sebelise likarete tsa liteko le lisebelisoa life kapa life tse lahlehileng.
.MoSisoperation, sampole e feteletseng kapa e nyane e ka lebisa ho kheloha.

LHo Etsisoa
.A ka assays e 'ngoe le e' ngoe ea li-antibodies, monyetla o teng ho li-antibodies tsa anti-mouse (Hama) ka mokhoabong. Mehlala ea bakuli ba fumaneng litokisetso tsa li-antibodies tsa menoclon bakeng sa tlhahlobo kapa kalafo e ka ba le hamma. Mehlala e joalo e ka baka litholoana tse ntle kapa tsa bohata.
Sephetho sa tlhatlhobo e se feela sa bongaka, ha ea lokela ho nahanisisa ka matšoao a bongaka, karabelo ea bongaka, pepeneneng le tlhaiso-leseling e ngoe .
.This reagent e sebelisoa feela bakeng sa liteko tsa fecal. E kanna ea se ke ea fumana litholoana tse nepahetseng ha e sebelisoa bakeng sa li-sampole tse ling tse kang mathe le moroto le jj.

Litšobotsi tsa Ts'ebetso

RLikopano
1.HANDEN JH, ET Al.hama
2.Levinson SS.She li-antibodies tsa heterophphilic le karolo e teng ho itšireletsang ea Immunogay, 199,15: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-14.

 Senotlolo sa matšoao a sebelisitsoeng:

	Lisebelisoa tsa bongaka tsa vitro tsa vitro
	Moetsi
	Lebenkele ka 2-30 ℃
	Letsatsi la pheletso
	Se ke oa e sebelisa hape
	Tlhokomeliso
	Kopanya litaelo tsa ho sebelisa

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