Diagnostic Kit bakeng saCalprotectin(Tlhahlobo ea Fluorescence Immunochromatographic)
Bakeng sa tšebeliso ea in vitro diagnostic feela
Ka kopo bala sephutheloana sena ka hloko pele u se sebelisa 'me u latele litaelo ka hloko. Ho tšepahala ha liphetho tsa tlhahlobo ho ke ke ha netefatsoa haeba ho na le liphapang life kapa life tse tsoang ho litaelo tse kenyelletsong ena ea sephutheloana.

TŠEBELETSO TŠEBELETSO
Diagnostic Kit bakeng saCalprotectin(Cal) e loketse ho etsa qeto ea palo ea mantle a batho Cal ka fluorescence immunochromatographic assay, e nang le bohlokoa ba ho hlahloba mafu a ho ruruha. Teko ena e reretsoe ho sebelisoa ke litsebi tsa bophelo bo botle feela.

KAKARETSO
Cal ke heterodimer, e entsoeng ka MRP 8 le MRP 14^[1]. E fumaneha ka har'a cytoplasm ea neutrophils 'me e hlahisoa ka lisele tsa mononuclear. Cal ke protheine ea acute phase, e na le mohato o tsitsitseng hantle hoo e ka bang beke e le 'ngoe mantleng a motho, e ikemiselitse ho ba letšoao la lefu la ho ruruha ha mala.^[2-3]. Thepa ke teko e bonolo, e bonoang ea palo e lemohang Cal mantleng a motho, e na le kutlo e phahameng ea ho lemoha le ho hlaka ho matla. Teko e ipapisitse le maemo a holimo a li-antibodies tse peli tsa sandwich le litekheniki tsa tlhahlobo ea tlhahlobo ea fluorescence immunochromatographic, tse ka fanang ka sephetho nakong ea metsotso e 15.

MOLAO OA MOKHOA
Letlapa le na le anti-Cal coating antibody sebakeng sa liteko, se tlamelletsoeng ho membrane chromatography esale pele. Lable pad e koahetsoe ke fluorescence e ngotsoeng anti-Cal antibody esale pele. Ha u etsa liteko tsa sampole e ntle, Cal ka sampoleng e ka tsoakoa le fluorescence e ngotsoeng anti-Cal antibody, 'me ea etsa motsoako oa ho itšireletsa mafung. Ha motsoako o lumelloa ho falla haufi le mohala oa teko, Cal conjugate complex e hapuoa ke anti-Cal coating antibody ka lera 'me e etsa e rarahaneng. Matla a fluorescence a amana hantle le litaba tsa Cal. Cal in sampole e ka bonoa ka fluorescence immunoassay analyzer.

LI-REAGENTS LE LITŠOANTŠISO TSE FEPANG

25T likarolo tsa sephutheloana:
Karete ea liteko ka bonngoe e kenngoe ka mokotla oa desiccant 25T
Mehlala ea li-diluents 25T
Kenya sephutheloana 1

LITLHAKISO TSE HLOKAHANG EMPA HA LI ABONE
Sets'oants'o sa pokello ea mohlala, timer

POKELLO MOHLALA LE POLOKO
1.Sebelisa setshelo se hloekileng se lahloang ho bokella sampole ea mantle a macha, 'me u hlahlojoe hang hang. Haeba e ke ke ea hlahlojoa hang-hang, ka kopo e bolokoe ho 2-8 ° C matsatsi a 3 kapa ka tlase ho -15 ° C bakeng sa likhoeli tse 6.
2. Ntša thupa ea sampole, e kenngoe ka har'a sampole ea mantle, pheta ketso ka makhetlo a 3, nka likarolo tse fapaneng tsa sampole ea mantle nako le nako, ebe u khutlisetsa thupa ea sampole, korola ka thata 'me u sisinye hantle, Kapa sebelisa thupa ea sampole e nkiloeng. sampuli ea mantle e ka bang 50mg, 'me u kenye ka har'a sampole ea mantle e nang le sampole ea dilution, ebe u e thiba ka thata.
3. Sebelisa sampole ea pipette e lahloang, nka sampole ea mantle ho mokuli ea tšoeroeng ke lets'ollo, ebe u eketsa marotholi a 3 (hoo e ka bang 100µL) ka har'a tube ea sampole ea mantle 'me u sisinyehe hantle.

Lintlha:
1. Qoba lipotoloho tsa ho qhibiliha ha leqhoa.
2.Thaw disampole mocheso oa kamore pele o sebelisoa.

TS'ELISO MOKHOA

Ka kopo bala bukana ea ts'ebetso ea lisebelisoa le sephutheloana pele u etsa liteko.
1.Beha ka thōko li-reagents tsohle le mehlala ho mocheso oa kamore.
2.Open Portable Immune Analyzer (WIZ-A101), kenya phasewete ea ho kena ka akhaonto ho latela mokhoa oa ho sebetsa oa sesebelisoa, 'me u kenye sebopeho sa ho lemoha.
3.Skena khoutu ea boitsebahatso ho netefatsa ntho ea teko.
4.Ntša karete ea teko ka mokotleng oa foil.
5.Kenya karete ea teko ka har'a sekotjana sa karete, hlahloba khoutu ea QR, 'me u tsebe hore na teko ke efe.
6.Tlosa sekoahelo ho tswa ho tube ea mohlala 'me u lahle marotholi a mabeli a pele a hlapollotsoeng, eketsa marotholi a 3 (hoo e ka bang 100uL) ha ho bubble e hlapollotsoeng ka sampuli e otlolohileng' me butle-butle e be sampole selibeng sa karete e nang le dispette e fanoeng.
7.Tobetsa konopo ea "teko e tloaelehileng", ka mor'a metsotso ea 15, sesebelisoa se tla lemoha karete ea teko, se ka bala liphetho ho tloha skrineng sa pontšo ea sesebelisoa, 'me se tlalehe / se hatise liphetho tsa liteko.
8.Sheba taelo ea Portable Immune Analyzer(WIZ-A101).

TEBELLO MATHEO
Khalo e ka tlase ho 60μg/g
Ho khothaletsoa hore laboratori ka 'ngoe e thehe mofuta oa eona o tloaelehileng o emelang palo ea bakuli ba eona.

LIPOLELO TSA TEKO
1. Cal ka sampuli e feta 60μg/g, 'me e lokela ho thibela phetoho ea boemo ba fisioloji. Liphetho li hlile li sa tloaeleha 'me li lokela ho fumanoa li e-na le matšoao a kliniki.
2.Liphello tsa mokhoa ona li sebetsa feela ho lihlopha tsa litšupiso tse thehiloeng ka mokhoa ona, 'me ha ho na ho bapisoa ka ho toba le mekhoa e meng.
3.Mabaka a mang a ka boela a baka liphoso liphethong tsa ho lemoha, ho kenyelletsa mabaka a tekheniki, liphoso tsa ts'ebetso le lintlha tse ling tsa mohlala.

POLOKO LE TSAMAISO
1. Thepa ke likhoeli tse 18 ho tloha ka letsatsi la tlhahiso. Boloka lisebelisoa tse sa sebelisoeng mocheso oa 2-30 ° C. U SE KE UA QETELA. Se ke oa sebelisa ho feta letsatsi la ho felloa ke nako.
2. U se ke ua bula mokotla o tiisitsoeng ho fihlela u se u itokiselitse ho etsa tlhahlobo, 'me tlhahlobo ea tšebeliso e le' ngoe e khothaletsoa hore e sebelisoe tlas'a tikoloho e hlokahalang (mocheso 2-35 ℃, mongobo 40-90%) nakong ea metsotso e 60 kapele. ka moo ho ka kgonehang.
3.Sample diluent e sebelisoa hang ka mor'a ho buloa.

TLHOKOMELISO LE TS'ELISO
.Kit e lokela ho koaloa le ho sireletsoa khahlanong le mongobo.
.Mehlala eohle e nepahetseng e tla netefatsoa ke mekhoa e meng.
.Mehlala eohle e tla ts'oaroa joalo ka ts'ilafalo e ka bang teng.
. U SE KE UA sebelisa reagent e felloang ke nako.
.O SE KE oa fapanyetsana li-reagents har'a lisebelisoa tse nang le lotho e fapaneng No.
.O SE KE oa boela oa sebelisa likarete tsa tlhahlobo le lisebelisoa life kapa life tse ka lahloang.
.Ho se sebetse hantle, sampole e ngata kapa e nyane e ka lebisa ho kheloha ha sephetho.

MOEKETSO
.Joalo ka tlhahlobo efe kapa efe e sebelisang li-antibodies tsa litoeba, monyetla o teng oa ho kena-kenana le li-anti-toeba tsa batho (HAMA) setšoantšong. Mehlala e tsoang ho bakuli ba fumaneng litokisetso tsa li-antibodies tsa monoclonal bakeng sa ho hlahlojoa kapa ho phekoloa li ka ba le HAMA. Mehlala e joalo e ka baka litlamorao tse mpe kapa tse fosahetseng.
.Sephetho sena sa tlhahlobo ke feela bakeng sa referense ea kliniki, ha ea lokela ho sebetsa e le eona feela motheo oa tlhahlobo ea bongaka le kalafo, taolo ea bakuli e lokela ho nahanoa ka botlalo hammoho le matšoao a eona, nalane ea bongaka, tlhahlobo e 'ngoe ea laboratori, karabo ea kalafo, lefu la seoa le lintlha tse ling. .
.Sehlahisoa sena se sebelisoa feela litekong tsa mantle. E kanna ea se fumane sephetho se nepahetseng ha se sebelisetsoa lisampole tse ling joalo ka mathe le moroto joalo-joalo.

LIEKETSENG TS'EBETSO

LIEKETSENG
1.Li,G.&Y.L.Li.Kamano pakeng tsa calcium le lefu la kliniki[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al.The Clinical Study of calamine le lactoferrin ho bakuli ba nang le ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al.Phuputso e mabapi le kamano pakeng tsa fecal calamine le lefu la ho ruruha ha mala[J]. Theknoloji ea Saense le Boenjiniere,2010-03,10(8)

Senotlolo sa matšoao a sebelisitsoeng:

	Sesebelisoa sa Bongaka sa In Vitro Diagnostic Medical
	Moetsi
	Boloka ho 2-30 ℃
	Letsatsi la pheletso
	U se ke Ua Sebelisa Hape
	TLHOKOMELO
	Sheba Litaelo Bakeng sa Tšebeliso

Xiamen Wiz Biotech CO., LTD
Aterese:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Mohala: + 86-592-6808278
Fax: + 86-592-6808279