Kitty ea Tloaelo ea Vitamity D(fluorescence cluolumochromatographic
Bakeng sa li-vitro tsa ho etsa joalo
Ka kopo bala sephutheloana sena sa sephutheloana pele ho ka sebelisoa le ho latela litaelo. Ho ts'epahala ha liphetho tse tsoelang pele ho ke ke ha netefatsoa haeba ho na le maemo a tsoang ho litaelo tse kenyang sephutheloana sena.

Ts'ebeliso e reriloeng
Kittynostic Kit bakeng sa vithamine D (e ferekane la ho hlasela vithamine D (Oh) VD) Samle e tlameha ho netefatsoa ke mekhoa e meng. Teko ena e etselitsoe boits'oaro ba bophelo bo botle feela.

Kakaretso
Vitamin D ke vithamini 'me le eona ermone ea steroid, haholo-holo ho kenyelletsa vd2 le Vd3, eo letsoho la hae le tšoanang haholo. Vitamin D3 Le D2 li fetoloa Vitaminl Vitaminl Vitamin D (Ho kenyelletsa Vitamin D3 le D2). 25- (Oh) VD ka 'mele oa motho, ho tsitsipana ho tsitsipanang. 25- (Oh) VD e bonts'a boholo ba vithamine D, le bokhoni ba ho fetoloa ba ho hlahloba boemo ba vithamine D.the bo ka fana ka sephetho sa ho itšireletsa ho itširelembocTography

Molao-motheo oa Ts'ebetso
Membrane ea sesebelisoa sa tlhahlobo e koahetsoe ke conjugate ea BSA le 25- (Oh) VD ho Setereke sa Teko le Pōli Anti Rabbit sebakeng sa taolo. Marker Pad li kopantsoe le letšoao la fluorescceccence Anti 25- (Oh) Vd Antibody le Rabbit trig esale pele. Ha ho hlahloba sampole, 25- (Oh) VD ho sampole e le 'ngoe e kopaneng e nang le fluorescence e tšoailoeng anti 25- (Oh) Vd motsoako oa ho itšireletsa. Tlas'a ketso ea likotlo tsa immunochundogramme tse fetisitsoeng ke sehlekehleke sa ho sesa

Reagents le lisebelisoa tse fanoeng

Mehloli ea sephutheloana sa 25t:
Card. Tilly ka foil e entsoe ka lesika la botsitso 25t
.A tharollo 25T
.B tharollo 1
.PaChelage e kenya 1

Lisebelisoa tse hlokahalang empa ha li fanoe
Mohlala oa mohlala oa mohlala, timer

Ho bokelloa ha mohlala le ho boloka
1.Ke lekoa ke semela e ka ba serum, Heparin Anticoagulant plasma kapa EDTARagulant plasma.
2.Cutcording ho isa mekhoa e tloaelehileng e bokella mehlala. Serum kapa Plasma samne ea plasma e ka bolokoa e haneloa ho 2-8 ℃ bakeng sa matsatsi a 7-5
Mohlala oa 3.All o qoba ho fafatsa molaetsa-thaw.

Ts'ebetso ea Assay
Ts'ebetso ea liteko ea sesebelisoa e bona bukana ea Iltrananglyzer. Ts'ebetso ea liteko e ratoang e ntse e latela
1.Lay ka thoko litsie le mekgatlo tsohle tsa mekgutshi.
2.O Sebui sa 'mele se keteketsi (Wiz-A101), kenya phasewete ea ak'haonte ho latela mokhoa oa ts'ebetso ea sesebelisoa,' me u kenye sebopeho sa ho lemoha sesebelisoa.
3.scan khoutu ea meno ho netefatsa ntho ea tlhahlobo.
4.Take ka karete ea liteko e tsoang ka mokotleng oa foil.
5. Kopa karete ea liteko ka karete ea karete, hlahloba khoutu ea QR, 'me u khetholle ntho ea tlhahlobo.
6.ADD 30__L sampole kapa serusma sampole ka tharollo, 'me u kopanya hantle.
7.DADDEkholo ea 50μl BOR ka motsoako o ka pele, 'me o kopanya hantle.
8.Tlohela motsoako bakeng sa metsotso e 15.
9. Beled 80μl motsoako ho ea mohlala hantle oa karete.
10.Click konopo ea "Teko ea Teko", kamora metsotso e 10, sesebelisoa se tla bala karete ea tlhahlobo ea sesebelisoa, mme e rekota liphetho tsa tlhahlobo.
11.REFT to taeong ea sebui sa 'mele se ke keng sa hlahlojoa (Wiz-a101).

Melao e lebelletsoeng
25- (Oh) VD e tloaelehileng: 30-100ng / ml
Ho khothalletsoa hore theborato e 'ngoe le e' ngoe e tiisa hore e tloaelehile e emela baahi ba eona ba bakuli.

Liphetho tsa liteko le toloko
.Tota data e kaholimo ho nako ea ho khetholloa bakeng sa data ena, 'me ho khothaletsoa hore mosebetsi o mong le o mong o ka theha nako ea palo ea bana sebakeng sena.
.Ka likhohlano tsa 25- (Oh) VD e phahame ho feta moo, 'me karabelo ea kelello e lokela ho khethoa, e lokela ho kopanya motho ea sa tloaelehang.
.Thotso ea mokhoa ona e sebetsa feela ho ratoang ho ratoang ke mokhoa ona, 'me liphetho ha li tšoane ka kotloloho le mekhoa e meng ka kotloloho.
.

Ho boloka le ho tsitsa
.Ke kit ke sehatsetsi sa likhoeli tse 18 ho tloha ka letsatsi la tlhahiso. Boloka li-kitsi tse sa sebelisoeng ho 2-30 ° C. Se ke oa bata. Se ke oa sebelisa ntle le letsatsi la ho felloa ke nako.
.Do ho se bule lapera e tiisitsoeng ho fihlela u se u loketse ho etsa tlhahlobo, 'me tlhahlobo e le' ngoe e lokela ho sebelisoa tlasa tikoloho ea 40 ka potlako kamoo ho ka khonehang.
.Sumple e kopane hangata kamora ho buloa.

Litemoso le Litlhokomelo
.Ke ka ho ts'oaroa le ho sireletsoa mongobo.
Lipapatso tse ntle tse ntle li tla netefatsoa ke mekhoa e meng.
Lipapatso tsa .Le.
.Do e se e sebelisetsoa ho hlakisoa.
.Do e sa fetohe li-regels tsa li-kits tse nang le lotho e fapaneng.
.Do ho se sebelise likarete tsa liteko le lisebelisoa life kapa life tse lahlehileng.
.MoSisoperation, sampole e feteletseng kapa e nyane e ka lebisa ho kheloha.

LHo Etsisoa
.A ka assays e 'ngoe le e' ngoe ea li-antibodies, monyetla o teng ho li-antibodies tsa anti-mouse (Hama) ka mokhoabong. Mehlala ea bakuli ba fumaneng litokisetso tsa li-antibodies tsa menoclon bakeng sa tlhahlobo kapa kalafo e ka ba le hamma. Mehlala e joalo e ka baka litholoana tse ntle kapa tsa bohata.
Sephetho sa tlhatlhobo ea tlhahlobo ke feela bakeng sa tlhahlobo ea bongaka, bakuli ba lokela ho nahanisisa ka matšoao a bongaka, karabelo ea bongaka, pepere e ling.
.This reagent e sebelisoa feela bakeng sa serum le liteko tsa plasma. E kanna ea se ke ea fumana litholoana tse nepahetseng ha e sebelisoa bakeng sa li-sampole tse ling tse kang mathe le moroto le jj.

Litšobotsi tsa Ts'ebetso

RLikopano

1.HANDEN JH, ET Al.hama
2.Levinson SS.She li-antibodies tsa heterophphilic le karolo e teng ho itšireletsang ea Immunogay, 199,15: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-114: 108-14.

Senotlolo sa matšoao a sebelisitsoeng:

	Lisebelisoa tsa bongaka tsa vitro tsa vitro
	Moetsi
	Lebenkele ka 2-30 ℃
	Letsatsi la pheletso
	Se ke oa e sebelisa hape
	Tlhokomeliso
	Kopanya litaelo tsa ho sebelisa

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