Xirmada ogaanshaha ee Hormoonka Kicinta ee Thyroid-ka (qiimaynta immunochromatographic fluorescence)
Qalabka ogaanshaha eeHormoonka Kicinta Thyroid-kabaaritaanka immunochromatographic)
Isticmaalka ogaanshaha in vitro ee kaliya
Fadlan si taxadar leh u akhri xidhmadan xidhmadan ka hor intaanad isticmaalin oo si adag u raac tilmaamaha. Kalsoonida natiijooyinka qiimaynta lama dammaanad qaadi karo haddii ay jiraan wax ka leexasho ah tilmaamaha ku jira xidhmadan.
ISTICMAALKA KULAN
Qalabka ogaanshaha ee Hormoonka Kicinta ee Thyroid (Fluorescence immunochromatographic assay) waa baarista fluorescence immunochromatographic asay ee lagu ogaanayo tirada Hormoonka Kicinta Thyroid (TSH) ee serum ama balasmaha, kaas oo inta badan loo isticmaalo qiimeynta shaqada pituitary-thyroid. Dhammaan muunada wanaagsan waa in lagu xaqiijiyo habab kale. Baaritaankan waxaa loogu talagalay in loo isticmaalo xirfadlayaasha daryeelka caafimaadka oo keliya.
KOOBAN
Hawlaha ugu muhiimsan ee TSH: 1, kor u sii daynta hoormoonka tayroodh, 2, kor u falgalka T4, T3, oo ay ku jiraan xoojinta shaqada bamka iodine, kor u qaadida waxqabadka peroxidase, kor u synthesis of thyroid globulin iyo tyrosine iodide.
MABADA HABKA
Xuubabka aaladda waxa lagu dahaadhay antibody TSH ee gobolka tijaabada iyo ariga ka hortagga bakaylaha IgG ee gobolka kantaroolka. suufka suufka ah waxaa dahaarka leh fluorescence oo ku calaamadsan antibody TSH iyo bakayle IgG ka hor. Marka la tijaabiyo muunada togan, antigen-ka TSH ee muunada ku jira waxa ay ku daraan fluorescence lagu calaamadiyay antibody-ka TSH, oo waxay sameeyaan isku-dar difaac. Under ficilka immunochromatography ah, socodka kakan ee jihada warqad nuugista, marka adag maray gobolka imtixaanka, waxaa la isku daray antibody daahan anti TSH, foomamka adag cusub. heerka TSH si togan la xidhiidha signal fluorescence, iyo fiirsashada ee TSH. muunad ahaan waxa lagu ogaan karaa iyada oo la adeegsanayo fluorescence immunoassay.
AALADAHA IYO AALADAHA LA BIXIYAY
25T qaybaha xirmo
.Kaarka imtixaanku si gooni gooni ah bireed ugu xidhan yahay 25T
.Sample diluents
.Baakadaha gelin
AALADAHA LOO BAAHAN YAHAY LAAKIIN AAN LA BIXIN
Tusaalaha weelka aruurinta, saacada
Tusaalaha Uruurinta iyo Kaydinta
1.Samballada la tijaabiyay waxay noqon karaan serum, heparin anticoagulant plasma ama EDTA plasma anticoagulant plasma.
2.Sida laga soo xigtay farsamooyinka caadiga ah ururiyaan muunad. Saambalka serum ama balaasmaha waxa lagu hayn karaa qaboojiyaha 2-8 ℃ muddo 7 maalmood ah iyo ilaalinta qaylada oo ka hooseeya -15°C muddo 6 bilood ah.
3.Dhammaan muunada iska ilaali wareegyada barafowga.
HABKA ASSAY
Habka tijaabada ee qalabka eeg buug-yaraha difaaca jirka. Habka tijaabada reagent waa sida soo socota
1. Geli dhammaan reagen-yaasha iyo shaybaarada ilaa heerkulka qolka.
2.Fur la qaadi karo Immune Analyzer (WIZ-A101), geli lambarka sirta ah ee xisaabta sida habka hawlgalka ee qalabka, oo geli interface ogaanshaha.
3.Scan code aqoonsiga si loo xaqiijiyo shayga tijaabada.
4.Ka soo saar kaadhka tijaabada bacda qashinka.
5. Geli kaadhka tijaabada booska kaadhka, iskaan koodhka QR, oo go'aami shayga tijaabada.
6.Ku dar 20μL serum ama muunad balasma si muunad dareere ah, oo si fiican isku qas.
7.Ku dar xal muunad 80μL si aad muunad fiican u hesho kaadhka.
8. Guji badhanka "tijaabada caadiga ah", 15 daqiiqo ka dib, qalabku wuxuu si toos ah u ogaan doonaa kaarka imtixaanka, wuxuu akhrin karaa natiijooyinka shaashadda qalabka, oo duubo / daabac natiijooyinka imtixaanka.
9.Tixraac tilmaamaha Falanqeeyaha difaaca jirka ee la qaadi karo (WIZ-A101).
Natiijooyinka Imtixaanka iyo Fasiraadda
Xogta kor ku xusan waa natiijada baaritaanka reagent TSH, waxaana la soo jeediyay in shaybaar kastaa sameeyo qiimeyaal kala duwan oo lagu ogaanayo TSH oo ku habboon dadweynaha gobolkan. Natiijooyinka kor ku xusan waa tixraac kaliya.
.Natiijooyinka habkani waxa kaliya oo lagu dabaqi karaa qiyaasaha tixraaca ee lagu sameeyay habkan, mana jirto wax si toos ah u barbar dhigaya hababka kale.
Qodobada kale waxay sidoo kale keeni karaan khaladaad natiijooyinka ogaanshaha, oo ay ku jiraan sababo farsamo, khaladaad hawleed iyo arrimo kale oo muunad ah.
Kaydinta iyo Xasiloonida
1.Qalabka waa 18 bilood oo cimri dherer ah laga bilaabo taariikhda la soo saaray. Ku kaydi xirmooyinka aan la isticmaalin 2-30°C. HA BAFAYN. Ha isticmaalin wixii ka dambeeya taariikhda dhicitaanka.
2.Ha furin boorsada xidhan ilaa aad diyaar u tahay inaad samayso imtixaan, waxaana la soo jeediyay tijaabada hal-isticmaalka in lagu isticmaalo deegaanka loo baahan yahay (heerkulka 2-35 ℃, huurka 40-90%) 60 daqiiqo gudahood sida ugu macquulsan.
3.Sample diluent waxaa loo isticmaalaa isla markiiba ka dib marka la furo.
DIGNIIN IYO DIGTOONAADIN
Xirmada waa in la xidhaa lagana ilaaliyaa qoyaanka.
.Dhammaan shaybaarrada togan waa in lagu ansixiyo habab kale.
.Dhammaan shaybaarrada waa in loola dhaqmaa sidii kuwo wasakhaysan.
.HA isticmaalin reagen dhacay.
.Ha isku badalin reajentiyeyaasha xirmooyinka leh tiro kala duwan
Dib ha u isticmaalin kaadhadhka tijaabada iyo agabka la tuuri karo.
Xumaan-xumo, muunad xad-dhaaf ah ama yar waxay keeni kartaa weecsanaan natiijada.
LKU DAYDA
Si la mid ah baaritaan kasta oo isticmaalaya unugyada difaaca jirka ee jiirka, suurtogalnimada ayaa jirta faragelinta unugyada ka hortagga jiirka ee aadanaha (HAMA) ee muunadda. Noocyada bukaannada helay diyaarinta unugyada difaaca jirka ee monoclonal si loo baaro ama daawaynta waxaa ku jiri kara HAMA. Tijaabooyinka noocan oo kale ah waxay keeni karaan natiijooyin togan ama been abuur ah.
.Natiijooyinka baaritaankan ayaa kaliya loogu talagalay tixraaca bukaan-socodka, waa inaysan u adeegin aasaaska kaliya ee ogaanshaha iyo daaweynta bukaan-socodka, maamulka bukaan-socodka bukaan-socodka waa in ay noqdaan kuwo tixgelin ballaaran leh oo ay weheliyaan calaamadaha, taariikhda caafimaadka, baaritaanka shaybaarka kale, jawaab-celinta daaweynta, cudurrada faafa iyo macluumaadka kale. .
Reagent-kan waxa kaliya loo isticmaalaa baadhitaanada serum iyo balasmaha. Waxaa laga yaabaa inaysan helin natiijo sax ah marka loo isticmaalo muunado kale sida candhuufta iyo kaadida iyo iwm.
Tilmaamaha Waxqabadka
Linearity | 0.5μIU/ml ilaa 100μIU/ml | leexashada qaraabo: -15% ilaa +15%. |
Isku xidhka tooska ah:(r)≥0.9900 | ||
Saxnaanta | Heerka soo kabashada waa in ay ahaataa 85% -115%. | |
Ku celcelinta | CV≤15% | |
Gaar ahaan(Mid ka mid ah walxaha ku jira faragelinta la tijaabiyay ma faragelin baaritaanka) | Faragelin | Isku-duubnida dhex-galka ah |
HCG | 2000mIU/ml | |
FSH | 500mIU/ml | |
LH | 500mIU/ml |
TIXRAACYADA
1.Hansen JH, iyo al.HAMA Faragelinta Murine Monoclonal Antibody-based Immunoassays[J].J ee Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Dabeecada Heterophilic Antibodies iyo Doorka Faragelinta Immunoassay[J].J ee Clin Immunoassay,1992,15:108-114.
Furaha calaamadaha la isticmaalay:
In Vitro Diagnostic Device Medical | |
Soo saaraha | |
Ku kaydi at 2-30 ℃ | |
Taariikhda dhicitaanka | |
Dib ha u isticmaalin | |
TAXADIR | |
La tasho Tilmaamaha Isticmaalka |
Hal qayb oo ka mid ah Xiamen Wiz Biotech Co.,LTD
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