Xirmada ogaanshaha ee Hormoonka Luteinizing ( qiimaynta immunochromatographic fluorescence)
Qalabka ogaanshaha eeHormoonka LuteinizingQiimaynta immunochromatographic fluorescence)
Isticmaalka ogaanshaha in vitro ee kaliya
Fadlan si taxadar leh u akhri xidhmadan xidhmadan ka hor intaanad isticmaalin oo si adag u raac tilmaamaha. Kalsoonida natiijooyinka qiimaynta lama dammaanad qaadi karo haddii ay jiraan wax ka leexasho ah tilmaamaha ku jira xidhmadan.
ISTICMAALKA KULAN
Qalabka ogaanshaha ee Hormoonka Luteinizing (Fluorescence immunochromatographic assay) waa tijaabo immunochromatographic fluorescence ah oo loogu talagalay ogaanshaha tirada ee Hormoonka Luteinizing (LH) ee serum ama balaasmaha aadanaha, kaas oo inta badan loo isticmaalo qiimeynta shaqada endocrine pituitary. Dhammaan muunada togan waa in la xaqiijiyo by hababka kale. Baaritaankan waxaa loogu talagalay in loo isticmaalo xirfadlayaasha daryeelka caafimaadka oo keliya.
KOOBAN
Hormoonka Luteining (LH) waa glycoprotein leh miisaan kelli ah oo ku saabsan 30,000 Dalton, kaas oo ay soo saarto pituitary hore. Isku-duubnaanta LH waxay si dhow ula xiriirtaa ugxan-sidaha ugxan-sidaha, iyo heerka ugu sarreeya ee LH waxaa la saadaaliyay inay noqon doonto 24 ilaa 36 saacadood oo ugxansi ah. Sidaa darteed, qiimaha ugu sarreeya ee LH waa la kormeeri karaa inta lagu jiro wareegga caadada si loo go'aamiyo wakhtiga ugu fiican ee rimidda. Shaqada endocrine ee aan caadiga ahayn ee qanjidhada pituitary waxay sababi kartaa dheecaanka LH oo aan caadi ahayn. Xoogga saarista LH waxaa loo isticmaali karaa in lagu qiimeeyo shaqada qanjirka 'pituitary endocrine'. Xirmada ogaanshaha waxa ay ku salaysan tahay immunochromatography oo ku siin kara natiijo 15 daqiiqo gudahood.
MABADA HABKA
Xuubabka aaladda waxa lagu dahaadhay antibody LH ee gobolka tijaabada iyo ariga ka hortagga bakaylaha IgG ee gobolka kantaroolka. suufka suufka ah waxaa dahaarka leh fluorescence oo ku calaamadsan antibody LH iyo bakayle IgG ka hor. Marka la tijaabiyo muunada togan, antigen-ka LH ee muunada ku jira waxa uu ku daraa fluorescence lagu calaamadiyay antibody LH, oo waxa ay sameeyaan isku dhafka difaaca. Under tallaabada immunochromatography ah, socodka kakan ee jihada warqad nuugista, marka adag maray gobolka imtixaanka, waxaa la isku daray antibody LH daahan, foomamka adag cusub. Heerka LH waxa uu si togan ugu xidhan yahay calaamada fluorescence, iyo fiirsashada LH ee muunada waxa lagu ogaan karaa baadhitaanka difaaca jirka ee fluorescence.
AALADAHA IYO AALADAHA LA BIXIYAY
25T qaybaha xirmo:
.Kaarka imtixaanku si gooni gooni ah bireed ugu xidhan yahay 25T
.Sample diluents
.Baakadaha gelin
AALADAHA LOO BAAHAN YAHAY LAAKIIN AAN LA BIXIN
Tusaalaha weelka aruurinta, saacada
Tusaalaha Uruurinta iyo Kaydinta
1.Samballada la tijaabiyay waxay noqon karaan serum, heparin anticoagulant plasma ama EDTA plasma anticoagulant plasma.
2.Sida laga soo xigtay farsamooyinka caadiga ah ururiyaan muunad. Saambalka serum ama balaasmaha waxa lagu hayn karaa qaboojiyaha 2-8 ℃ muddo 7 maalmood ah iyo ilaalinta qaylada oo ka hooseeya -15°C muddo 6 bilood ah.
3.Dhammaan muunada iska ilaali wareegyada barafowga.
HABKA ASSAY
Habka tijaabada ee qalabka eeg buug-yaraha difaaca jirka. Habka tijaabada reagent waa sida soo socota
1. Geli dhammaan reagen-yaasha iyo shaybaarada ilaa heerkulka qolka.
2.Fur la qaadi karo Immune Analyzer (WIZ-A101), geli lambarka sirta ah ee xisaabta sida habka hawlgalka ee qalabka, oo geli interface ogaanshaha.
3.Scan code aqoonsiga si loo xaqiijiyo shayga tijaabada.
4.Ka soo saar kaadhka tijaabada bacda qashinka.
5. Geli kaadhka tijaabada booska kaadhka, iskaan koodhka QR, oo go'aami shayga tijaabada.
6.Ku dar 20μL serum ama muunad balasma si muunad dareere ah, oo si fiican isku qas.
7.Ku dar xal muunad 80μL si aad muunad fiican u hesho kaadhka.
8. Guji badhanka "tijaabada caadiga ah", 15 daqiiqo ka dib, qalabku wuxuu si toos ah u ogaan doonaa kaarka imtixaanka, wuxuu akhrin karaa natiijooyinka shaashadda qalabka, oo duubo / daabac natiijooyinka imtixaanka.
9.Tixraac tilmaamaha Falanqeeyaha difaaca jirka ee la qaadi karo (WIZ-A101).
Natiijooyinka Imtixaanka iyo Fasiraadda
Marxalada | Qiyaasta (mIU/ml) | |
Lab | 1.50-9.25 | |
Dheddig | wajiga follicular | 1.25-11.80 |
| muddada ugxansidaha | 13.15-94.75 |
| Marxaladda luteal | 1.05-14.50 |
| Menopause | 7.70-64.20 |
Xogta kor ku xusan waa muddada tixraaca ee loo dejiyay xogta lagu ogaanayo xirmadan, waxaana la soo jeediyay in shaybaar kastaa sameeyo waqti tixraac ah oo ku saabsan muhiimada caafimaad ee ku habboon dadka gobolkan.
.Foojinta LH way ka sarraysaa qiyaasta tixraaca, iyo isbeddelada jireed ama jawaab celinta diiqada waa in laga saaraa. Runtii aan caadi ahayn, waa in la isku daraa ogaanshaha calaamadaha bukaan-socodka.
Natiijooyinka habkan ayaa kaliya lagu dabaqi karaa tirada tixraaca ee lagu dejiyay habkan, natiijaduna si toos ah ulama barbar dhigi karto hababka kale.
Qodobada kale waxay sidoo kale keeni karaan khaladaad natiijooyinka ogaanshaha, oo ay ku jiraan sababo farsamo, khaladaad hawleed iyo arrimo kale oo muunad ah.
Kaydinta iyo Xasiloonida
1.Qalabka waa 18 bilood oo cimri dherer ah laga bilaabo taariikhda la soo saaray. Ku kaydi xirmooyinka aan la isticmaalin 2-30°C. HA BAFAYN. Ha isticmaalin wixii ka dambeeya taariikhda dhicitaanka.
2.Ha furin boorsada xidhan ilaa aad diyaar u tahay inaad samayso imtixaan, waxaana la soo jeediyay tijaabada hal-isticmaalka in lagu isticmaalo deegaanka loo baahan yahay (heerkulka 2-35 ℃, huurka 40-90%) 60 daqiiqo gudahood sida ugu macquulsan.
3.Sample diluent waxaa loo isticmaalaa isla markiiba ka dib marka la furo.
DIGNIIN IYO DIGTOONAADIN
Xirmada waa in la xidhaa lagana ilaaliyaa qoyaanka.
.Dhammaan shaybaarrada togan waa in lagu ansixiyo habab kale.
.Dhammaan shaybaarrada waa in loola dhaqmaa sidii kuwo wasakhaysan.
.HA isticmaalin reagen dhacay.
. Aan isku bedelin reajentiyeyaasha xirmooyinka leh tiro badan oo kala duwan.
Dib ha u isticmaalin kaadhadhka tijaabada iyo agabka la tuuri karo.
Xumaan-xumo, muunad xad-dhaaf ah ama yar waxay keeni kartaa weecsanaan natiijada.
LKU DAYDA
Si la mid ah baaritaan kasta oo isticmaalaya unugyada difaaca jirka ee jiirka, suurtogalnimada ayaa jirta faragelinta unugyada ka hortagga jiirka ee aadanaha (HAMA) ee muunadda. Noocyada bukaannada helay diyaarinta unugyada difaaca jirka ee monoclonal si loo baaro ama daawaynta waxaa ku jiri kara HAMA. Tijaabooyinka noocan oo kale ah waxay keeni karaan natiijooyin togan ama been abuur ah.
.Natiijooyinka baaritaankan ayaa kaliya loogu talagalay tixraaca bukaan-socodka, waa inaysan u adeegin aasaaska kaliya ee ogaanshaha iyo daaweynta bukaan-socodka, maamulka bukaan-socodka bukaan-socodka waa in ay noqdaan kuwo tixgelin ballaaran leh oo ay weheliyaan calaamadaha, taariikhda caafimaadka, baaritaanka shaybaarka kale, jawaab-celinta daaweynta, cudurrada faafa iyo macluumaadka kale. .
Reagent-kan waxa kaliya loo isticmaalaa baadhitaanada serum iyo balasmaha. Waxaa laga yaabaa inaysan helin natiijo sax ah marka loo isticmaalo muunado kale sida candhuufta iyo kaadida iyo iwm.
Tilmaamaha Waxqabadka
Linearity | 10mIU/ml ilaa 10000mIU/ml | leexashada qaraabo: -15% ilaa +15%. |
Isku xidhka tooska ah:(r)≥0.9900 | ||
Saxnaanta | Heerka soo kabashada waa in ay ahaataa 85% -115%. | |
Ku celcelinta | CV≤15% | |
Gaar ahaan (Mid ka mid ah walxaha ku jira faragelinta la tijaabiyay ma faragelin baaritaanka) | Faragelin | Isku-duubnida dhex-galka ah |
Hemoglobin | 200μg/ml | |
wareejinta | 100μg/ml | |
Dabocase fardaha peroxidase | 2000μg/ml | |
FSH | 200mIU/ml | |
HCG | 20000mIU/ml | |
TSH | 200μIU/ml |
TIXRAACYADA
1.Hansen JH, iyo al.HAMA Faragelinta Murine Monoclonal Antibody-based Immunoassays[J].J ee Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Dabeecada Heterophilic Antibodies iyo Doorka Faragelinta Immunoassay[J].J ee Clin Immunoassay,1992,15:108-114.
Furaha calaamadaha la isticmaalay:
In Vitro Diagnostic Device Medical | |
Soo saaraha | |
Ku kaydi at 2-30 ℃ | |
Taariikhda dhicitaanka | |
Dib ha u isticmaalin | |
TAXADIR | |
La tasho Tilmaamaha Isticmaalka |
Hal qayb oo ka mid ah Xiamen Wiz Biotech Co.,LTD
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