Qalabka baarista ee loo yaqaan 'isoenzyme mb of creation'(Iftiirence Impatoramograprogromatogrogantic-ka)
Loogu talagalay isticmaalka cudurka vitro? Kaliya
Fadlan si taxaddar leh u aqri xirmadan xirmadan ka hor intaadan isticmaalin oo si adag u raac tilmaamaha. Isku halaynta natiijooyinka Assay lama dammaanad qaadi karo haddii ay jiraan wax ka soo horjeedda oo ka socda tilmaamaha ku jira hagitaan.

Adeegsiga loogu talagalay
Xirmooyinka baarista ee ISOENENESME MB ee Kineine Kinase (Imporescescescescescomenogromatogratogratogrogromatogration) ee loo yaqaan 'smul-yareeynta' IsienesMomat 'of the plasmentation-ka (CK-MB) ee kumbuyuutarka (CK-MB) ee Amournice Kinather (CK-MB) ee loo yaqaan' Sermase's Service (CK-MB) ee Ambenice Kinather (CK-MB) ee loo yaqaan 'Aixirian' Insuro). Tirada togan oo dhami waa in lagu xaqiijiyaa habab kale. Tijaabadani waxaa loogu talagalay in lagu isticmaalo xirfad-yaqaanka daryeelka caafimaadka oo keliya.

Kooban
Heerarka CK-MB ayaa kordhay 4-6 saacadood ka dib markii jirkoolka myocardial uu dhacay, 18-24 saacadood, oo lagu soo celiyay 2-3 maalmood ka dib. CK-MB Siideynta wareegga mar dambe oo ka dib Hemoglobin.ck-MB waa sumadeeyaha dhaqanka ee Infaker-ka aasturdial

Mabda'a qalliinka
Xuubka aaladda tijaabada ah waxaa lagu daboolaa anti-MB ka hortagga maandooriyaha gobolka iyo ariga ari bakayle anti bakayle ee ka hortagga kontaroolka. Padble Da 'waxaa ku dahaadhay flurescience sumadeeya antibody ant igritody iyo bakayle ka hor. Markaad tijaabinayso muunad wanaagsan, ck-mb antigen ee antigen sahlanka lagu daro astaanta lagu calaamadeeyay antibito-ka ee ka hortagga ah ee anti's Mb, oo sameysa isku-darka difaaca. Iyada oo la raacayo ficilka tallaalka is-tallaalidda, qulqulka adag ee jihada warqad galka ah, marka isku dhafan uu ka gudbiyo gobolka tijaabada, waxaa lagu daraa ka hortagga ka hortagga ka hortagga daawada ka hortagga ah, oo sameysma isku dhafan cusub. Heerka CK-MB wuxuu si torerescience ku xidhnaa oo si torerescience ah, iyo uruurinta CK-MB ee muunad waxaa lagu ogaan karaa Impanossassay.

Reages iyo alaabada la keenay

25t qaybo xirmo ah:
Kaarka tijaabada shaqsi ahaan waxaa loo iibiyaa food-jeexjeex ah
Tusaalayaal muunad ah 25t
Xirmo geli 1

Qalabka loo baahan yahay laakiin lama bixin
Saamiga aruurinta tusaalaha, saacad

Ururinta muunad iyo kaydinta
1. Saamballada la tijaabiyay waxay noqon karaan Serum, Heparin lidka ku daaweynaya plasma ama edta lidka ku ah plasma plasma.
2.Coring in farsamooyinka caadiga ah ee aruurinta muunad. Muunadda Plasma ama Plasma Muunad waxaa lagu hayn karaa qaboojiyaha 2-8 ℃ 7 maalmood iyo mid ka yar oo ka hooseeya --15 ° C muddo 6 bilood ah.
3. Muunad ka fogow wareegyada baraf-qaboojiye.

Habka Assay
Fadlan aqri qalabka hawlgalka qalabka iyo xirmada xirmada ka hor baaritaanka.
1.Waxaan dhinac uga dhigin dhammaan reages iyo tusaalooyinka heerkulka qolka.
2.OPen falanqeeyaha difaaca ee la qaadan karo (Wiz-A101), gali furaha koontada furaha sirta ah iyadoo loo eegayo habka qalliinka ee qalabka, oo geli interface-ka ogaanshaha.
3.San lambarka ilkaha si loo xaqiijiyo sheyga imtixaanka.
4. Kaarka imtixaanka ka qaad bacda foil.
5. Kaarka tijaabada ee kaarka imtixaanka kaararka kaararka, iskaanka lambarka QR, oo go'aamiso sheyga imtixaanka.
5.DDD 40μl Serum ama Muunad Plasma ah oo muunad muunad ah, oo si wanaagsan isku dara ..
6.DDD 80μl muunad muunad ah oo muunad kaadhka looga qaado.
7. Badhanka "Imtixaanka 'Standard'.
8.Refer tilmaamaha falanqeynta cududda la qaadan karo (Wiz-A101).

Qiimaha la filayo
Ck-mb <5ng / ml
Waxaa lagu talinayaa in sheybaar kasta uu dhisi karo noocyo u gaar ah oo matalaya dadka bukaankeeda.

Natiijooyinka Tijaabada iyo Fasiraynta
. Xogta kore waa natiijada imtixaanka CK-MB Reacent, waxaana la soo jeedinayaa in shey kasta oo kasta ay tahay in la dhiso qiimayaasha ogaanshaha ee CK-MB ee ku habboon dadweynaha gobolkan. Natiijooyinka kor ku xusan waxaa loogu talagalay tixraac kaliya.
. Natiijooyinka habkan ayaa lagu dabaqi karaa oo keliya heerarka tixraaca ee lagu aasaasay qaabkan, oo ma jiro isbarbardhig toos ah oo lala galo habab kale.
. Waxyaabaha loo baahan yahay ayaa sidoo kale sababi kara khaladaad ku saabsan natiijooyinka ogaanshaha, oo ay ku jiraan sababo farsamo, khaladaad hawlgal iyo arrimo kale oo muunad ah.

Kaydinta iyo xasilloonida
1.Waxyada xirmada waa 18 bilood shelf-nolosha laga soo bilaabo taariikhda wax soo saarka. Ku keydi xirmooyinka aan la isticmaalin ee 2-30 ° C. Ha qaboojin. Ha u isticmaalin wixii ka dambeeya taariikhda dhicitaanka.
2. Ha furin jeebka shaabadaysan ilaa aad diyaar u tahay inaad tijaabiso, imtixaanka kaliya ee loo adeegsado waxaa loo soo jeedinayaa in loo adeegsado jawiga loo baahan yahay (heerkulka 2-35 humid, huurka 40-90%) 60 mins sida ugu dhakhsaha badan sida ugu macquulsan.
3.sample khayaano ayaa isla markiiba loo istcimaalaa kadib markii la furay.

Digniinta iyo taxadarka
.Then waa in la xirnaadaa lagana ilaaliyaa qoyaanka.
.All positive specimens shall be validated by other methodologies.
.Sital Tijaabada loo qaado waxaa loola dhaqmayaa sidii wasakheynta suuragalka ah.
.DO NOT use expired reagent.
.DO NOT interchange reagents among kits with different lot No..
.Do Dib u isticmaal kaararka tijaabada iyo waxyaabaha kasta oo la tuuro.
.Misoperation, excessive or little sample can lead to result deviations.

LIska yeelyeel
.As with any assay employing mouse antibodies, the possibility exists for interference by human anti-mouse antibodies (HAMA) in the specimen. Muunadaha ka imanaya bukaannada ka helay diyaarinta unugyada monoclonal-ka monoclonal ee loogu talagalay ogaanshaha cudurka ogaanshaha ama cudurka daweynta ayaa ku jiri kara Haa. Muunadaha noocan oo kale ah waxay sababi karaan natiijo been abuur ah ama natiijo diidmo ah oo been ah.
Natiijada baaritaanka ayaa kaliya loogu talagalay tixraaca caafimaad, ma aha inay u noqoto aasaaska ogaanshaha kiliinikada iyo daaweynta, bukaannadu waa inay la tixgeliyaan astaamaha, baaritaanka shaybaarka, baaritaanka shaybaarka, jawaabta daaweyn, epidemiology iyo macluumaad kale. .
.This reagent is only used for serum and plasma tests. Waxaa laga yaabaa inaysan helin natiijo sax ah marka loo isticmaalo shaybaarka kale sida candhuufta iyo kaadida iyo iwm.

Astaamaha waxqabadka

RJoonaansho
1.Hansen JH, et al.maware
2.Levinson Ss.Sida nooca unugyada ka hortagga ah ee unugyada ka hortagga ah iyo doorka faragalinta IvanoSassay [J].

Furaha calaamadaha la isticmaalay:

	Qalabka caafimaadka ee cudurka vitro?
	Wax soo saare
	Ku keydi 2-30 ℃
	Taariikhda uu dhacayo
	Dib ha u isticmaalin
	Taxadar
	Kala tasho tilmaamaha isticmaalka

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