Qalabka ogaanshaha eeXasaasiyadda C-reactive protein

(qiimaynta immunochromatographic fluorescence)

Isticmaalka ogaanshaha in vitro ee kaliya

Fadlan si taxadar leh u akhri xidhmadan xidhmadan ka hor intaanad isticmaalin oo si adag u raac tilmaamaha. Kalsoonida natiijooyinka qiimaynta lama dammaanad qaadi karo haddii ay jiraan wax ka leexasho ah tilmaamaha ku jira xidhmadan.

ISTICMAALKA KULAN

Qalabka ogaanshaha ee borotiinka C-reactive-ka kacsan (fluorescence immunochromatographic assay) waa shaybaarka fluorescence immunochromatographic ee lagu ogaanayo tirada borotiinka C-reactive (CRP) ee serum bini'aadamka /plasma/ dhiiga oo dhan. Waa tilmaame aan gaar ahayn oo barar ah. Dhammaan muunada wanaagsan waa in lagu xaqiijiyo habab kale. Baaritaankan waxaa loogu talagalay in loo isticmaalo xirfadlayaasha daryeelka caafimaadka oo keliya.

KOOBAN

borotiinka C-reactive waa borotiinka wejiga degdega ah oo ay soo saarto kicinta lymphokine ee beerka iyo unugyada epithelial. Waxay ku jirtaa serum bini'aadamka, dareeraha cerebrospinal, dareeraha xuubka iyo caloosha, iwm., waana qayb ka mid ah habka difaaca ee aan gaarka ahayn. 6-8h ka dib markii ay dhacdo infekshanka bakteeriyada, CRP waxay bilowday inay kordhiso, 24-48h waxay gaartay meeshii ugu sarreysay, qiimaha ugu sarreeyana wuxuu gaari karaa boqollaal jeer oo caadi ah. Ka dib markii la ciribtiray caabuqa, CRP aad ayuu hoos ugu dhacay wuxuuna ku soo noqday caadi hal toddobaad gudaheed. Si kastaba ha ahaatee, CRP aad uma korodho xaaladda caabuqa fayraska, kaas oo bixiya aasaaska aqoonsiga noocyada hore ee cudurrada, waana qalab lagu aqoonsanayo caabuqyada fayraska ama bakteeriyada.

MABADA HABKA

Xuubabka aaladda waxa lagu dahaadhay antibody CRP oo ku yaal gobolka tijaabada iyo ariga ka hortagga bakaylaha IgG ee gobolka kantaroolka. suufka suufka ah waxaa lagu dahaadhay fluorescence oo ku calaamadsan antibody CRP iyo bakayle IgG ka hor. Marka la tijaabiyo muunada togan, antigen-ka CRP ee muunada ku jira waxa uu ku daraa fluorescence calaamadsan antibody-ka CRP, oo sameeya isku dhafka difaaca jirka. Under tallaabada immunochromatography ah, socodka kakan ee jihada warqad nuugista, marka adag maray gobolka imtixaanka, waxaa la socda antibody daahan anti CRP, foomamka adag cusub. Heerka CRP wuxuu si togan ugu xidhan yahay calaamada fluorescence, iyo fiirsashada CRP ee muunada waxaa lagu ogaan karaa baaritaanka tallaalka fluorescence.