PRINCIPLE

Iyo tambo ine anti cal coating McAb pane bvunzo dunhu uye mbudzi anti-tsuro IgG antibody panzvimbo yekudzora, iyo yakasungirirwa kune membrane chromatography pamberi. Lable pad yakaputirwa negoridhe rekolloidal rakanyorwa kuti anti cal McAb uye colloidal goridhe rakanyorwa rebbit IgG antibody pamberi. Paunenge uchiyedza sampuli yakanaka, iyo cal mumuenzaniso yakasanganiswa negoridhe colloidal yakanyorwa anti cal McAb, uye inogadzira immune yakaoma, sezvo ichitenderwa kutama ichitevedza bvunzo tambo, iyo cal conjugate complex inobatwa ne anti cal coating McAb pa membrane uye fomu. "anti cal coating McAb-cal-colloidal goridhe yakanyorwa kuti anti cal McAb" yakaoma, bhendi rekuedza rine ruvara rakaonekwa munzvimbo yekuyedza. Iko kusimba kwemavara kunoenderana zvakanaka necal content. Sample yakashata haiburitse bhendi rekuyedza nekuda kwekushaikwa kwecolloidal goridhe conjugate cal complex. Hazvina mhosva kuti cal iripo mumuenzaniso kana kwete, kune mutsara mutsvuku unoonekwa padunhu rereferenzi uye dunhu rekutonga, iro rinoonekwa semhando yemukati bhizinesi zviyero.

Test Procedure:

1.Bvisa sampling stick, wopinzwa mune tsvina sampuli, wozodzorera sampling stick, screw tight uye zunza zvakanaka, dzokorora chiito katatu. Kana kushandisa sampling stick yanhonga 50mg tsvina yesampuli, woisa mutsvina yemuchubhu ine sample dilution, wobva wanyatsokukora.

2. Shandisa sampling yepipette inoraswa tora sampuli yetsvina yakatetepa kubva kumurwere wemanyoka, wobva wawedzera madonhwe matatu (angangoita 100uL) pachubhu yefecal sampling uye zunza zvakanaka, isa parutivi.

3.Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.

4.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera 4.drops (inenge 100uL) hapana bubble diluted sample verticaly uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi nekupa dispette, tanga nguva.

5.Chigumisiro chinofanira kuverengwa mukati memaminitsi e10-15, uye haibvumirwi mushure memaminitsi gumi nemashanu.