PRINCIPLE

Iyo tambo ine MP-Ag yekubikira antigen pane bvunzo dunhu uye mbudzi anti mbeva IgG antibody pane yekudzora dunhu, iyo yakasungirirwa kune membrane chromatography pamberi. Lable pad yakaputirwa negoridhe colloidal yakanyorwa mbeva-anti munhu IgM McAb pamberi. Paunenge uchiyedza sampuli yakanaka, iyo MP-IgM mumuenzaniso inosanganisa negoridhe colloidal yakanyorwa mbeva-anti yemunhu IgM McAb, uye inoita immune yakaoma. Pasi pekuita kweiyo immunochromatography, iyo yakaoma uye sampuli mukati meiyo nitrocellulose membrane inoyerera ichienda kune inonwisa bepa, iyo yakaoma payakapfuura dunhu rekuyedzwa, yakasanganiswa neMP-Ag yekuvhara antigen, ichigadzira "MP-Ag coating antigen-MP. -IgM-colloidal goridhe rakanyorwa mbeva-anti munhu IgM McAb” yakaoma, bhendi remavara rekuyedza rakaonekwa padunhu rebvunzo. Sample yakashata haiburitse bhendi rekuyedza nekuda kwekushomeka kweimmune immune complex. Hazvina mhosva kuti MP-IgM iripo mumuenzaniso kana kwete, kune mutsara mutsvuku unoonekwa padunhu rekutonga, iro rinoonekwa semhando yemukati bhizinesi zviyero.

Test Procedure:

Iyo WIZ-A101 bvunzo maitiro ona rairo yeinotakurika immune analyzer. Visual test maitiro ndeokuti:

1. Isa parutivi zvese reagents uye samples kune tembiricha yekamuri.
2. Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.
3. Wedzera 10μL serum kana plasma sample kana 20μL yese yeropa samples kuti uenzanise zvakanaka chekadhi nedispette yakapihwa, wobva wawedzera 100μL (inenge 2-3 donhwe) sample diluent, tanga nguva.
4. Mirira zvishoma 10-15 maminitsi uye uverenge chigumisiro, chigumisiro hachina kukodzera mushure memaminitsi gumi nemashanu.