PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neBoka A uye adenovirus antigen padunhu rekuyedza uye mbudzi anti tsuro IgG antibody padunhu rekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti Group A uye adenovirus uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka yeBoka A uye adenovirus, Boka A uye adenovirus mumuenzaniso zvinosanganiswa nefluorescence yakanyorwa kuti anti Rotavirus Boka A uye adenovirus, uye vogadzira immune musanganiswa. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ichienda kune inonwisa bepa. Kana yakaoma ikapfuura dunhu rekuyedzwa, yakasanganiswa neAnti-Rotavirus Boka A uye adenovirus yekuvhara antibody, inoumba nyowani yakaoma. Kana zvisina kunaka, hapana Rotavirus Group A uye adenovirus antigen mumuenzaniso, kuitira kuti zvirwere zvekudzivirira zvirwere zvigone kuumbwa, hapazovi nemutsara mutsvuku munzvimbo yekuona (T). Zvisinei nekuti boka A rotavirus uye adenovirus iripo mumuenzaniso, iyo latex-yakanyorwa mbeva IgG ine chromatographed kune yemhando yekudzora nzvimbo (C) uye inotorwa nembudzi anti-mouse IgG antibody. Mutsara mutsvuku uchaonekwa munzvimbo yekutonga kwehutano (C). Mutsara mutsvuku ndiwo chiyero chinoonekwa munharaunda yekudzora yemhando (C) yekutonga kana paine masampuli akakwana uye kana iyo chromatography process yakajairika. Inoshandiswawo seyemukati yekudzora chiyero che reagents.

Test Procedure:

1. Vhura chivharo chechubhu yekuunganidza sampuli. Usadira mushonga mubhodhoro.
2. Bvisa kamuti kesampling, wopinzwa mutsvina (kana shandisa sampling stick kuti utore 50mg yetsvina), wozodzorera sampling stick, screw tight uye zunza zvakanaka, dzokorora chiito katatu. Tora chikamu chakasiyana chesample yetsvina nguva yega yega. Mushure mekuita sampling, isa sampling rod muchubhu yekuunganidza tsvina ine sample diluent, uye nekukoira donhodzo zvakasimba. Kana tsvina yemurwere wemanyoka yakatetepa, sadza repurasitiki rinoraswa rinogona kushandiswa kuita sample. Uchishandisa sampling yepipette inoraswa tora sampuli yetsvina yakatetepa kubva kumurwere wemanyoka, wobva wawedzera madonhwe matatu (angangoita 100uL) pachubhu yefecal sampling.
3. Dhonza zvakanaka sampuli uye bvisa chivharo pane dropper tip uye woisa parutivi.
4. Kana yakachengetwa pakupisa kwakadzika, kititi inofanira kudzorerwa kune tembiricha yekamuri isati yashandiswa. Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.
5. Bvisa chivharo kubva kumuenzaniso wechubhu uye urase madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample vertically uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi nekupa dispette, tanga nguva.
6. Chigumisiro chinofanira kuverengwa mukati memaminitsi 10-15, uye hazvina kukodzera mushure memaminitsi gumi nemashanu.