PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neHp-Ab antibody padunhu rekuyedza uye mbudzi anti tsuro IgG antibody panzvimbo yekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti Hp-Ag uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka, iyo Hp-Ab mumuenzaniso inosanganisa nefluorescence yakanyorwa anti Hp-Ag, uye gadzira immune system. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ichienda kune inonwisa bepa. Kana yakaoma ikapfuura dunhu rekuyedzwa, yakasanganiswa neAnti Hp-Ag yekuvhara antibody, inoumba nyowani yakaoma. Kana zvisina kunaka, hapana HP antibody mumuenzaniso, kuitira kuti immune complexes irege kuumbwa, hapazovi nemutsara mutsvuku munzvimbo yekuona (T). Mutsara mutsvuku ndiwo chiyero chinoonekwa munharaunda yekudzora yemhando (C) yekutonga kana paine masampuli akakwana uye kana iyo chromatography process yakajairika. Inoshandiswawo seyemukati yekudzora chiyero che reagents.

Test Procedure:

Ndokumbira uverenge pasuru usati waedza.

1. Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.

2. Wedzera madonhwe maviri e serum kana plasma sample (kana madonhwe matatu eropa rose/munwe wemunwe) kuti uedze zvakanaka wekadhi nedispette yakapihwa, wobva wawedzera donhwe 1 resample diluent, tanga nguva.

3. Mirira zvishoma 10-15 maminitsi uye uverenge chigumisiro mumaminitsi 10-15. Mhedzisiro haishande mushure memaminitsi gumi nemashanu.