Vitamin D ivhitamini uye iriwo steroid hormone, kunyanya kusanganisira VD2 neVD3, ine kuraira kwakafanana. Vitamin D3 uye D2 inoshandurwa kuita 25 hydroxyl vhitamini D (kusanganisira 25-dihydroxyl vhitamini D3 uye D2). 25-(OH) VD mumuviri wemunhu, kuraira kwakagadzikana, kukwira kwakanyanya. 25-(OH) VD inoratidza huwandu hwevhitamini D, uye kukwanisa kutendeuka kwevhitamini D, saka 25-(OH) VD inoonekwa sechiratidzo chakanakisisa chekuongorora huwandu hwevhitamini D. The Diagnostic Kit inobva pa immunochromatography uye inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.