SUMMARY

Vitamin D ivhitamini uye iriwo steroid hormone, kunyanya kusanganisira VD2 neVD3, ine kuraira kwakafanana. Vitamin D3 uye D2 inoshandurwa kuita 25 hydroxyl vhitamini D (kusanganisira 25-dihydroxyl vhitamini D3 uye D2). 25-(OH) VD mumuviri wemunhu, kuraira kwakagadzikana, kukwira kwakanyanya. 25-(OH) VD inoratidza huwandu hwevhitamini D, uye kukwanisa kutendeuka kwevhitamini D, saka 25-(OH) VD inoonekwa sechiratidzo chakanakisisa chekuongorora huwandu hwevhitamini D. The Diagnostic Kit inobva pa immunochromatography uye inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

ZVINOTAURWA ZVINOITA

Iyo membrane yechishandiso chekuyedza yakavharwa neiyo conjugate yeBSA uye 25-(OH) VD padunhu rekuyedza uye mbudzi anti tsuro IgG antibody padunhu rekutonga. Marker pad akaputirwa nefluorescence mark anti 25-(OH) VD antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampuli, 25-(OH) VD mumuenzaniso sanganisa nefluorescence yakanyorwa anti 25-(OH)VD antibody, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, kuyerera kwakaoma munzira yepepa rinonyudza, apo yakaoma yakapfuura dunhu rekuyedzwa, Iyo yemahara fluorescent marker ichabatanidzwa ne 25-(OH) VD pa membrane.The concentration ye25-(OH) VD haina kunaka kuwirirana kweiyo fluorescence chiratidzo, uye kusungirirwa kwe25-(OH) VD mumuenzaniso kunogona kuonekwa nefluorescence immunoassay assay.