PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neRotavirus Boka A antigen munzvimbo yekuyedza uye mbudzi anti tsuro IgG antibody panzvimbo yekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti Rotavirus Boka A uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka, iyo RV mumuenzaniso inosanganisa nefluorescence yakanyorwa anti Rotavirus Boka A, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ichienda kune inonwisa bepa. Kana yakaoma ikapfuura dunhu rekuyedzwa, yakasanganiswa neAnti-Rotavirus Boka A yekuputira antibody, inoumba nyowani yakaoma. Kana zvisina kunaka, hapana Rotavirus Group A antigen mumuenzaniso, kuitira kuti zvirwere zvekudzivirira zvirwere zvirege kuumbwa, hapazovi nemutsara mutsvuku munzvimbo yekuona (T). Zvisinei nekuti boka A rotavirus riripo here mumuenzaniso, iyo latex-yakanyorwa mbeva IgG yakadhirowewa kune yemhando yekudzora nzvimbo (C) uye inobatwa nembudzi anti-mouse IgG antibody. Mutsara mutsvuku uchaonekwa munzvimbo yekutonga kwehutano (C). Mutsara mutsvuku ndiwo chiyero chinoonekwa munharaunda yekudzora yemhando (C) yekutonga kana paine masampuli akakwana uye kana iyo chromatography process yakajairika. Inoshandiswawo seyemukati yekudzora chiyero che reagents.

Test Procedure:

1.Symptomatic varwere vanofanira kuunganidzwa. Maererano nemishumo, iyo yakawanda excretion ye rotavirus mumarara evarwere vane gastroenteritis inoitika mazuva 3-5 mushure mekutanga kwechirwere uye mazuva 3-13 mushure mekutanga kwezviratidzo. Kana sampuli yakaunganidzwa kwenguva refu mushure mekuita manyoka, nhamba yemaantigen inogona kunge isina kukwana kuti iite maitiro akanaka.

2.Mienzaniso inofanira kuunganidzwa mumudziyo wakachena, wakaoma, usina mvura usina mishonga yekuchenesa uye inochengetedza.

3.Kune varwere vasina manyoka, masampuli etsvina akaunganidzwa haafaniri kunge ari pasi pe1-2 gramu. Kune varwere vane manyoka, kana tsvina iri mvura, ndapota tora 1-2 ml yetsvina yemvura. Kana tsvina iine ropa rakawanda nemako, ndapota tora sampuli zvakare.

4.Inokurudzirwa kuedza sampuli pakarepo mushure mekuunganidza, kana zvisina kudaro inofanira kutumirwa ku laboratori mukati maawa matanhatu uye kuchengetwa pa 2-8 ° C. Kana sampuli dzisina kuongororwa mukati memaawa makumi manomwe nemaviri, dzinofanirwa kuchengetwa patembiricha iri pasi -15°C.

5.Shandisa tsvina itsva yekuongorora, uye tsvina yesampuli yakasanganiswa nemvura yakasvibiswa kana yakasvibiswa