PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neFOB antibody padunhu reyedzo uye mbudzi anti tsuro IgG antibody padunhu rekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti FOB antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka, iyo FOB antigen mumuenzaniso inosanganisa nefluorescence yakanyorwa anti FOB antibody, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ichienda kune inonwisa bepa. Kana yakaoma ikapfuura dunhu rekuyedzwa, yakasanganiswa neanti FOB yekuvhara antibody, inoumba nyowani yakaoma. Kana zvisina kunaka, sampuli haina hemoglobin yevanhu kana kuti zvinyorwa zvakaderera, zvakakwana immune complexes haigoni kuumbwa. Pachave pasina mutsara mutsvuku munzvimbo yekuona (T). Mutsara mutsvuku ndiwo chiyero chinoonekwa munharaunda yekudzora yemhando (C) yekutonga kana paine masampuli akakwana uye kana iyo chromatography process yakajairika. Inoshandiswawo seyemukati yekudzora chiyero che reagents.

Test Procedure:

Ndokumbira uverenge pasuru usati waedza.

1.Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.

2.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample vertically uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi ine dipette yakapiwa. Zvadaro tanga timer.

3.Chigumisiro chinofanira kuverengwa mukati me10-15 maminitsi, uye hazvina kukodzera mushure memaminitsi gumi nemashanu.