Diagnostic Kit yeEstradiol (fluorescence immunochromatographic assay)
Diagnostic Kit yeEstradiol(fluorescence immunochromatographic assay)
Yekushandisa in vitro diagnostic chete
Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo. Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.
ZVIRI KUSHANDISA
Diagnostic Kit yeEstradiol (fluorescence immunochromatographic assay) ndeye fluorescence immunochromatographic assay yehuwandu hwekuonekwa kweEstradiol (E2) mu serum yevanhu kana plasma, iyo inonyanya kushandiswa kuongorora mazinga eEstradiol. inofanira kusimbiswa nedzimwe nzira. Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete.
SUMMARY
Estradiol (E2) ndiyo inonyanya kukosha uye inonyanya kushanda hormone mu estrogen.Iyo molecular weight is 272.3 D.Muzhinji, kune vakadzi vasina pamuviri, E2 inonyanya kuvharwa nesheath uye granular cells uye luteal cells panguva yekukura kwefollicular.Panguva yekuzvitakura, E2 inonyanya kubudiswa ne placenta, nepo varume vachinyanya kugadzirwa nemachende.Mushure meE2 yapinda muropa,1% kusvika 3% haisungiri kumapuroteni, 40% inosunga kuhomoni yebonde inosunga globulin(SHBG),uye vamwe vanosunga albumin, metabolize kubva pachiropa kupinda mumvura-soluble sulfates kana gluconaldehyde esters, uye yakabudiswa kubva muweti.E2 chiratidzo chehomoni inokosha yekuongorora kushanda kweovarian.
ZVINOTAURWA ZVINOITA
Iyo membrane yechishandiso chekuyedza yakavharwa neiyo conjugate yeBSA neEstradiol padunhu rekuyedza uye mbudzi anti tsuro IgG antibody padunhu rekutonga. Marker pad akaputirwa nefluorescence mark anti E2 antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampu, E2 mumuenzaniso inosanganisa nefluorescence yakanyorwa anti E2 antibody, uye gadzira immune system. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ikananga kune inonyudza bepa, apo yakaoma yakapfuura dunhu rekuyedzwa, Iyo yemahara fluorescent marker ichabatanidzwa neEstradiol pa membrane.Kusangana kweEstradiol ndiko kukanganisa kuwirirana kwechiratidzo chefluorescence, uye iyo kusangana kweEstradiol mumuenzaniso kunogona kuonekwa ne fluorescence immunoassay assay.
REAGENTS NEZVINHU ZVINOPIWA
25T package zvikamu:
.Kuedza kadhi rimwe nerimwe foil yakaiswa muhomwe ine desiccant 25T
.A mhinduro 25T
.B mhinduro 1
.Package isa 1
ZVINHU ZVINODIWA ASI ZVISINA KUPIWA
Sample yekuunganidza mudziyo, timer
SAMPLE KUCHENGA UYE KUCHENGA
1.Mienzaniso yakaedzwa inogona kuva serum, heparin anticoagulant plasma kana EDTA anticoagulant plasma.
2.According to standard techniques unganidza sampuli. Serum kana plasma sampuli inogona kuchengetwa firiji pa 2-8 ℃ kwemazuva 7 uye cryopreservation pazasi -15 ° C kwemwedzi mitanhatu.
.Yese sampuli inodzivirira kutonhora-kunyungudika kutenderera.
ASAY PROCEDURE
Maitiro ekuyedza echiridzwa ona iyo immunoanalyzer bhuku. Iyo reagent test maitiro ndeaya anotevera
1.Kuisa parutivi zvose reagents uye samples kune tembiricha yekamuri.
2.Vhura iyo Portable Immune Analyzer (WIZ-A101), isa iyo password password login maererano nenzira yekushanda yechiridzwa, uye pinda iyo yekuona interface.
3.Scan dentification code kuti usimbise chinhu chekuedza.
3.Bvisa kadhi rekuedza kubva mubhegi refoil.
4.Isai kadhi rekuedza mukadhi kadhi, tarisai QR code, uye sarudza chinhu chekuedza.
5.Wedzera 30μL serum kana plasma sampuli kuA solution, uye sanganisa zvakanaka.
6.Wedzera 20μL B mhinduro kumusanganiswa wepamusoro, uye sanganisa zvakanaka.
Siya musanganiswa we20maminitsi.
Wedzera 80μL musanganiswa kuti uenzanise zvakanaka wekadhi.
Dzvanya bhatani re "standard test", mushure memaminitsi gumi, chiridzwa chinozongoona kadhi rebvunzo, chinogona kuverenga zvabuda kubva pachiratidziro chechiridzwa, uye kurekodha / kudhinda mhinduro dzebvunzo.
Tarisa kune rairo yePortable Immune Analyzer (WIZ-A101).
TEST RESULTS UYE DUDZIRIRO
| Danho | Range(pg/mL) | |
| Murume | 12.5-54.5 | |
| Mukadzi | follicular phase | 28.5-185 |
| ovulatory period | 81.5-408 | |
| Luteal phase | 40.5-272 | |
| Kuenda kumwedzi | 13.6-42.5 | |
.Iyo data iri pamusoro apa inguva yereferensi yakagadzwa ye data yekuona yekiti iyi, uye zvinokurudzirwa kuti laboratori yega yega inofanira kumisa nguva yereferensi yezvakakoshesesa zvekiriniki zvehuwandu hwevanhu munharaunda ino.
.Kuwanda kweEstradiol kwakakwirira kudarika mutsara wekutsvaga, uye kuchinja kwehupenyu kana kushushikana kwepfungwa kunofanira kubviswa.Ichokwadi chisina kujairika, chinofanira kusanganisa kuongororwa kwechiratidzo chekliniki.
.Migumisiro yeiyi nzira inongoshanda kune mutsara wekutsvaga wakagadzwa nenzira iyi, uye zvigumisiro hazvifananidzi zvakananga nedzimwe nzira.
.Zvimwe zvikonzero zvinogonawo kukonzera kukanganisa mumigumisiro yekuona, kusanganisira zvikonzero zvehutano, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.
KUCHENGA UYE KUgadzikana
1.Ikiti ndeye 18 mwedzi pasherufu-hupenyu kubva pazuva rekugadzirwa. Chengetedza makiti asina kushandiswa pa2-30°C. MUSAIZE. Usashandise kupfuura zuva rekupera.
2.Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo-yekushandisa imwe chete inokurudzirwa kushandiswa pasi penzvimbo inodiwa (tembiricha 2-35 ℃, humidity 40-90%) mukati me60 mins nekukurumidza. sezvinobvira.
3.Sample diluent inoshandiswa pakarepo mushure mekuvhurwa.
YAMBIRO UYE CHENJERERO
.Ikiti inofanira kuvharwa uye kuchengetedzwa kubva pakunyorova.
.Yese mienzaniso yakanaka ichasimbiswa nedzimwe nzira.
.Mienzaniso yese ichabatwa sezvingango svibisa.
.USA shandisa expired reagent.
.USACHINANA mareagents pakati pemakiti ane akasiyana lot no..
.USAshandise zvakare makadhi ebvunzo uye chero zvinhu zvinoraswa.
.Misoperation, yakawandisa kana sampuli shoma inogona kutungamirira kumhedzisiro yekutsauka.
LIMITATION
.Sezvinoita chero muyedzo unoshandisa masoja ekudzivirira embeva, mukana uripo wekukanganiswa nemasoja ekudzivirira mbeva (HAMA) mumuenzaniso. Mienzaniso kubva kuvarwere vakagamuchira gadziriro dzemonoclonal antibodies yekuongorora kana kurapwa inogona kunge iine HAMA. Mienzaniso yakadaro inogona kukonzera nhema dzakanaka kana nhema dzisina kunaka.
.Iyi mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete, haifanire kushanda seyo chete hwaro hwekuongororwa kwekiriniki uye kurapwa, varwere vekiriniki manejimendi inofanirwa kutariswa yakazara yakasanganiswa nezviratidzo zvayo,
.nhoroondo yezvechiremba, kumwe kuongorora kwerabhoritari, mhinduro yekurapa, epidemiology uye mamwe mashoko.
.Iri reagent rinoshandiswa chete serum uye plasma tests. Iyo inogona kusawana mhedzisiro chaiyo kana ichishandiswa kune mamwe masampuli senge mate uye weti uye nezvimwe.
PERFORMANCE CHARACTERISTICS
| Linearity | 30 pg/mL kusvika 2000 pg/mL | hama kutsauka: -15% kusvika +15%. |
| Linear coefficient yekubatanidza:(r)≥0.9900 | ||
| Kururama | Chiyero chekudzorera chichave mukati me85% - 115%. | |
| Kudzokorora | CV≤15% | |
| Zvakananga(Hapana chimwe chezvinhu pane chakakanganiswa chakaedzwa chakakanganisa muyedzo) | Kupindira | Kuvhiringidza kuisa pfungwa |
| T | 500ng/mL | |
| PROG | 500ng/mL | |
| VaK | 500ng/mL | |
| E3 | 100ng/mL | |
| 17β-E2 | 100ng/mL | |
REFERENCES
1.Hansen JH, et al.HAMA Kupindira neMurine Monoclonal Antibody-Based Immunoassays[J].J yeClin Immunoassay,1993,16:294-299.
2.Levinson SS.Mamiriro eHeterophilic Antibodies uye Basa muImmunoassay Interference[J].J yeClin Immunoassay,1992,15:108-114.
Kiyi yezviratidzo zvakashandiswa:
 | In Vitro Diagnostic Medical Device |
 | Mugadziri |
 | Chengetedza pa2-30 ℃ |
 | Zuva rekupera |
 | Usashandisezve |
 | KUCHENJERA |
 | Bvunza Mirayiridzo Yekushandisa |
Xiamen Wiz Biotech CO., LTD
Kero:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel:+86-592-6808278
Fax:+86-592-6808279
