Diagnostic Kit yeAntigen kune Helicobacter Pylori (Fluorescence Immunochromatographic Assay)
Diagnostic Kit yeAntigen kune Helicobacter Pylori(Fluorescence Immunochromatographic Assay)
Yekushandisa in vitro diagnostic chete
Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo. Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.
ZVIRI KUSHANDISA
Diagnostic Kit yeAntigen kusvika kuHelicobacter Pylori (Fluorescence Immunochromatographic Assay) inokodzera kuongororwa kwehuwandu hwetsvina yevanhu HP antigen nefluorescence immunochromatographic assay, iyo ine yakakosha yekuongororwa kukosha kwehutachiona hwegastric. Yese yakanaka sampuli inofanirwa kusimbiswa nedzimwe nzira. Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete.
SUMMARY
Gastric helicobacter pylori utachiona hune hukama hwepedyo nekusingaperi gastritis, gastric ulcer, gastric adenocarcinoma, gastric mucosa yakabatana lymphoma, Hp ylori hutachiona hwehuwandu hunosvika 90% mugastritis, gastric ulcer, duodenal ulcer uye gastric cancer varwere. Sangano reWorld Health Organisation raona h. pylori serudzi rwekutanga rwechirwere chinokonzera kenza uye zviri pachena kuti njodzi yekenza yemudumbu.H.H.pylori inowanikwa inokosha zvikuru mukuongororwa kweh. pylori infection.The test based on fluorescence immunochromatographic assay analysis technics, inogona kupa chigumisiro mukati memaminitsi gumi nemashanu.
ZVINOTAURWA ZVINOITA
Iyo tambo ine anti-HP yekuvhara antibody padunhu rebvunzo, iyo yakasungirirwa kune membrane chromatography pamberi. Lable pad yakaputirwa nefluorescence yakanyorwa anti-HP antibody pamberi. Paunenge uchiyedza sampuli yakanaka, iyo HP iri mumuenzaniso inogona kusanganiswa nefluorescence yakanyorwa kuti anti-HP antibody, uye kuumba immune system. Sezvo musanganiswa uchitenderwa kutama uchitevedza bvunzo strip, iyo HP conjugate complex inobatwa ne-anti-HP yekuvhara antibody pane membrane uye mafomu akaoma. Iyo fluorescence intensity inonyatso kuenderana neiyo HP yemukati. Iyo HP mumuenzaniso inogona kuonekwa ne fluorescence immunoassay analyzer.
REAGENTS NEZVINHU ZVINOPIWA
25T package zvikamu:
Kadhi rekuyedza rimwe nerimwe foil rakaiswa muhomwe ine desiccant 25T
Sample diluents 25T
Package isa 1
ZVINHU ZVINODIWA ASI ZVISINA KUPIWA
Sample yekuunganidza mudziyo, timer
SAMPLE KUCHENGA UYE KUCHENGA
1.Shandisa chigaba chakachena chinoraswa kuti utore sampuli yetsvina itsva, woongororwa nekukasika. Kana isingakwanisi kuongororwa nekukasira, ndapota inochengetwa pa2-8 ° C kwemazuva matatu kana pasi -15 ° C kwemwedzi mitanhatu.
2.Bvisa sampling stick, wopinza mutsvina, dzokorora chiito katatu, tora zvikamu zvakasiyana zvetsvina nguva yega yega, wozodzorera sampling stick, screw tight wozunza zvakanaka, Kana kushandisa sampling stick yanhongwa. nezve 50mg tsvina yesampuli, uye isa muchubhu yemuchubhu ine tsvina ine sample dilution, uye screw zvakasimba.
3.Shandisa pipette sampling inoraswa tora tsvina yemurwere wemanyoka, wobva wawedzera madonhwe matatu (angangoita 100µL) pachubhu yefecal sampling uye zunza zvakanaka.
Notes:
1.Dzivisa kutonhora-kunyungudika kutenderera.
2.Thaw samples kune tembiricha yekamuri usati washandisa.
ASAY PROCEDURE
Ndokumbira uverenge bhuku rekushandisa chekushandisa uye pasuru isa usati waedza.
1.Kuisa parutivi zvose reagents uye samples kune tembiricha yekamuri.
2.Vhura iyo Portable Immune Analyzer (WIZ-A101), isa iyo password password login maererano nenzira yekushanda yechiridzwa, uye pinda iyo yekuona interface.
3.Scan dentification code kuti usimbise chinhu chekuedza.
4.Bvisa kadhi rekuedza kubva mubhegi refoil.
5.Isai kadhi rekuedza mukadhi yekadhi, tarisai QR code, uye sarudza chinhu chekuedza.
6.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample verticaly uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi ine dipette yakapiwa.
7.Click "standard test" bhatani, mushure memaminitsi e15, chiridzwa chichangoona kadhi rekuedza, rinogona kuverenga migumisiro kubva pachiratidziro chechiratidzo chechiridzwa, uye nyora / kudhinda mhinduro dzekuedza.
8.Rejera kune rairo yePortable Immune Analyzer(WIZ-A101).
ZVINOTARISIRWA ZVINOKOSHA
HP-Ag <10
Zvinokurudzirwa kuti murabhoritari yega yega imise zera rayo rakajairwa rinomiririra huwandu hwevarwere vayo.
TEST RESULTS UYE DUDZIRIRO
1.Iyo HP-Ag mumuenzaniso inopfuura 10, uye inofanira kutonga kunze kwekuchinja kwehutano hwehupenyu. Mhedzisiro yacho zvechokwadi haina kujairika uye inofanirwa kuongororwa zviratidzo zvekiriniki.
2.Migumisiro yeiyi nzira inoshandiswa chete kune zvinyorwa zvinotaridzirwa zvakasimbiswa nenzira iyi, uye hapana kuenzaniswa kwakananga nedzimwe nzira.
3.Zvimwe zvikonzero zvinogonawo kukonzera kukanganisa mumigumisiro yekuona, kusanganisira zvikonzero zvehutano, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.
KUCHENGA UYE KUTSIGIRA
1.Ikiti ndeye 18 mwedzi pasherufu-hupenyu kubva pazuva rekugadzirwa. Chengetedza makiti asina kushandiswa pa2-30°C. MUSAIZE. Usashandise kupfuura zuva rekupera.
2.Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo-yekushandisa imwe chete inokurudzirwa kushandiswa pasi penzvimbo inodiwa (tembiricha 2-35 ℃, humidity 40-90%) mukati me60 mins nekukurumidza. sezvinobvira.
3.Sample diluent inoshandiswa pakarepo mushure mekuvhurwa.
YAMBIRO UYE CHENJERERO
.Ikiti inofanira kuvharwa uye kuchengetedzwa kubva pakunyorova.
.Yese mienzaniso yakanaka ichasimbiswa nedzimwe nzira.
.Mienzaniso yese ichabatwa sezvingango svibisa.
.USA shandisa expired reagent.
.USACHINANA mareagents pakati pemakiti ane akawanda akawanda Nha.
.USAshandise zvakare makadhi ebvunzo uye chero zvinhu zvinoraswa.
.Misoperation, yakawandisa kana sampuli shoma inogona kutungamirira kumhedzisiro yekutsauka.
LIMITATION
.Sezvinoita chero muyedzo unoshandisa masoja ekudzivirira embeva, mukana uripo wekukanganiswa nemasoja ekudzivirira mbeva (HAMA) mumuenzaniso. Mienzaniso kubva kuvarwere vakagamuchira gadziriro dzemonoclonal antibodies yekuongorora kana kurapwa inogona kunge iine HAMA. Mienzaniso yakadaro inogona kukonzera nhema dzakanaka kana nhema dzisina kunaka.
.Iyi mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete, haifanire kushanda seyo chete hwaro hwekuongororwa kwekiriniki uye kurapwa, varwere vekiriniki manejimendi vanofanirwa kutarisisa kwakadzama kusanganiswa nezviratidzo zvayo, nhoroondo yekurapa, imwe ongororo yerabhoritari, mhinduro yekurapa, epidemiology uye mamwe mashoko. .
.Iri reagent rinoshandiswa pakuongorora tsvina chete. Iyo inogona kusawana mhedzisiro chaiyo kana ichishandiswa kune mamwe masampuli senge mate uye weti uye nezvimwe.
PERFORMANCE CHARACTERISTICS
| Linearity | 10-1000 | hama kutsauka: -15% kusvika +15%. |
| Linear coefficient yekubatanidza:(r)≥0.9900 | ||
| Kururama | Chiyero chekudzorera chichave mukati me85% - 115%. | |
| Kudzokorora | CV≤15% | |
REFERENCES
1.Shao,JL&F.Wu.Kufambira mberi munguva pfupi yapfuura mukuona nzira dzeHelicobacter pylori[J].Journal of Gastroenterology and Hepatology,2012,21(8):691-694
2.Hansen JH, et al.HAMA Kupindira neMurine Monoclonal Antibody-Based Immunoassays[J].J yeClin Immunoassay,1993,16:294-299.
3.Levinson SS.Mamiriro eHeterophilic Antibodies uye Basa muImmunoassay Interference[J].J yeClin Immunoassay,1992,15:108-114.
Kiyi yezviratidzo zvakashandiswa:
 | In Vitro Diagnostic Medical Device |
 | Mugadziri |
 | Chengetedza pa2-30 ℃ |
 | Zuva rekupera |
 | Usashandisezve |
 | KUCHENJERA |
 | Bvunza Mirayiridzo Yekushandisa |
Xiamen Wiz Biotech CO., LTD
Kero:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel:+86-592-6808278
Fax:+86-592-6808279
