PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neiyo conjugate yeBSA uye 25-(OH) VD padunhu rekuyedza uye mbudzi anti tsuro IgG antibody padunhu rekutonga. Marker pad akaputirwa nefluorescence mark anti 25-(OH) VD antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampuli, 25-(OH) VD mumuenzaniso sanganisa nefluorescence yakanyorwa anti 25-(OH)VD antibody, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, kuyerera kwakaoma munzira yepepa rinonyudza, apo yakaoma yakapfuura dunhu rekuyedzwa, Iyo yemahara fluorescent marker ichabatanidzwa ne 25-(OH) VD pa membrane.The concentration ye25-(OH) VD haina kunaka kuwirirana kweiyo fluorescence chiratidzo, uye kusungirirwa kwe25-(OH) VD mumuenzaniso kunogona kuonekwa nefluorescence immunoassay assay.

Test Procedure:

Ndokumbira uverenge bhuku rekushandisa chekushandisa uye pasuru isa usati waedza.

1. Isa parutivi zvese reagents uye samples kune tembiricha yekamuri.
2. Vhura iyo Portable Immune Analyzer (WIZ-A101), isa iyo password password login maererano nenzira yekushanda yechiridzwa, uye pinda iyo yekuona interface.
3. Skena kodhi yedentification kuti usimbise chinhu chebvunzo.
4. Bvisa kadhi rebvunzo mubhegi refoiri.
5. Isai kadhi rekuedza mukadhi slot, tarisa QR code, uye sarudza chinhu chekuedza.
6. Wedzera 15μL serum kana plasma sampuli mumhinduro, uye sanganisa zvakanaka
7. Wedzera 80μL musanganiswa kuti uenzanise zvakanaka wekadhi.
8. Dzvanya bhatani re "standard test", mushure memaminitsi e15, chiridzwa chichangoona kadhi rekuedza, rinogona kuverenga zvabuda kubva pachiratidziro chechiridzwa, uye kurekodha / kudhinda mhinduro dzebvunzo.
9. Tarisa kune rairo yePortable Immune Analyzer (WIZ-A101).