Diagnostic kit (colloidal goridhe) ye calprodentin
Diagnostic kit(Colloidal Goridhe)for calprotectin
Nekuti mu vitro diagnostics chete
Ndokumbirawo kuti muverenge iyi package isa zvakachenjera usati washandisa uye nekuteerera zvakanyanyisa mirairo. Kuvimbika kweMhedzisiro Mhedzisiro haigone kuvimbiswa kana paine chero kutsauka kubva pamirairo mune ino package isa.
Yakatarisirwa kushandiswa
Diagnostic kit yeCalprotectin (Cal) iri collodal Gondiyo Iyi bvunzo ndeyekuchengetedzeka. Zvese zvakanaka sampuli inofanira kusimbiswa neimwe nzira. Iyi bvunzo inoitirwa hutano hwehutano hwekushandisa chete. Zvichakadaro, bvunzo iyi inoshandiswa yeIVD, zviridzwa zvekuwedzera hazvidiwe.
Pfupiso
Cal is heterodimer, iyo inoumbwa naVRP 8 uye MRP 14 Cal iri acute proteins, ine chikamu chakasimba chakagadzikana chinenge vhiki imwe muzvina zvevanhu, zvakatemwa kuve muchengeti ane hosha yechirwere. Iyo kit iri nyore, yekuona sequalitative bvunzo iyo inoona cal mune menzvimbo yevanhu, ine yakakwirira yekuona kunzwa uye kwakasimba kwakasimba. Muedzo wakavakirwa pane yakakwirira yakatarwa Sandhodies Sandwich Reaction Stuction uye Goridhe AceMunochromatographic fungidziro yehunyanzvi, inogona kupa mhedzisiro mukati memaminetsi gumi nemashanu.
Nheyo yehunhu
Iyo strip ine anti cal coating mcab pane yeTest Dunhu uye Goat Anti-Rabbit Igg Antibody mudunhu rekutonga, iyo yakasungirirwa kusvika kumambrane chromato zvisati zvaitika. Pad Lable Pad inobatanidzwa negoridhe yeLollodal yakanyorwa anti cal cal uye collodal goridhe yakanyorwa kurudziro yerababhi Igg antibody pamberi. Kana uchiedza sampuli yakanaka, iyo cal yakaumbwa negoridhe yeLollodal yakanyorwa antix. "Anti cal anokwereta mcab-cal-collidal goridhe yakanyorwa anti cal cal Iyo ruvara ruvara rwakanyanya kuenderana ne cal yezvinhu. Sampui isina kunaka haigadzi bhendi yebvunzo nekuda kwekusavapo kwe9lodal goridhe conjugate cal calx. Hapana mabasa cal aripo mumuenzaniso kana kuti kwete, pane mitsara tsvuku inoonekwa pane Reference Region uye mhando yekutonga, inoonekwa semhando yemukati yemukati bhizinesi.
Reagents uye zvinhu zvinopihwa
25T Package zvikamu:
.Test kadhi rimwe chete foil yakatsvuka nedudziro
.Samuple Duiluents: Izvo zvinongedzo ndeye 20mm ph7.4pbs
.Dispette
.Package insert
Zvigadzirwa zvinodiwa asi zvisina kupihwa
Sampu yekuunganidza mudziyo, Timer
Muenzaniso Unganidzo uye Kuchengetedza
Shandisa mudziyo wakachena wokutora kuti utore mitsva yemuenzaniso, uye akaedzwa pakarepo. Kana ikasakwanisa kuyedzwa pakarepo, ndapota yakachengetwa pa2-8 ° C ye 12hours kana Belows -15 ° C kwemwedzi mina.
Assay maitiro
1Tora kunze kweti sampling sampling, yakaiswa mumatanda emuenzaniso, wozoisa sampling tsvimbo kumashure, screw zvakaoma uye zunza zvakanaka, dzokorora chiito katatu. Kana kushandisa sampling inonamira pamusoro pe50mg inonyora sampuli, uye isa mune inonakisa sample tube ine sampuli yekufungidzira, uye screw zvakasimba.
2.Use disposable pipette sampling tora iyo inotetepa inotsemuka kubva kune anonzi madutu emurwere, wozowedzera 3 madonhwe (anenge 100ul) kune iyo fecal sampling tube uye zunza zvakanaka, isa parutivi.
3.Kutarisa kadhi rekuedza kubva kuhomwe yehombodo, chiise patafura yemubatanidzwa uye mucherechedze.
4.ReMuve Capple Kubva Mukuremera Tube uye Wedzera 3 Drips (About Lave (About 100ul) Pasina Muenzaniso Wemakadhi neKadhi rakapihwa, tanga nguva.
5.Mhedzisiro inofanirwa kuverengerwa mukati memaminitsi gumi nemashanu, uye hazvibvumirwe mushure memaminitsi gumi nemashanu.
Mhedzisiro yebvunzo uye kududzira
| Mhedzisiro yebvunzo | Kududzira | |
| ① | Red Refer red uye Red Control bandappear pane r dunhu uye c dunhu, hapana tsvukuBvunzo bhendi pane t dunhu. | Zvinoreva kuti zvirimo zvemukati zvevanhu faecessprotein iri pasi15 mp315μg / g, iyo irizvakajairika. |
| ② | Red Refer Red uye Red Control bandappear pane r dunhu uye c dunhu, uye iyoRuvara rweRed Refer red band yakasviba kupfuuraRed bvunzo bhendi. | Zviri mukati menzvimbo yevanhu calpolotectin isbetween 15μg / g uye 60μg / g. Izvo zvinogona kunge zvirimune yakajairika mwero, kana panogona kunge paine njodzi yeKutsamwisa matombo eBowl syndrome. |
| ③ | Red Refer Red uye Red Control bandappear pane r dunhu uye c dunhu, uye iyoRuvara rweRed Refer redhiti yakafanana neRed bvunzo bhendi. | Zvirimo zvehunhu hwevanhu calprotectin iri60μg / g, uye kune njodzi iripo yechirwere chehutu hosha. |
| ④ | Red Refer Red uye Red Control bandappear pane r dunhu uye c dunhu, uye iyoRuvara rweRed bvunzo bhendi rakasviba pane tsvukuRevhisi. | Zvinoratidza izvo zvemukati zvevanhu faecescalprotein zvinopfuura 60μg / g, uye ipapoInzvimbo inogara iripo yenguvo inopisachirwere. |
| ⑤ | Kana tsvuku rejista yebhendi uye tsvuku control bandis isingaonekwe kana kungoonekwa chete, iyo bvunzo ndeyeinoonekwa sevasina basa. | Dzokorora bvunzo uchishandisa kadhi idzva rekuyedza. |
Kuchengetera uye Kugadzikana
Iyo kit ndeye 24 mwedzi mahefu-hupenyu kubva pazuva rekugadzira. Chengetedza iyo isina kushandiswa kits pa 2-30 ° C. Usavhura pouchisimudzirwa yakavanzika kusvikira wagadzirira kuita bvunzo.
Yambiro uye Kuchera
1.The kit inofanira kuiswa chisimbiso uye akadzivirirwa kupokana nekunyorova1.
2.Do usashandise sampuli iyo inoiswa kureba kana kudzokorora kutonhora uye kuseka kuti uedze
3.Facal samples yakanyanya kana gobvu inogona kuita samples dzakaomeswa dzekutarisa kadhi, ndapota centrifuge sampuli yakadzama uye tora supernatant yekuedza.
4.misopariseni, yakawandisa kana shoma sampuli inogona kutungamira kumhedzisiro.
Kusimudzwa
1.Iye bvunzo mhedzisiro ndeyechiratidzo chekiriniki chete, haafanire kushandira chete kuongororwa kwekiriniki uye kurapwa, nhoroondo yekurapa, imwe yekuongorora, kupindurwa kwekurapa, nzira dzekurapa, efidmilogy uye zvimwe Ruzivo2.
2.this Reagent inongoshandiswa chete kune fecal bvunzo. Izvo zvinogona kunge zvisingawane mhedzisiro chaiyo kana ichishandiswa kune mamwe sampuli dzakadai sesimba uye urini uye nezvimwe.
Mareferensi
[1] National Clinical bvunzo maitiro (iyo yechitatu edition, 2006). Iyo Hutano hwehurumende.
. China chikafu uye madhiragi kutonga, kwete. 5 Kurongeka, 2014-07-30.
Kiyi yezviratidzo zvinoshandiswa:
 | Mu vitro diagnostic chero chishandiso |
 | Mugadziri |
 | Chitoro pa2-30 ℃ |
 | Zuva rekupera |
 | Usashandise |
 | Kungwarira |
 | Bvunza mirayiridzo yekushandisa |
Xiamen wiz biotech co., Ltd
Kero: 3-4 pasi, Kwete.16 Kuvaka, Bio-Medical Workist, 2030 Wengjioo West Road, Haicang District, 361026, Xiamen, China
Tel: + 86-592-6808278
Fax: + 86-592-6808279
