Diagnostic Kit(Colloidal Gold）nokuda kweCalprotectin
Yekushandisa in vitro diagnostic chete
Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo. Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.

ZVIRI KUSHANDISA
Diagnostic Kit yeCalprotectin(cal) ndeye colloidal goridhe immunochromatographic assay yekumisikidza semiquantitative yecal kubva mutsvina yevanhu, iyo ine yakakosha yekuongororwa kukosha kwechirwere chekuzvimba bowel. Muedzo uyu ndeye screening reagent. Yese yakanaka sampuli inofanirwa kusimbiswa nedzimwe nzira. Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete. Zvichakadaro, bvunzo iyi inoshandiswa IVD, zvimwe zviridzwa hazvidiwe.

SUMMARY
Cal is heterodimer, iyo inoumbwa neMRP 8 uye MRP 14. Inowanikwa mune neutrophils cytoplasm uye inoratidzwa pa mononuclear cell membranes. Cal is acute phase mapuroteni, ine yakanyatsogadzikana chikamu chingangoita vhiki imwe mutsvina yevanhu, yakatemwa kuve chiratidzo chekuzvimba bowel chirwere. Iyo kiti ndeye yakapfava, inoonekwa semiqualitative bvunzo inoona cal mutsvina yemunhu, ine yakakwirira yekuona senitivity uye yakasimba chaiyo. Muedzo wakavakirwa pane yakakwira yakapetwa kaviri masoja ekudzivirira chirwere sandwich reaction musimboti uye goridhe immunochromatographic assay yekuongorora matekiniki, inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

ZVINOTAURWA ZVINOITA
Iyo tambo ine anti cal coating McAb pane bvunzo dunhu uye mbudzi anti-tsuro IgG antibody panzvimbo yekudzora, iyo yakasungirirwa kune membrane chromatography pamberi. Lable pad yakaputirwa negoridhe rekolloidal rakanyorwa kuti anti cal McAb uye colloidal goridhe rakanyorwa rebbit IgG antibody pamberi. Paunenge uchiyedza sampuli yakanaka, iyo cal mumuenzaniso yakasanganiswa negoridhe colloidal yakanyorwa anti cal McAb, uye inogadzira immune yakaoma, sezvo ichitenderwa kutama ichitevedza bvunzo tambo, iyo cal conjugate complex inobatwa ne anti cal coating McAb pa membrane uye fomu. "anti cal coating McAb-cal-colloidal goridhe yakanyorwa kuti anti cal McAb" yakaoma, bhendi rekuedza rine ruvara rakaonekwa munzvimbo yekuyedza. Iko kusimba kwemavara kunoenderana zvakanaka necal content. Sample yakashata haiburitse bhendi rekuyedza nekuda kwekushaikwa kwecolloidal goridhe conjugate cal complex. Hazvina mhosva kuti cal iripo mumuenzaniso kana kwete, kune mutsara mutsvuku unoonekwa padunhu rereferenzi uye dunhu rekutonga, iro rinoonekwa semhando yemukati bhizinesi zviyero.

REAGENTS NEZVINHU ZVINOPIWA
25T package zvikamu:
.Kuedza kadhi rimwe nerimwe foil rakaiswa muhomwe ine desiccant
.Sample diluents: the ingredients is 20mM pH7.4PBS
.Dispette
.Package isa

ZVINHU ZVINODIWA ASI ZVISINA KUPIWA

Sample yekuunganidza mudziyo, timer

SAMPLE KUCHENGA UYE KUCHENGA
Shandisa mudziyo wakachena unoraswa kunhonga tsvina itsva, woongororwa nekukasika. Kana isingakwanisi kuongororwa nekukasira, ndapota ichengeterwe pa2-8 ° C kwe12hours kana kubhero -15 ° C kwemwedzi mina.

ASAY PROCEDURE
1.Bvisa sampling stick, wopinzwa mune tsvina sampuli, wozodzorera sampling stick, screw tight uye zunza zvakanaka, dzokorora chiito katatu. Kana kushandisa sampling inonamirwa ingangoita 50mg tsvina yesampuli, woisa mune tsvina yemuchubhu ine sampuro dilution, uye screw zvakasimba.
2.Shandisa inoraswa pipette sampling tora tsvina yakatetepa kubva kumurwere wemanyoka, wobva wawedzera madonhwe matatu (anenge 100uL) kune fecal sampling chubhu uye zunza zvakanaka, isa parutivi.
3.Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.
4.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample verticaly uye zvishoma nezvishoma mumuenzaniso wakanaka wekadhi nekupa dispette, tanga nguva.
5.Chigumisiro chinofanira kuverengwa mukati me10-15 maminitsi, uye hazvina kukodzera mushure memaminitsi gumi nemashanu.

TEST RESULTS UYE DUDZIRIRO

KUCHENGA UYE KUgadzikana
Iyo kit ndeye 24 mwedzi pasherufu-hupenyu kubva pazuva rekugadzirwa. Chengetedza makiti asina kushandiswa pa2-30°C. Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo.
YAMBIRO UYE CHENJERERO
1.Ikiti inofanira kuiswa chisimbiso uye kudzivirirwa pakunyorova¹.
2.Usashandisa sampuli inoiswa yakarebesa kana kudzokororwa nechando uye kunyunguduka kuti uedze
3.Fecal samples yakawandisa kana ukobvu inogona kuita diluted samples tsvina yebvunzo kadhi, ndapota centrifuge sampu yakanyungudutswa uye tora iyo supernatant yekuyedza.
4.Misoperation, yakawandisa kana sampuli shoma inogona kutungamirira kumigumisiro yekutsauka.

LIMITATION
1.Iyi mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete, haifanire kushanda seyo chete hwaro hwekuongororwa kwekiriniki uye kurapwa, varwere vekiriniki manejimendi inofanirwa kutariswa zvakakwana pamwe chete nezviratidzo zvayo, nhoroondo yekurapa, kumwe kuongororwa kwerabhoritari, mhinduro yekurapa, epidemiology nezvimwe. ruzivo².
2.Iyi reagent inoshandiswa chete pakuongorora fecal. Iyo inogona kusawana mhedzisiro chaiyo kana ichishandiswa kune mamwe masampuli senge mate uye weti uye nezvimwe.