Diagnostic Kit yeProcalcitonin

(fluorescence immunochromatographic assay)

Yekushandisa in vitro diagnostic chete

Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo. Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.

ZVIRI KUSHANDISA

Diagnostic Kit yeProcalcitonin (fluorescence immunochromatographic assay) ndeye fluorescence immunochromatographic assay yehuwandu hwekuonekwa kweProcalcitonin (PCT) muserum yevanhu kana plasma, inoshandiswa pakubatsira kuongororwa kwehutachiona hwehutachiona uye sepsis. Yese yakanaka sampuli inofanirwa kusimbiswa nedzimwe nzira. Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete.

SUMMARY

Procalcitonin inoumbwa ne116 amino acids uye huremu hwayo hwemamorekuru i12.7KD. PCT inoratidzwa nemasero e-neuroendocrine uye yakaputsika nema enzymes mu (immature) calcitonin, carboxy-terminating peptide, uye amino kupedza peptide. Vanhu vane hutano vane zvishoma zvePCT muropa ravo, izvo zvinogona kuwedzerwa zvakanyanya mushure mekutapukirwa kwebhakitiriya. Kana sepsis ikaitika mumuviri, matishu mazhinji anogona kuratidza PCT, saka PCT inogona kushandiswa sechiratidzo chekufungidzira che sepsis. Kune vamwe varwere vane hutachiona hwehutachiona, PCT inogona kushandiswa sechiratidzo chekusarudzwa kwemishonga inorwisa mabhakitiriya uye kutonga kwakanaka.

ZVINOTAURWA ZVINOITA

Iyo membrane yechishandiso chekuyedza yakavharwa neanti PCT antibody padunhu rekuyedza uye mbudzi anti tsuro IgG antibody panzvimbo yekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti PCT antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka, iyo PCT antigen mumuenzaniso inosanganisa nefluorescence yakanyorwa kuti anti PCT antibody, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, iyo yakaoma kuyerera ikananga kune inonwisa bepa, apo yakaoma yakapfuura dunhu rekuyedzwa, yakasanganiswa neanti PCT coating antibody, inoumba nyowani yakaoma. PCT nhanho yakanyatsoenderana neiyo fluorescence chiratidzo, uye kusungwa kwePCT mumuenzaniso kunogona kuwonekwa nefluorescence immunoassay assay.